ID   RDRP_MPRVN              Reviewed;        1371 AA.
AC   Q1I0V0;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   23-FEB-2022, entry version 35.
DE   RecName: Full=RNA-directed RNA polymerase VP2;
DE            EC=2.7.7.48;
GN   Name=S2;
OS   Micromonas pusilla reovirus (isolate Netherlands/2005) (MpRV).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Mimoreovirus.
OX   NCBI_TaxID=649596;
OH   NCBI_TaxID=38833; Micromonas pusilla (Picoplanktonic green alga) (Chromulina pusilla).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=16603541; DOI=10.1099/vir.0.81584-0;
RA   Attoui H., Jaafar F.M., Belhouchet M., de Micco P., de Lamballerie X.,
RA   Brussaard C.P.;
RT   "Micromonas pusilla reovirus: a new member of the family Reoviridae
RT   assigned to a novel proposed genus (Mimoreovirus).";
RL   J. Gen. Virol. 87:1375-1383(2006).
CC   -!- FUNCTION: RNA-directed RNA polymerase that is involved in transcription
CC       and genome replication. Following infection, it catalyzes the synthesis
CC       of fully conservative plus strands. After core assembly, which consists
CC       in recruitment of one capped plus-strand for each genomic segments and
CC       polymerase complexes, the polymerase switches mode and catalyzes the
CC       synthesis of complementary minus-strands (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the reoviridae RNA-directed RNA polymerase
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ126102; AAZ94042.1; -; Genomic_RNA.
DR   RefSeq; YP_654545.1; NC_008172.1.
DR   PRIDE; Q1I0V0; -.
DR   GeneID; 5076664; -.
DR   KEGG; vg:5076664; -.
DR   Proteomes; UP000000349; Genome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Hydrolase; Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication; Virion.
FT   CHAIN           1..1371
FT                   /note="RNA-directed RNA polymerase VP2"
FT                   /id="PRO_0000404160"
SQ   SEQUENCE   1371 AA;  154690 MW;  DD4F6F29DA411B0A CRC64;
     MSRKLGSNAP TPMHLRLGNR YETIEQLFNK TRDINRPNKQ QDVEIANTQE TQWMHACKLA
     LDLATEEQFE RAVSIVKEWS IEDRKHDNPT ESSYFPVIIR SSKEAEFPFK ERSAVLTQYG
     MDALLEARLN DPSGKRGVDA EESKLGFINI ETMNVKINGD YVTVNGVDLP KWSADNDASA
     KVGVTCSEAW FGARDARWTE ACRHLPFSLA STYVTWLMLL TEQAAGNDKF NIDGHGFTNL
     LTGLIYLASE WATRTSKSRL KKNVDIKGRV LLESWIKGNP PAIPSWMDDW GKPMKYKYVT
     ESDVGIKPHN DKGLAEWSKE AQLRNTPEGA DTESLLRSRI PFTEADSGPP YITDSALTTS
     GPLRAFLCYS TAVECCGSIH LRGFTEYYKC VLHYLGTQPG KIGKQISKVS SPVARALGAT
     SKEVMVPRRV QPSEHVWCDE TDCVTVKFED NYVLQLMCAS YDIPTNWESL ENKMFENELM
     GHCLRHIYDE YKHEHVNIHN SDLFDVITHL TNAGSAEGYV RSCLATLPVD GYMSHMKRIA
     LEGIDSSEDK EVSQYERVHW DAIMRTILPP TPEGAVQELK ALSNSRSGGA DRIRFKSDRN
     TALANSDRET DAPFVMSSNR KNSTIAMAAH LILNPVTLKH RSSYEVPLPT GIRSVPARIL
     RLIYNMPLPM QSLLQPLYKA MKAYMKRPTH NERYQLALNK GVVVHDSRTL INNSINCVHN
     PRLVSIADDA SKLDQHEGPR ARNVLIDALE AIPIDNGFAD EFGDSYTDLM RNMLAGWNTN
     YFAFNVDAEQ KQVMSVDTDP SGALITAEKN TVTSAGIQDM IIARTNIPQR DYLWGDDAYA
     MLTIPDDRNI VELIREREVI ATDAGQVMDT LNGSWSGRGV HFLQVYTLWG QRMKRRTAID
     HENPARYSMG DSGSLLTKYV MMGTRGGGIQ MTNMLILSTI IQASRFKVFG RQFFLPFPTI
     AAPGGHVNMI LTGFPAPNSK LWLSTNYHHI MPQHVKDRGV IKRPAVDQPE HIGARLLSEK
     DTLEVKVTIG GAVAQDAEGN LPTAGDLMKR AHRKLREQTR HDLYKKNKHK FLNWALTADV
     DLGDYSKLAY SRSTQGAAEK AIGTQTAEKH LKKWFDEKAM VRAGLISVAR GAADAPIPEE
     TESLLLNDTM HVGKVRIAWR FDNDIYLKFK HDEKHNILLF NKAMNPVKEY KYRWHPFWSQ
     PKCMKMLLAY TGVHARPNVL TIKDLTAKFS PHRFRADLHA EDIMGLLKKV PTHMQAQALS
     FVGFDSHEIE EMQKHIPKIH LYEDLSELDD YSGMDDSVKC AELDRITEFL QMVMSDATYG
     ILDDPESPEL RSAVHVQFVS LLADEFNVSY ACACSNEIRI RLPIPELSYD V