RDRP_PRV
ID RDRP_PRV Reviewed; 1014 AA.
AC Q3KSM3;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 25-MAY-2022, entry version 49.
DE RecName: Full=RNA-directed RNA polymerase;
DE Short=RdRp;
DE EC=2.7.7.48;
DE AltName: Full=RNA replicase;
OS Pieris rapae virus.
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes;
OC Nodamuvirales; Nodaviridae; unclassified Nodaviridae.
OX NCBI_TaxID=327392;
OH NCBI_TaxID=64459; Pieris rapae (Small white butterfly) (Artogeia rapae).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Liu C., Zhang J., Yi F., Wang J., Wang X., Hu Y.;
RT "Sequence of the non-structural protein gene encoded by RNA1 of a new alpha
RT noda-like virus isolated from Pieris rapae larvae in China.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC genome composed of 2 RNA segments, RNA1 AND RNA2. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- SIMILARITY: Belongs to the nodaviridae RNA polymerase family.
CC {ECO:0000305}.
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DR EMBL; AY962576; AAY27743.1; -; Genomic_RNA.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR043647; Noda_Vmethyltr_dom.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF19222; Noda_Vmethyltr; 1.
DR Pfam; PF00680; RdRP_1; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 3: Inferred from homology;
KW Nucleotidyltransferase; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication.
FT CHAIN 1..1014
FT /note="RNA-directed RNA polymerase"
FT /id="PRO_0000223688"
FT DOMAIN 618..740
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT REGION 918..1014
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 983..1002
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1014 AA; 114607 MW; 0691D5F6E57F3D12 CRC64;
MSSVIKTIVV KTLQCTVKCV CVGVRQQYGL LRRHPKTIAC ALVGGIIAHD VLHLYAAVYR
VENPWEFVPT EGPRGVIVLR VYNALRFTAA KWRWNCWTIF GKFPFNSEQF SRIMETSTER
KPDLRPLLVP AHETIEVYPC NNIHSHPRSA EFRSSANEYL VRSVRRAGYP PYVVSSSKRD
FCDGNRFFYC PKDFGMQFRN DPISNNHALV FTDVDYYANM NRWLQLFLPT CLYTLVPTRL
NYSNDEFAYR FENNEIVYNV TGGGEYRHSL WDYKGDTVTV VDEYGNLLIF DIEQRRVQGD
EQHRLIWLLP KAKITDPLWI VGPTEWYDKL LVRKTVQQGR LKILWEPIAD DLSIGLHGSN
YSVSLKGELF EAIRTRISCK DSTPYVSDVE RMLREAKHKN FLTDAPILYH CFQDDVIIRP
NVVKTGSFPV TYNAIPKKAP SVTEDPKMPG QVVTTPLVSQ PALFAGKGFN ADKACIEGRI
EKVRNVTKFP IKYVRYAAEF VKLVIPETIA GTGVPNSIGM IREEQDKVAQ RARFDRVAPI
MSTQTENSIK AFIKTEMYAA AKAPRNISTM APEITIQSSA YSLPMAKIFK KIPWYCPGKN
PKEITLRLAE VCLQDPDHEL EEGDYTCMDG TQSPDYSDLL LLPIYMRYFA KQYRGQFKNL
YNQIYKQRAT TSSGVPYNPE MTIRSGSSIT THAGTLNNAF NIYAALRDMG YDETTAWDKV
GAVFGDDSLN ANHQGEFHSY IEHVTKVLGM RYKSNLRERG QPVLFLGRYF VDPITSYDSF
ADPMRTIGKL HATANKNVSV EQGAANKAHG YITTDSKTPI IGAWARRVLE ITKKKFKNGT
GEEQYRCSNA WPQKDEAAIR AAMAAVLEVD EAQLIAQNEA VLKVNSLDQF PVVFDTYYDH
KQLAEVDGQL VGTDLHIKEE DERQPETSID SVPSTGPPPS NGDPNAGGKP TDGPSKGKRK
VKGRRPRTAG DPATLRLTDA GSSRRVKPPR KLRPEAKRGG RPIETLVFKN SSLS