ID   RDRP_MBVLF              Reviewed;         420 AA.
AC   Q9YPD5;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   29-SEP-2021, entry version 62.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
GN   ORFNames=ORF3;
OS   Mushroom bacilliform virus (isolate Australia/AUS LF-1) (MBV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Sobelivirales; Barnaviridae; Barnavirus.
OX   NCBI_TaxID=650482;
OH   NCBI_TaxID=5341; Agaricus bisporus (White button mushroom).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8030251; DOI=10.1006/viro.1994.1412;
RA   Revill P.A., Davidson A.D., Wright P.J.;
RT   "The nucleotide sequence and genome organization of mushroom bacilliform
RT   virus: a single-stranded RNA virus of Agaricus bisporus (Lange) Imbach.";
RL   Virology 202:904-911(1994).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- PTM: Might be cleaved to release the mature protein(s). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U07551; AAA53090.1; -; Genomic_RNA.
DR   RefSeq; NP_042510.2; NC_001633.1.
DR   GeneID; 1497107; -.
DR   KEGG; vg:1497107; -.
DR   Proteomes; UP000006824; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR001795; RNA-dir_pol_luteovirus.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   Pfam; PF02123; RdRP_4; 2.
DR   PRINTS; PR00914; LVIRUSRNAPOL.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..420
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000402444"
FT   DOMAIN          199..325
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   420 AA;  46914 MW;  1E5A910DCDE5A72F CRC64;
     MDSGLYPKTG APTWFYGWSK QREGGFSDFG SEIFRLRALP PLKAEIFRGI REVVEYNITG
     DSHPGYPWCK LGSDNKAVLT GFGDLIWDEV AKRFNNMLGY GDAIFSMTPS ELVQNGICDA
     VKVFIKQEPH SLEKVNAGRL RIIAAVGLVD QIVTRLLCMK QNNAEIDCWE SCPSAPGMGL
     NDEGLRTLYS TAQVMAEHGT ICETDISGWD WSVQQWELDS DARLRTQLAG EEIGGYLNFF
     LRVHAYVVGH SVFVMPDGEM LEQTVPGGQL SGDYNTSSSN SRMRVIATMF ARYLAGQVSG
     FPLLGIKAMG DDSFEIWFKG LEEYLGKMGH TVKMCVQRPG LVGFEFCSQV FLGLGIAYPV
     DFSKTLYRFL SHHPADPKYS EYRAQLMYYF RHLPSSTLQK VIRLAGARVE RAQKLATSSN