ID   RDRP_PSVJ               Reviewed;         834 AA.
AC   P28727;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   29-SEP-2021, entry version 69.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Peanut stunt virus (strain J) (PSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Cucumovirus.
OX   NCBI_TaxID=12314;
OH   NCBI_TaxID=4045; Apium graveolens (Celery).
OH   NCBI_TaxID=3818; Arachis hypogaea (Peanut).
OH   NCBI_TaxID=53860; Coronilla.
OH   NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH   NCBI_TaxID=3873; Lupinus luteus (European yellow lupine).
OH   NCBI_TaxID=3879; Medicago sativa (Alfalfa).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=3914; Phaseolus angularis (Azuki bean) (Vigna angularis).
OH   NCBI_TaxID=47097; Tephrosia.
OH   NCBI_TaxID=3898; Trifolium.
OH   NCBI_TaxID=3904; Vicia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1545224; DOI=10.1099/0022-1317-73-3-701;
RA   Karasawa A., Nakaho K., Kakutani T., Minobe Y., Ehara Y.;
RT   "Nucleotide sequence analyses of peanut stunt cucumovirus RNAs 1 and 2.";
RL   J. Gen. Virol. 73:701-707(1992).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D11127; BAA01901.1; -; Genomic_RNA.
DR   PIR; JQ1452; WMVXPJ.
DR   PRIDE; P28727; -.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   Transferase; Viral RNA replication.
FT   CHAIN           1..834
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083282"
FT   DOMAIN          498..611
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          774..834
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        787..802
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   834 AA;  93521 MW;  A2999EEFBC02E672 CRC64;
     MATFSLESLL SGAYGVDTPE EVAFIRERDR VFASGVDTAE SVPCSPDVSE SDPCFEHDDI
     PSTSATCPLE RVSFLVEDLN AMSDVEIKTV FENLIRPTYV GCIEVPEFCR NKLTLSVSVA
     RNLISTASRY PRKLKAYSDL VASIYNGADI FDDAPVDETV SDEVPGYIFL PVERPDGYER
     PPLCSDCGLI GYQCACHDLA GRRAAFAEMT ISHTYELESL SGVIDDATLL ANLGSFLLPI
     HCEYSRVDPS LSSFHLTQPH PTDRVSVESL QAVCDTSLPT HVCYDDTYHQ VFVENSDFTT
     DIDHLTLKQS DLIPKADGGN YLKPVLTTGS GSKRLGTTKE VLTAIKKRNA DVPELSGSVD
     LTRISKAVAR RFKESYFNTN ALAQSNFLNV VGNFHAYMQK WAASGLTYDD LPDLHAENLQ
     LYEHMVKSDV KPVVADTLNV DRPVPATITF HAKGITSQFS PLFISLFERF QRCLRGRVVV
     PVGKISSLEM DNFSVLNKHC LEIDLSKFDK SQGELHLMIQ EEILNLLGCP VDISKWWCDF
     HRYSYIKDKR AGVSMPISFQ RRTGDAFTYF GNTLVTMALF SWCYDTDQFD RMLFSGDDSL
     AFSLNPIEGK SDWFTSLFNM EAKNMDPPTP YICSKFLLTD ELGNTFSVPD PLRELQRLGT
     KKIPDGGLDE SYLKAHFMSF VDRLKFLPRM THHTVAQLIT FYEMKYKKSG AECHASLGGF
     AKYSTNFSIY KELYISDQKR LSLINSYSDD NFEVERKPIL KSKKVKRNRK DVECRANSYE
     APKTSGKAEV SAQTSPTKQE GPWAQKSERV EASRTSRDSV SLFPASTGPP PRRR