ID   RDRP_P1AMV              Reviewed;        1385 AA.
AC   Q07518;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=RNA replication protein;
DE   AltName: Full=156 kDa protein;
DE   AltName: Full=ORF1 protein;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.13;
OS   Plantago asiatica mosaic potexvirus (P1AMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=28354;
OH   NCBI_TaxID=197796; Plantago asiatica.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8113746; DOI=10.1099/0022-1317-75-2-259;
RA   Solovyev A.G., Novikov V.K., Merits A., Savenkov E.I., Zelenina D.A.,
RA   Tyulkina L.G., Morozov S.Y.;
RT   "Genome characterization and taxonomy of Plantago asiatica mosaic
RT   potexvirus.";
RL   J. Gen. Virol. 75:259-267(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8477237;
RA   Solovyev A.G., Novikov V.K., Morozov S.I., Kagramanov V.N., Atabekov I.G.;
RT   "Primary structure of the triple block RNA genes of the Plantago asiatica
RT   mosaic virus.";
RL   Dokl. Akad. Nauk 328:625-628(1993).
CC   -!- FUNCTION: RNA replication. The central part of this protein possibly
CC       functions as an ATP-binding helicase (Probable). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the potexvirus/carlavirus RNA replication
CC       protein family. {ECO:0000305}.
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DR   EMBL; Z21647; CAA79761.1; -; Genomic_RNA.
DR   PIR; S34230; S34230.
DR   RefSeq; NP_620836.1; NC_003849.1.
DR   GeneID; 944434; -.
DR   KEGG; vg:944434; -.
DR   Proteomes; UP000009190; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1385
FT                   /note="RNA replication protein"
FT                   /id="PRO_0000222551"
FT   DOMAIN          59..224
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          635..791
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          792..925
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          1164..1271
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          504..540
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          625..644
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        519..540
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         665..672
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1385 AA;  155617 MW;  66C177E44678A94F CRC64;
     MSNVRNVFSQ LTDPSLKAVI QDEAYRVLKR ELAQTRHTNP FAQSPEAADA LEALGINSHP
     YAITSHTHAA AKAIESDLYE VVSHYLPKEN PVSFLFMKPA KLRFFHRGPT HGDHFLNAHV
     EPKDVPRYPQ ETIINRLADI PTQIAFMGDT LHFLSPSFLT ALFAHSPRLQ TLYATLVLPP
     EPSTSRSLYP QIYTLTYSED GFMYIPGGHA GASYFHKYDQ LEWLTVGHLT APGCPTVTAQ
     RLETKGANHL FIFQRGNYIT PERRTFATND QYVTVPHIFL PENYNCRTPI SKTTMMQMFF
     YIKSGKEAQR EGHLGQVQQL ISTKELQHYQ PSEITLLVNY FEFTATLDSH TCFEDVPPKL
     APKTTPAPPL GISRILQFFR GRPSFVKLIK ALDWQPITLE FPVRDVRTTV RGPPGFKNPF
     TQPQPVNPED ADISSRFQDF SRATNSTPKP TVEAVLEASA ESPDHHTQLK VLCSALSEGQ
     LEAIHALGHS RCGGPLLPEL TAKSTPVEQC PDLPPCPTKS QASPASSNPS GPDAQPTAQN
     PETLPWVAWL PKLNALGFEA LEVQHDPSTG EMIMPITDTQ ELPRATLPAH WQGHNLERIL
     STLRSLNRFP TPWSPDRTRA RAFTSDVKNS RTGKALHRES QTWKETQTML TENTQTELAI
     SVIHGAGGSG KSQALQTLLR QDHSLPIEVV LPTNELRLDW LKKLPHNPPE QFRTFERAFV
     SSHSPVVIFD DYGKLPQGYL EAFALTHANL ELVILTGDPR QSTHHESNEE ALISKLPPAT
     TIYSAFSRYY INATLRNSKF LANKLGVYSA NSCPLNVTYG FQPLPGLHLL VPSLMKKAAF
     TDAGHKVSSY AGCQGITAPK VQILLDNDTT MCTKEVLYTA LSRAVDSIHF VNTNAQSSAF
     WEKLEATPYL KTFLSLVRED KITEFQAPAD APKPVTPPVT HFPVEEPATT LDDFKEELRD
     KFDREIFTSD RGHTNCVQTD DPTTQLFSHQ QAKDEALLWE MIRARLKIST PEANIEEFRA
     KKDLGDILWV NYHRAMGLPK EQPDFSEDLW NRCAEEVQNT YLKKTMAQLK GGERRQSPDF
     HEHQILIFLK SQWVKKMEKL GAKEIKPGQT IASFQQHAVM LYGTMARYMR RFRDALSPNN
     IMINCEKTPQ DLSRWVLNYW DFSKPSYAND FTAFDQSQDG AMLQFEILKA KYFNLPEWVI
     EGYLDIKLSP KIFLGTLSIM RLTGEGPTFD ANTECNIAYT HTRFDIPEGT AQIYAGDDCA
     IAQVCPEKPS FALLKNRIAL QAKPAYTPQE RGQWAEFCGY LITPKGLIKD PLKLHASLEL
     AKAQKKAGVR DAITNVVANY SLDAKLAYSL GDDLQDLLSP HQAHLHQVTV RDLVKFGGSP
     FLNSD