ID   RDRP_PVX                Reviewed;        1456 AA.
AC   P09395;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=RNA replication protein;
DE   AltName: Full=165 kDa protein;
DE   AltName: Full=ORF1 protein;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.13;
OS   Potato virus X (PVX).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=12183;
OH   NCBI_TaxID=3711; Brassica campestris (Field mustard).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3205733; DOI=10.1093/nar/16.22.10929;
RA   Skryabin K.G., Kraev A.S., Morozov S.Y., Rozanov M.N., Chernov B.K.,
RA   Lukasheva L.I., Atabekov J.G.;
RT   "The nucleotide sequence of potato virus X RNA.";
RL   Nucleic Acids Res. 16:10929-10930(1988).
CC   -!- FUNCTION: RNA replication. The central part of this protein possibly
CC       functions as an ATP-binding helicase (Probable). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the potexvirus/carlavirus RNA replication
CC       protein family. {ECO:0000305}.
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DR   EMBL; M72416; AAA47167.1; -; Genomic_RNA.
DR   Proteomes; UP000006841; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1456
FT                   /note="RNA replication protein"
FT                   /id="PRO_0000222553"
FT   DOMAIN          59..224
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          695..862
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          863..997
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          1236..1343
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          537..573
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        558..572
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         735..742
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1456 AA;  165407 MW;  F09FDF47E298988C CRC64;
     MAKVREVYQS FTDSTTKTLI QDEAYRNIRP IMEKHKLANP YAQTVEAAND LEGFGIATNP
     YSIELHTHAA AKTIENKLLE VLGSILPQEP VTFMFLKPRK LNYMRRNPRI KDIFHNVAIE
     PRDVARYPKE TIIDKLTEIT TDTAYIGDTL HFLDPSYIVE TFQNCPKLQT LYATLVLPVE
     AAFKMESTHP NIYSLKYFGD GFQYIPGNHG GGAYHHEFSH LQWLKVGKIK WRDPKDSFLG
     HLNYTTEQVE MHTVTVQLQE SFAANHLYCI RRGDLLTPEV RTFGQPDRYV IPPQIFLPKV
     HNCKKPILKK TMMQLFLYVR TVKVAKNCDI FAKVRQLIKS SDLDKYSAVE LVYLVSYMEF
     LADLQATTCF SDTLSGGLLT KTLAPVRAWI QEKKMQLFGL EDYAKLVKAV DFHPVDFSFK
     VETWDFRFHP LQAWKAFRPR EVSDVEEMEN LFSDGDLLDC FTRMPAYAVN AEEDLAAIRK
     TPEMDAGQEV KEPAGDRNQY SNPAETFLSK LHRKHSREVK HQAAKKAKRL AEIQESMRAE
     GEAESNEMSG GMGAIPSNAE LPSTSGARQE LTLPTTKPVP ARWEDASFTD SSVEEEQVKL
     PGKEAVETAT QQVIEGLPWK HWIPQLNAVG FKALEIQRDR SGTMIMPITE MVSGLEKEDF
     PEGTPKELAR ELLAMNRSPA TIPLDLLRAR DYGSDVKNKR IGAITKTQAA SWGEYLTGKI
     ESLTERKVAA CVIHGAGGSG KSHAIQKALR EIGKGSDITV VLPTNELRLD WSKKVPNTEP
     YMFKTYEKAL IGGTGSIVIF DDYSKLPPGY IEALICFYSK IKLVILTGDS RQSVYHETAE
     DASIRHLGPA TEYFSKYCRY YLNATHRNKK DLANMLGVYS ERTGVTEISM SAEFLEGIPT
     LVPSDEKRRL YMGTGRNDTF TYAGCQGLTK PKVQIVLDHN TQVCSANVMY TALSRATDRI
     HFVNTSANSS AFWEKLDSTP YLKTFLSVVR EQALKEYEPA EAEPIREPEP QTHMCVENEE
     SVLEEYKEEL LEKFDREIHS ESHGHSNCVQ TEDTTIQLFS HQQAKDETLL WATIDARLKT
     SNQETNFREF LSKKDIGDVL FLNYQKAMGL PKERIPFSQE VWEACAHEVQ SKYLSKSKCN
     LINGTVRQSP DFDENKIMVF LKSQWVTKVE KLGLPKIKPG QTIAAFYQQT VMLFGTMARY
     MRWFRQAFQP KEVFINCETT PEDMSAWALN NWNFSRPSLA NDYTAFDQSQ DGAMLQFEVL
     KAKHHCIPEE IIQAYIDIKT NAHIFLGTLS IMRLTGEGPT FDANTECNIA YTHTKFDIPA
     GTAQVYAGDD SALDCVPEVK HSFHRLEDKL LLKSKPVITQ QKKGSWPEFC GWLITPKGVM
     KDPIKLHVSL KLAEAKGELK KCQDSYEIDL SYAYDHKDSL HDLFDEKQCQ AHTLTCRTLI
     KSGRGTVSLP RLRNFL