ID   RDRP_NODAM              Reviewed;        1043 AA.
AC   Q9IMM4;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   29-SEP-2021, entry version 64.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            Short=RdRp;
DE            EC=2.7.7.48;
DE   AltName: Full=RNA replicase;
DE            Short=Protein A;
OS   Nodamura virus (strain Mag115) (NoV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes;
OC   Nodamuvirales; Nodaviridae; Alphanodavirus.
OX   NCBI_TaxID=914672;
OH   NCBI_TaxID=7158; Aedes.
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=11457991; DOI=10.1099/0022-1317-82-8-1855;
RA   Johnson K.N., Johnson K.L., Dasgupta R., Gratsch T., Ball L.A.;
RT   "Comparisons among the larger genome segments of six nodaviruses and their
RT   encoded RNA replicases.";
RL   J. Gen. Virol. 82:1855-1866(2001).
RN   [2]
RP   PROTEIN SEQUENCE OF 917-942; 947-955; 960-978 AND 994-1001, AND ALTERNATIVE
RP   INITIATION.
RX   PubMed=12573589; DOI=10.1006/viro.2002.1769;
RA   Johnson K.L., Price B.D., Ball L.A.;
RT   "Recovery of infectivity from cDNA clones of nodamura virus and
RT   identification of small nonstructural proteins.";
RL   Virology 305:436-451(2003).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 2 RNA segments, RNA1 AND RNA2. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=RNA-directed RNA polymerase;
CC         IsoId=Q9IMM4-1; Sequence=Displayed;
CC       Name=B1;
CC         IsoId=Q9IMM4-2; Sequence=VSP_040283;
CC   -!- MISCELLANEOUS: [Isoform B1]: Produced by alternative initiation at Met-
CC       913 of isoform 1. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the nodaviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; AF174533; AAF97860.1; -; Genomic_RNA.
DR   RefSeq; NP_077730.1; NC_002690.1.
DR   GeneID; 962117; -.
DR   KEGG; vg:962117; -.
DR   Proteomes; UP000166289; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043647; Noda_Vmethyltr_dom.
DR   InterPro; IPR001205; RNA-dir_pol_C.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   Pfam; PF19222; Noda_Vmethyltr; 1.
DR   Pfam; PF00680; RdRP_1; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   1: Evidence at protein level;
KW   Alternative initiation; Direct protein sequencing; Nucleotide-binding;
KW   Nucleotidyltransferase; Reference proteome; RNA-directed RNA polymerase;
KW   Transferase; Viral RNA replication.
FT   CHAIN           1..1043
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000222448"
FT   DOMAIN          588..713
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          879..902
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          918..1043
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        885..902
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        918..952
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        968..982
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..912
FT                   /note="Missing (in isoform B1)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_040283"
SQ   SEQUENCE   1043 AA;  115774 MW;  0DF0CBDB5DBF3EBB CRC64;
     MLNYETIING ASSALNIVSR ALGYRVPLAK SLALVAGSCV VYKIIVHRRT LVAFLVIGPY
     ATVVQHRLPM ALQRAIIEYT REDREISLFP QNSIVSAEHA RKADNGHPIS GGTRDVARET
     ISLAIRAAGF RHYEISPARQ SPAEAASHQH YAAADLVRAA TEDKIQDGDV VVAIDIDYYL
     RDIDRYLGRG VPFMAYTFNP VEVAGRDGDS FFRITNNQVT FDVSGGGSWS HEVWDWCAFG
     EFIETRDASW LAWFARAVGL TKSQIHKVHY CRPWPQSPHR ALVWCLPVAS YWRFTFIPTD
     LHTRTLRRVR YQDTSRPGWN SIVSTGSEGL NISLGREGAD HCVTIPKVHY DMLMGLSSAQ
     SLSSRMIGLK YTDPSVLATV AQYYQGKNVE VADADRIGRA INPKVHWPAH VEVDEAEVSA
     RVYASPLVSD ENMMPMIKRW ETLSLSLDRR VTFQRNPKVP GKRLRAYAIE FVDLVVPERG
     VGVPYSLEDT AAMLDKPSQT LAIQQVWETV DMPPRRLIEA FVKNEPTMKA GRIISSFADM
     RFLLRFSSYT LAFRDQVLHA EHNRHWFCPG LTPEQIATKV VDYVSGVEEP SEGDFSNFDG
     TVSEWLQRHV MNAVYLRYFN HRAQRDLRSY TDMLVSCPAR AKRFGFAYDA GVGVKSGSPT
     TCDLNTVCNG FLQYCSIRMT HPELTPIDAF RLIGLAFGDD SLFERRFAKN YAKVSAEVGM
     VLKIERFDPA QGITFLARVY PDPYTSTTSF QDPLRTWRKL HLTTRDPTIP LATAAIDRVE
     GYLVTDGLSP LTGAYCRMVK RVYEAGGAED AAKRRSRKSH SREKPYWLTV GGAWPQDVKD
     VDLMFQCAAA RTGVDLETLR SLDQRLGEIT DVWADITINR DNEPNPYKDT LDLEGPADGR
     VDDRVFQNDK HVMRLRANQV TSSQAGAAGS GDASNDPNAH DRGSQRQQGS ASVLRVPDRA
     APAGVSSDEQ PAHQTASRSS ASRGGAGPGR GGRRRPGPPA KTTAGGARDG NQARAPTSGP
     SKRQAEGRSR SSRGPAGSRG RGK