ID   RDRP_ISAV8              Reviewed;         708 AA.
AC   Q8V3T6;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   23-FEB-2022, entry version 74.
DE   RecName: Full=RNA-directed RNA polymerase catalytic subunit;
DE            EC=2.7.7.48;
DE   AltName: Full=Polymerase basic protein 1;
DE            Short=PB1;
DE   AltName: Full=Protein 1;
DE            Short=P1;
DE   AltName: Full=RNA-directed RNA polymerase subunit P1;
GN   Name=Segment-2 {ECO:0000303|PubMed:20979983};
OS   Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99)
OS   (ISAV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Isavirus.
OX   NCBI_TaxID=652965;
OH   NCBI_TaxID=8049; Gadus morhua (Atlantic cod).
OH   NCBI_TaxID=8019; Oncorhynchus kisutch (Coho salmon) (Salmo kisutch).
OH   NCBI_TaxID=8022; Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OH   NCBI_TaxID=8060; Pollachius virens (Saithe) (Gadus virens).
OH   NCBI_TaxID=8030; Salmo salar (Atlantic salmon).
OH   NCBI_TaxID=8032; Salmo trutta (Brown trout).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=11807235; DOI=10.1099/0022-1317-83-2-421;
RA   Clouthier S.C., Rector T., Brown N.E., Anderson E.D.;
RT   "Genomic organization of infectious salmon anaemia virus.";
RL   J. Gen. Virol. 83:421-428(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=RPC/NB 02-0775-14;
RA   Kibenge F.S., Kibenge M.J., Qian B.;
RT   "Infectious salmon anaemia virus genomic RNA segment 2.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   REVIEW.
RX   PubMed=20979983; DOI=10.1016/j.virusres.2010.10.021;
RA   Cottet L., Rivas-Aravena A., Cortez-San Martin M., Sandino A.M.,
RA   Spencer E.;
RT   "Infectious salmon anemia virus--genetics and pathogenesis.";
RL   Virus Res. 155:10-19(2011).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC       replication and transcription of virus segments. Binds the promoter
CC       sequence of the encapsidated viral RNA. Displays an endonuclease
CC       activity involved in cap-stealing. Cleaves cellular pre-mRNA to
CC       generate primers for viral transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: The RNA polymerase is composed of three subunits: PB1, PB2 and
CC       PA. {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF404346; AAL67962.1; -; Viral_cRNA.
DR   EMBL; DQ676937; ABG81414.1; -; Viral_cRNA.
DR   RefSeq; YP_145804.1; NC_006503.1.
DR   SMR; Q8V3T6; -.
DR   PRIDE; Q8V3T6; -.
DR   GeneID; 3170817; -.
DR   KEGG; vg:3170817; -.
DR   Proteomes; UP000008772; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR   InterPro; IPR001407; RNA_pol_PB1_influenza.
DR   Pfam; PF00602; Flu_PB1; 1.
DR   PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR   PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..708
FT                   /note="RNA-directed RNA polymerase catalytic subunit"
FT                   /id="PRO_0000403918"
FT   DOMAIN          281..467
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   708 AA;  80526 MW;  3D4128B467CC899B CRC64;
     METLVGGLLT GEDSLISMSN DVSCLYVYDG PMRVFSQNAL MPTLQSVKRS DQFSKGKTKR
     FIIDLFGMKR MWDIGNKQLE DENLDETVGV ADLGLVKYLI NNKYDEAEKT SLRKSMEEAF
     EKSMNEEFVV LNKGKSANDI ISDTNAMCKF CVKNWIVATG FRGRTMSDLI EHHFRCMQGK
     QEVKGYIWKH KYNERLKRKQ LSKEEVKFDR EEYTSRSFRL LSFLKNSERT KLEPRAVFTA
     GVPWRAFIFV LEQTMLVVNK LDPNSVIWMG SDAKINTTNS RIKEIGMKNQ GQTLVTLTGD
     NSKYNESMCP EVMMVFLREL GIKGPMLEVL DYALWQFSQK SVKPVAPIKK RTGKSTVVIK
     ADSVKECRDA FNEKELELIQ GVEWMDDGFV RVRRGMLMGM ANNAFTTAST IASSFSFTPE
     AVYTLQSSDD FVTGSCGRDV QHARQRLEMA LKVSKAAGLN VSQKKSFYVE GTTFEFNSMF
     VRDGKVMANG GNFENMTVPG GLGPSTDLFV VGKQARNSML RGNLSFSQAM EMCKIGITNV
     EKVYYGNRKY QELKNEIREK CGEETMSIPE SMGGDRKPRP WELPQSFDGI ALKEAVNRGH
     WKAAKYIKSC CSIEFDEEGD QSWDTSKTAL VVIRKNETDM RRRTVKTRNP KDKIFNDAMN
     KAKRMYETVV DRNPLLGLKG KGGRLTVKDL KARKLIDEVE VVKKKKHV