ID   RDRP_IPNVS              Reviewed;         844 AA.
AC   P22174;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            Short=RDRP;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein VP1;
GN   Name=VP1;
OS   Infectious pancreatic necrosis virus (strain Sp) (IPNV).
OC   Viruses; Riboviria; Orthornavirae; Birnaviridae; Aquabirnavirus.
OX   NCBI_TaxID=11005;
OH   NCBI_TaxID=8022; Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OH   NCBI_TaxID=8028; Salmo.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1901682; DOI=10.1016/0042-6822(91)90887-h;
RA   Duncan R., Mason C.L., Nagy E., Leong J.-A., Dobos P.;
RT   "Sequence analysis of infectious pancreatic necrosis virus genome segment B
RT   and its encoded VP1 protein: a putative RNA-dependent RNA polymerase
RT   lacking the Gly-Asp-Asp motif.";
RL   Virology 181:541-552(1991).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which is found both free and
CC       covalently attached to the genomic RNA. May also contain guanylyl and
CC       methyl transferase activities (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with VP3 in the cytoplasm. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Minor amounts are
CC       incorporated in the virion. {ECO:0000250}.
CC   -!- PTM: Exists in multiple phosphorylated forms.
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DR   EMBL; M58757; AAA46244.1; -; Genomic_RNA.
DR   PIR; B37946; RRXSSP.
DR   SMR; P22174; -.
DR   Proteomes; UP000007213; Genome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0018144; P:RNA-protein covalent cross-linking; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007100; RNA-dir_pol_birnavirus.
DR   InterPro; IPR007098; RNA-dir_pol_mononegavirus.
DR   Pfam; PF04197; Birna_RdRp; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50524; RDRP_DSRNA_BIR; 1.
PE   3: Inferred from homology;
KW   Covalent protein-RNA linkage; GTP-binding; Nucleotide-binding;
KW   Nucleotidyltransferase; Phosphoprotein; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication; Virion.
FT   CHAIN           1..844
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000221964"
FT   DOMAIN          384..588
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          820..844
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         248..255
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   844 AA;  94089 MW;  0E8D7B9553A7A456 CRC64;
     MSDIFNSPQN KASILNALMK STQGDVEDVL IPKRFRPAKD PLDSPQAAAA FLKEHKYRIL
     RPRAIPTMVE IETDAALPRL AAMVDDGKLK EMVNVPEGTT AFYPKYYPFH RPDHDDVGTF
     GAPDITLLKQ LTFFLLENDF PTGPETLRQV REAIATLQYG SGSYSGQLNR LLAMKGVATG
     RNPNKTPLAV GYTNEQMARL MEQTLPINPP KNEDPDLRWA PSWLIQYTGD ASTDKSYLPH
     VTAKSSAGLP YIGKTKGDTT AEALVLADSF IRDLGKAATS ADPGAAAKKV LSDFWYLSCG
     LLFPKGERYT QKDWDLKTRN IWSAPYPTHL LLSMVSSPVM DESKLNITNT QTPSLYGFSP
     FHGGINRIMT IIREHLDQEQ DLVMIYADNI YILQDNTWYS IDLEKGEANC TPQHMQAMMY
     YRLTREWTNE DGSPRYNPTW ATFAMYVGPS MVVDSTCLLM NLQLKTTGQG SGNAFTFLNN
     HLMSTIVVAE WHKAGRPNPM SKEFMDLEAK TGINFKIERE LKDLRSIIME AVDTAPLDGY
     LADGSDLPPR VPGKAVELDL LGWSAVYSRQ LEMFVPVLEN ERLIASVAYP KGLENKSLAR
     KPGAEIAYQI VRYEAIRLIG GWNNPLIETA AKHMSLDKRK RLEVKGIDVT GFLDDWNTMS
     EFGGDLEGIS LTAPLTNQTL LDINTPETEF DVKDRPPTPR SPGKTLAEVT AAITSGTYKD
     PKSAVWRLLD QRTKLRVSTL RDHAHALKPA ASTSDFWGDA TEELAEQQQL LMKANNLLKS
     SLTEAREALE TVQSDKIISG KTSPEKNPGT AANPVVAYGE FSEKIPLTPT QKKNAKRREK
     QRRN