RDRP_IPNVJ
ID RDRP_IPNVJ Reviewed; 845 AA.
AC P22173;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=RNA-directed RNA polymerase;
DE Short=RDRP;
DE EC=2.7.7.48;
DE AltName: Full=Protein VP1;
GN Name=VP1;
OS Infectious pancreatic necrosis virus (strain Jasper) (IPNV).
OC Viruses; Riboviria; Orthornavirae; Birnaviridae; Aquabirnavirus.
OX NCBI_TaxID=11003;
OH NCBI_TaxID=8022; Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OH NCBI_TaxID=8028; Salmo.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=1901682; DOI=10.1016/0042-6822(91)90887-h;
RA Duncan R., Mason C.L., Nagy E., Leong J.-A., Dobos P.;
RT "Sequence analysis of infectious pancreatic necrosis virus genome segment B
RT and its encoded VP1 protein: a putative RNA-dependent RNA polymerase
RT lacking the Gly-Asp-Asp motif.";
RL Virology 181:541-552(1991).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is found both free and
CC covalently attached to the genomic RNA. May also contain guanylyl and
CC methyl transferase activities (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- SUBUNIT: Interacts with VP3 in the cytoplasm. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Minor amounts are
CC incorporated in the virion. {ECO:0000250}.
CC -!- PTM: Exists in multiple phosphorylated forms.
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DR EMBL; M58756; AAA46243.1; -; Genomic_RNA.
DR PIR; A37946; RRXSJA.
DR RefSeq; NP_047197.1; NC_001916.1.
DR PDB; 2YI8; X-ray; 2.30 A; A/B/C/D/E=1-790.
DR PDB; 2YI9; X-ray; 2.20 A; A/B/C/D/E=1-790.
DR PDB; 2YIA; X-ray; 3.02 A; A/B/C/D/E/F/G/H=1-790.
DR PDB; 2YIB; X-ray; 3.80 A; A/B/C=1-845, D=1-687.
DR PDB; 3ZED; X-ray; 2.20 A; A/B/C=1-845.
DR PDBsum; 2YI8; -.
DR PDBsum; 2YI9; -.
DR PDBsum; 2YIA; -.
DR PDBsum; 2YIB; -.
DR PDBsum; 3ZED; -.
DR SMR; P22173; -.
DR PRIDE; P22173; -.
DR GeneID; 956514; -.
DR KEGG; vg:956514; -.
DR BRENDA; 2.7.7.48; 6986.
DR EvolutionaryTrace; P22173; -.
DR Proteomes; UP000007248; Genome.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0018144; P:RNA-protein covalent cross-linking; IEA:UniProtKB-KW.
DR GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR007100; RNA-dir_pol_birnavirus.
DR InterPro; IPR007098; RNA-dir_pol_mononegavirus.
DR Pfam; PF04197; Birna_RdRp; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS50524; RDRP_DSRNA_BIR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Covalent protein-RNA linkage; GTP-binding;
KW Nucleotide-binding; Nucleotidyltransferase; Phosphoprotein;
KW Reference proteome; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication; Virion.
FT CHAIN 1..845
FT /note="RNA-directed RNA polymerase"
FT /id="PRO_0000221963"
FT DOMAIN 384..588
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT REGION 686..706
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 799..845
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 248..255
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT HELIX 14..18
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 23..25
FT /evidence="ECO:0007829|PDB:2YIA"
FT TURN 28..30
FT /evidence="ECO:0007829|PDB:3ZED"
FT HELIX 45..54
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 65..72
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 73..76
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 78..80
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 81..85
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 91..102
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:3ZED"
FT HELIX 125..137
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 143..156
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 157..160
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 164..179
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 180..182
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 187..190
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 194..204
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 221..224
FT /evidence="ECO:0007829|PDB:2YI9"
FT TURN 231..233
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 256..278
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 283..293
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 295..298
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 299..304
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 307..310
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 311..313
FT /evidence="ECO:0007829|PDB:2YI9"
FT TURN 314..316
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 320..323
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 326..340
FT /evidence="ECO:0007829|PDB:2YI9"
FT TURN 347..349
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 350..354
FT /evidence="ECO:0007829|PDB:2YI9"
FT TURN 361..363
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 364..377
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 382..386
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 389..394
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 397..403
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 406..409
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 412..425
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 434..436
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 438..446
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 448..452
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 456..460
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 462..465
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 476..493
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 502..505
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 507..511
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 515..521
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 524..533
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 537..539
FT /evidence="ECO:0007829|PDB:2YI9"
FT TURN 540..543
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 556..560
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 563..568
FT /evidence="ECO:0007829|PDB:2YI9"
FT TURN 569..572
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 573..578
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 580..588
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 596..599
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 604..618
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 621..623
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 625..645
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 650..654
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 656..659
FT /evidence="ECO:0007829|PDB:2YI9"
FT TURN 661..665
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 677..684
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 692..694
FT /evidence="ECO:0007829|PDB:2YI9"
FT STRAND 699..701
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 706..714
FT /evidence="ECO:0007829|PDB:2YI9"
FT TURN 715..718
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 724..732
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 737..739
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 740..751
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 757..771
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 775..777
FT /evidence="ECO:0007829|PDB:2YI9"
FT HELIX 781..790
FT /evidence="ECO:0007829|PDB:2YI9"
SQ SEQUENCE 845 AA; 94467 MW; 45C392DF3AAF767A CRC64;
MSDIFNSPQN KASILTALMK STTGDVEDVL IPKRFRPAKD PLDSPQAAAQ FLKDNKYRIL
RPRAIPTMVE LETDAALPRL RQMVEDGKLK DTVSVPEGTT AFYPKYYPFH KPDHDEVGTF
GAPDITLLKQ LTFFLLENDF PTGPETLRQV REAIATLQYG SGSYSGQLNR LLAMKGVATG
RNPNKTPKTV GYTNEQLAKL LEQTLPINTP KHEDPDLRWA PSWLINYTGD LSTDKSYLPH
VTIKSSAGLP YIGKTKGDTT AEALVLADSF IRDLGRAATS ADPEAGVKKT ITDFWYLSCG
LLFPKGERYT QVDWDKKTRN IWSAPYPTHL LLSMVSTPVM NESKLNITNT QTPSLYGFSP
FHGGMDRIMT IIRDSLDNDE DLVMIYADNI YILQDNTWYS IDLEKGEANC TPQHMQAMMY
YLLTRGWTNE DGSPRYNPTW ATFAMNVAPS MVVDSSCLLM NLQLKTYGQG SGNAFTFLNN
HLMSTIVVAE WVKAGKPNPM TKEFMDLEEK TGINFKIERE LKNLRETIVE AVETAPQDGY
LADGSDLPPI RPGKAVELDL LGWSAIYSRQ MEMFVPVLEN ERLIASAAYP KGLENKALAR
KPGAEIAYQI VRYEAIRLVG GWNNPLLETA AKHMSLDKRK RLEVKGIDVT GFLDDWNNMS
EFGGDLEGIT LSEPLTNQTL VDINTPLDSF DPKARPQTPR SPKKTLDEVT TAITSGTYKD
PKSAVWRLLD QRTKLRVSTL RDQALALKPA SSSVDNWAEA TEELAQQQQL LMKANNLLKS
SLTETREALE TIQSDKIIAG KSNPEKNPGT AANPVVGYGE FSEKIPLTPT QKKNAKRREK
QRRNQ
