ID   RDRP_PVXCP              Reviewed;        1456 AA.
AC   P22591;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=RNA replication protein;
DE   AltName: Full=165 kDa protein;
DE   AltName: Full=ORF1 protein;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.13;
OS   Potato virus X (strain CP) (PVX).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=12184;
OH   NCBI_TaxID=3711; Brassica campestris (Field mustard).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2392880; DOI=10.1016/0168-1702(90)90054-f;
RA   Orman B.E., Celnik R.M., Mandel A.M., Torres H.N., Mentaberry A.N.;
RT   "Complete cDNA sequence of a South American isolate of potato virus X.";
RL   Virus Res. 16:293-305(1990).
CC   -!- FUNCTION: RNA replication. The central part of this protein possibly
CC       functions as an ATP-binding helicase (Probable). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the potexvirus/carlavirus RNA replication
CC       protein family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M31541; AAA47178.1; -; Genomic_RNA.
DR   EMBL; X55802; CAA39324.1; -; Genomic_RNA.
DR   PIR; S14005; S14005.
DR   Proteomes; UP000008615; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1456
FT                   /note="RNA replication protein"
FT                   /id="PRO_0000222554"
FT   DOMAIN          59..224
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          695..862
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          863..997
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          1236..1343
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          474..499
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          513..563
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          581..600
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        525..548
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        582..596
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         735..742
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1456 AA;  165301 MW;  489BA57EA070BD2E CRC64;
     MAKVREVYQS FTDSTTKTLI QDEAYRNIRP IMEKHKLSNP YAQTIEAAND LEGFGIATNP
     YSIELHTHAA AKTIENKLLE VLGSLLPQEP VTFMFLKPRK LNFMRRNPRI KDIFHNVAIE
     PRDVARYPKE TIIHKLAEIE TDTAYISDTL HFLDPSYIVE TFQNCPKLQT LYATLVLPSE
     AAFKMESTHP NIYSLKYFGD GFQYIPGNHG GGAYHHEFTD LQWLKVGKIK WRDPKDGLLG
     HLNYTHEQVD THTVTVQLQE SFAANHLYCI RRGNMMTPEV RTFGQPDRYV LPPQIFLPKV
     HNCKKPILKK TMMQLFLYVR TVKVAKNCDI FAKVRQLIKS SDRDKFSAVE LVYLVSYMEF
     LAALQATTCF SDTLSGGLLT KTLAPVRAWI QEKKMQLCGL EDYVKLVKAV DWRPVDFSFK
     VETWDFRFTQ LGMWKAFQPS ELSDVEEMNN FFDDGDLLDC FTRMPAYAVN AEEDLAGMRG
     NNQEETSTAP REPEGEKKEY INPAETFLDK LTRKHNRETR SRAAKKAKRL AEIQDSMNRD
     RTEEGSHKTP NMGEAPSNAD LPGVNEVEAG TTFPTLKALP QKWEDASSTD SSTIDPTEII
     PGEEDDKAAT QKVVVGLPWK HWLPQLNAVG FKALEIQRDR NGTMIMPITE MVFGLDKEEF
     PEGTPEALAR ELKAMNRSPT TIPLDLLRAR DYGSDVKNKR IGAITKTQAA SWGEYLTGKI
     ESLPERKVAA CVIHGAGGSG KSHAIQKALR EIGKGSDITV VLPTNELRLD WSKKVPNTEP
     YMFKTYEKAL IGGTGSIVIF DDYSKLPPGY IEALVSFSTK IKLIILTGDS RQSVYHETSD
     DASIRHLGPA TEVFAKYCRY YLNATHRNKK DLANMLGVYS ERTGTTEISM SSEFLEGVPT
     LVPSDEKRRL YMGTGRNDTF TYAGCQGLTK PKVQIVLDHN TQVCSANVMY SALSRATDRI
     HFINTSANSS AFWEKLDSTP YLKTFLSVVR EHALKEYEPA EAEPIKEPEP QTHMCVENEE
     SVLEEYKEEL LEKFDREIHS DAHGHSNCVQ TEDTTIQLFS HQEAKDETLL WATIDARLKT
     SNQESNFREF LSKRDIGDVL FLNYQKAMGL PKEPIPFSQE VWEACAHEVQ SKYLSKSKCN
     LINGTVRQSP DFDENKIMVF LKSQWVTKVE KLGLPKIKPG QTIAAFYQQT VMLFGTMARY
     MRWFRQAFQP KEVFINCETT PEDMSAWALS NWNFTRPSLA NDYTAFDQSQ DGAMLQFEVL
     KAKHHCIPEE IIQAYIDIKT NAQIFLGTLS IMRLTGEGPT FDANTECNIA FTHTKFDIPA
     GTAQVYAGDD SALDCVPEVK QSFHRLEDKL LLKSKPVITQ QKKGSWPEFC GWLITPKGVM
     KDPIKLHVSL KLAEAKGELR KCQDSYEIDL SYAYDHKDSL HDLFDEKQCQ AHTLTCRTLI
     KSGRGTVSLP RLKNFL