RDRP_I97A1
ID RDRP_I97A1 Reviewed; 758 AA.
AC Q9WLS3; Q9ICX8; Q9WA97;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 23-FEB-2022, entry version 85.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000255|HAMAP-Rule:MF_04065};
DE EC=2.7.7.48 {ECO:0000255|HAMAP-Rule:MF_04065};
DE AltName: Full=Polymerase basic protein 1 {ECO:0000255|HAMAP-Rule:MF_04065};
DE Short=PB1 {ECO:0000255|HAMAP-Rule:MF_04065};
DE AltName: Full=RNA-directed RNA polymerase subunit P1 {ECO:0000255|HAMAP-Rule:MF_04065};
GN Name=PB1 {ECO:0000255|HAMAP-Rule:MF_04065};
OS Influenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX NCBI_TaxID=130763;
OH NCBI_TaxID=8782; Aves.
OH NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH NCBI_TaxID=9694; Panthera tigris (Tiger).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=9430591; DOI=10.1126/science.279.5349.393;
RA Subbarao K., Klimov A., Katz J., Regnery H., Lim W., Hall H., Perdue M.,
RA Swayne D., Bender C., Huang J., Hemphill M., Rowe T., Shaw M., Xu X.,
RA Fukuda K., Cox N.;
RT "Characterization of an avian influenza A (H5N1) virus isolated from a
RT child with a fatal respiratory illness.";
RL Science 279:393-396(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=9658115; DOI=10.1128/jvi.72.8.6678-6688.1998;
RA Suarez D.L., Perdue M.L., Cox N., Rowe T., Bender C., Huang J.,
RA Swayne D.E.;
RT "Comparisons of highly virulent H5N1 influenza A viruses isolated from
RT humans and chickens from Hong Kong.";
RL J. Virol. 72:6678-6688(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=10920197; DOI=10.1073/pnas.160270697;
RA Lin Y.P., Shaw M., Gregory V., Cameron K., Lim W., Klimov A., Subbarao K.,
RA Guan Y., Krauss S., Shortridge K., Webster R., Cox N., Hay A.;
RT "Avian-to-human transmission of H9N2 subtype influenza A viruses:
RT relationship between H9N2 and H5N1 human isolates.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:9654-9658(2000).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000255|HAMAP-Rule:MF_04065}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|HAMAP-Rule:MF_04065};
CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus).
CC Interacts (via C-terminus) with PB2 (via N-terminus); this interaction
CC is essential for transcription initiation. {ECO:0000255|HAMAP-
CC Rule:MF_04065}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04065}.
CC Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04065}.
CC -!- PTM: Phosphorylated by host PRKCA. {ECO:0000255|HAMAP-Rule:MF_04065}.
CC -!- SIMILARITY: Belongs to the influenza viruses polymerase PB1 family.
CC {ECO:0000255|HAMAP-Rule:MF_04065}.
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DR EMBL; AF036362; AAC34271.1; -; Genomic_RNA.
DR EMBL; AF046094; AAC32096.1; -; Genomic_RNA.
DR EMBL; AJ404633; CAB95863.1; -; Genomic_RNA.
DR PDB; 3CM8; X-ray; 2.90 A; B=1-25.
DR PDBsum; 3CM8; -.
DR SMR; Q9WLS3; -.
DR EvolutionaryTrace; Q9WLS3; -.
DR Proteomes; UP000008587; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:UniProtKB-UniRule.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Eukaryotic host gene expression shutoff by virus;
KW Eukaryotic host transcription shutoff by virus; Host cytoplasm;
KW Host gene expression shutoff by virus; Host nucleus;
KW Host-virus interaction; Inhibition of host RNA polymerase II by virus;
KW Nucleotide-binding; Nucleotidyltransferase; Phosphoprotein;
KW RNA-directed RNA polymerase; Transferase; Viral RNA replication;
KW Viral transcription.
FT CHAIN 1..758
FT /note="RNA-directed RNA polymerase catalytic subunit"
FT /id="PRO_0000078752"
FT DOMAIN 286..483
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04065"
FT REGION 53..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..256
FT /note="Promoter-binding site"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04065"
FT MOTIF 187..195
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04065"
FT MOTIF 203..216
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04065"
FT CONFLICT 119
FT /note="M -> V (in Ref. 2; AAC32096 and 3; CAB95863)"
FT CONFLICT 189
FT /note="R -> G (in Ref. 2; AAC32096)"
FT CONFLICT 610
FT /note="G -> C (in Ref. 2; AAC32096 and 3; CAB95863)"
FT CONFLICT 660
FT /note="V -> A (in Ref. 3; CAB95863)"
FT CONFLICT 712
FT /note="S -> P (in Ref. 2; AAC32096)"
FT CONFLICT 757
FT /note="G -> R (in Ref. 3; CAB95863)"
FT HELIX 5..10
FT /evidence="ECO:0007829|PDB:3CM8"
SQ SEQUENCE 758 AA; 86283 MW; 97223D885FF353E2 CRC64;
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS EKGRWTTNTE
TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GLFENSCLET MEVVQQTRMD
KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLTANES GRLIDFLKDV MESMDKEEME
ITTHFQRKRR VRDNMTKKMV TQRTIGKKKQ RLTKKSYLIR ALTLNTMTKD AERGKLKRRA
IATPGMQIRG FVHFVEALAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF
TVTGDNTKWN ENQNPRIFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR LGKGYMFESK
SMKLRTQIPA EMLANIDLKY FNESTRKKIE KIRPLLVEGT ASLSPGMMMG MFNMLSTVLG
VSILNLGQKR YTKTTYWWDG LQSSDDFALI VNAPNHEGIQ AGVDRFYRTC KLVGINMSKK
KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG
PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFELKKLW EQTRSKAGLL VSDGGPNLYN
IRNLHIPEVG LKWELMDEDY QGRLCNPLNP FVSHKEVESV NNAVVMPAHG PAKSMEYDAV
ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCTLFEKFF PSSSYRRPVG ISSMMEAMVS
RARIDARIDF ESGRIKKEEF AEILKICSTI EELGRQGK
