RDRP_NCBVS
ID RDRP_NCBVS Reviewed; 1925 AA.
AC Q9YRB3;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Methyltransferase/helicase/RNA-directed RNA polymerase;
DE EC=2.1.1.-;
DE EC=2.7.7.48;
DE EC=3.6.4.13;
OS Nudaurelia capensis beta virus (isolate Pine emperor moth/South Africa)
OS (NbetaV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Hepelivirales; Alphatetraviridae; Betatetravirus.
OX NCBI_TaxID=652108;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=10329566; DOI=10.1006/viro.1999.9677;
RA Gordon K.H., Williams M.R., Hendry D.A., Hanzlik T.N.;
RT "Sequence of the genomic RNA of nudaurelia beta virus (Tetraviridae)
RT defines a novel virus genome organization.";
RL Virology 258:42-53(1999).
CC -!- FUNCTION: RNA-dependent RNA polymerase replicates the viral genome.
CC {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC RNA polymerase family. {ECO:0000305}.
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DR EMBL; AF102884; AAC97509.1; -; Genomic_RNA.
DR RefSeq; NP_048059.1; NC_001990.1.
DR PRIDE; Q9YRB3; -.
DR GeneID; 1450472; -.
DR KEGG; vg:1450472; -.
DR Proteomes; UP000000831; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Nucleotidyltransferase; Reference proteome; RNA-directed RNA polymerase;
KW Transferase.
FT CHAIN 1..1925
FT /note="Methyltransferase/helicase/RNA-directed RNA
FT polymerase"
FT /id="PRO_0000402485"
FT DOMAIN 45..252
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT DOMAIN 533..693
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 694..834
FT /note="(+)RNA virus helicase C-terminal"
FT REGION 737..760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1310..1445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1468..1636
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1666..1727
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1806..1847
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1905..1925
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 737..752
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1313..1329
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1331..1356
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1357..1433
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1478..1528
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1543..1564
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1581..1616
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1671..1686
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1700..1727
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1925 AA; 215612 MW; 49A1354A777E9A1A CRC64;
MEDASKQLRV LDAQERAKAA FQLDFIASVE TLEDAQEKYE GMMFRSGTKL PSTHIKLAID
LRVAEKDLRR HVKNVPTVLE IGPSVESVRY AVQTRDKERV HGCTFSDARD NLRHNKIGYE
AHYDRKIGPD AALLAAGIPT DTFCVDGFSN CEYQSPLAIA CHSLYPDGES NSIMDVAKGM
ALHGTHVIYA WMHLPVELLT LTDADNIFEG YSIRFEETGA LPCTKRRKAI FSGYNDFGSA
YVHDAHHWAG WLKHRGVDTP YGFSILIDIQ QRFGMHTKLK ITRGHSSGSI TTVFPLSKLG
LIWVPNIVKI MYPKAKHEPE YIVTDKKKYE GVCVYVGTRV QSSGKSITLA EIVQYIRTRL
TRIILNGTVH EKTWTIAEQD IERLAVSIMF RKNVERAVSE KALMRAQKKC KSAEKQALLP
VWMRRIANWF QDKFQIDEEV VRKRYLECLK AQPWIHADKV VNCETKRYNP TVAEVGPKNH
LLATTGLREL QREIPSANEP QDRGAKAWHS AHADLDIYAE GLRLDSAKEA AAGKQSLAIT
LQQAFQVLGK TKCEGCNNIE IEYWTGPPGS GKSRAAKPRF ADLQGGVLYC APTRTLRDAL
DESVVHPSRV CTYHNALHVA AKESGNRPFD VIVIDEAETT PACYVGTMHH ASPSSRIVCL
GDPHQIGYID FSDRKDDLKP FSIIAAECRT RRFNTTYRCP QDVLNLPIFK TLYPDAISFS
KQLTSIRYLT RARSVTRTRH AQTLTQDQKP HSEPPVTAHE PQARRTDVIV HYAGTLPERA
LLEKVRHINV ALTRHTNALY IRDESEKGEL VPSLMTPPSW STYRCTPVDK QMVPDPVAVE
RENGSSGPCD SHHIGAITIL QELGKLTDTK GVRVFESEAV PTAHRRVVLD GNLDSGPDRY
PMYQFTNLRG TKYTNIKDNQ QALHTLVGRY ARKINSSSRE DAEFDVKRIT ARLKEWIPFR
TAEPEQVDSC FADAMQKIAE RGHGVDDIED FWSNEGQRIS YHLKGQQKVM DPTKLKLGQG
ISAHEKCANI ALSAWVRIIQ DQMSTSEKFI FANGQSDRDT MSIIEARLQE KAREFKSIDI
KEFDTVHNWV SILVFSWRCD RGCPEHLIEY FEKRSKSRTL SSRIGSVDVS FMLDSGAVWT
IARNTLFASG LMLALFVGVD FIAAKGDDVF LAGNNLYLDA ERLRMGSYLA ANNLKIEKTA
VVSFIGFIVS QAAVTADVVR LATRTYGRSY KNADDLAKYK IAIADHCKLF RSPRTRLMTA
INCATLYGTS KECINYLMDA LDAFGHTKMS DLHLDPGFVM RVTPMKVDER VYSGQDGCQR
ADKTREKQPE PGQPGPQQQQ QASTQEAGSK TSPRSRTDYQ PRPDGRTREP REHPGQPRSD
TREGVKASDD GESHGSDIRG MDSRLSRPGR RIQDEPGRRE NSRRRDTSVN MRSISRDRGR
QIPGTEFYDA TAGWRDLAST SDASPVLQAS VVVHHHHQQH GSRSDERRSG CVRERLEQQD
GLDRSDVPKL GASRERVLHG RPDRSADGRT TPDSTGCIRV TRELPSDIER RHSVLQRTHS
RESGSGGDRA VPTGQRTPEG EPGHSSRDHP NGRNVTARRF RAELHIDDDD RGPGRVRGRS
NPATHGVDGA DAGVGAAGVP DCEPDIRRRK HNHHHDHAAT RVGDGNVAIH SQQRDGHRDR
GRGSATVRVR SEFGRLGTES AGHQLNQDST NEHEPNDAGN AQDHSVPTQR NEGLLYAPEG
VPTRVRNDNG DVLWTGAMED TEDNCGRLPP GNWWTPGYHR QQLRDRRCRD DRYVYINRTL
LQGVPTLRSD TGGGEPLGPL RQCDTSEGRR GANSGSNLDR SAPIRIPGTI QRIRGPIRDG
GQDHSPDTSL CAISSRSGEC GDGLHRERDR ECSLEFHLGE AATKSETCWR NRSRSPQSCG
PHREP
