ID   RDRP_LDCPR              Reviewed;        1225 AA.
AC   Q91IE0;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   29-SEP-2021, entry version 51.
DE   RecName: Full=RNA-directed RNA polymerase VP2;
DE            EC=2.7.7.48;
GN   Name=S2;
OS   Lymantria dispar cypovirus 1 (isolate Rao) (LdCPV-1).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Spinareovirinae; Cypovirus.
OX   NCBI_TaxID=648169;
OH   NCBI_TaxID=13123; Lymantria dispar (Gypsy moth) (Porthetria dispar).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Rao S., Shapiro M., Lynn D., Hagiwara K., Blackmon B., Fang G.,
RA   Carner G.R.;
RT   "Identification of dsRNA electrophoretypes of two cypoviruses from a dual
RT   infection in gypsy moth, Lymantria dispar.";
RL   Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-directed RNA polymerase that is involved in transcription
CC       and genome replication. Following infection, it catalyzes the synthesis
CC       of fully conservative plus strands. After core assembly, which consists
CC       in recruitment of one capped plus-strand for each genomic segments and
CC       polymerase complexes, the polymerase switches mode and catalyzes the
CC       synthesis of complementary minus-strands (By similarity).
CC       {ECO:0000255|PROSITE-ProRule:PRU00539}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with VP6. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the reoviridae RNA-directed RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; AF389463; AAK73521.1; -; Genomic_RNA.
DR   RefSeq; NP_149147.1; NC_003017.1.
DR   SMR; Q91IE0; -.
DR   GeneID; 2598189; -.
DR   KEGG; vg:2598189; -.
DR   Proteomes; UP000006712; Genome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR007097; RNA-dir_pol_reovirus.
DR   PROSITE; PS50523; RDRP_DSRNA_REO; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1225
FT                   /note="RNA-directed RNA polymerase VP2"
FT                   /id="PRO_0000403202"
FT   DOMAIN          497..727
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   1225 AA;  138847 MW;  614964068B1AB37D CRC64;
     MLPNTELHTT EFSKTRKFTR QSFKQIEQLT ASLANDRVAR HKFLFNNSLP LISDYSGEDS
     NGHQLQATIK IPDEITNPKE YDPSDYPLAE DESFFKQGHK YDYLVTFRAG SLTNTYEPKT
     KMYKLHAALD RLMHVKQRKS RFADLWRELC AVIASLDVWY QTTNYPLRTY VKFLFHKGDE
     FPFYESPSQD KIVFNDKSVA SILPTFVYTC CQVGTAIMSG ILTHVESIAA MNHFLHCAKD
     SYIDEKLKVK GIGRSWYQEA LHNVGQVTVP VWSQFNEIIG HRTKNTSEPH FVSATFTALR
     AKRAELLYSE FNDYINRALR LSKTQNDVAN YYAACRAMTN DGTFLATLTE LSLDAAVFPR
     IEQRLVTRPA VLMSNARHES LRQKYTDGVG SIAQSYLSSF TDEVARRVNG IHHDEAWLNF
     LTTSSPGRKL TEIEKLEVGG DVAAWSNSRI VMQAVFAREY RTPERIFKSL KAPIKLVERQ
     QSDRRQRAIS GLDNDRLFLS FMPYTIGKQI YDLNDNAAQG KQAGNAFDIG EMLYWTSQRN
     VLLSSIDVAG MDASVTTNTK DIYNTFVLDV ASKCTVPRFG PYYAKNMEVF EVGKRQSQVK
     YVNAAWQACA LEAANSQTST SYESEIFGQV KNAEGTYPSG RADTSTHHTV LLQGLVRGNE
     LKRASDGKNS CLATIKILGD DIMEIFQGSQ DDTHHHAVSN ANVLNESGFA TTAELSQNSI
     VLLQQLVVNG TFWGFADRIS LWTREDTKDI GRLNLAMMEL NALLDDLLFR VRRPEGLKML
     GFFCGAICLR RFTLSVDNNL YDSTYNSLSK YMTLIKYDKN PDFDSTLMSL ILPLTWLFMP
     RGGEYPAYPF ERRDGTFTED ESMFTARGAY KRRLLYDISN IREMIQQNSL ALDDELLHEY
     GFTGASLLID LNILDLIDEV KKEDISPVKV SELATSLEQL GKLGEREKSR RAASDLKVRG
     HALSNDIVYG YGLQEKIQKS AMATKETTVQ SKRISLRLHE VIATKTRDYR IPTTPADALH
     LYEFEGEEVV MDLLPHAKHT SYSNLAYNMS FGSDGWFAFA LLGGLDRSAI LLRLDVASIR
     GNYHKFSYDD PVFKQGYKIY KSDATSLDDF FIAISAGPKE QGILLRAFAY YSLYGNVEYH
     YVLSPRQLFF LSDNPVSAER LVRIPPSYYV STQCRALYNI FSYLHILRSI TSHEGKRLKM
     VLHAGLIAYV RGTSSSAILP EADTV