ID   RDRP_PVXX3              Reviewed;        1456 AA.
AC   P17779;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=RNA replication protein;
DE   AltName: Full=165 kDa protein;
DE   AltName: Full=ORF1 protein;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.13;
OS   Potato virus X (strain X3) (PVX).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=12185;
OH   NCBI_TaxID=3711; Brassica campestris (Field mustard).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3404114; DOI=10.1099/0022-1317-69-8-1789;
RA   Huisman M.J., Linthorst H.J.M., Bol J.F., Cornelissen B.J.C.;
RT   "The complete nucleotide sequence of potato virus X and its homologies at
RT   the amino acid level with various plus-stranded RNA viruses.";
RL   J. Gen. Virol. 69:1789-1798(1988).
CC   -!- FUNCTION: RNA replication. The central part of this protein possibly
CC       functions as an ATP-binding helicase (Probable). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the potexvirus/carlavirus RNA replication
CC       protein family. {ECO:0000305}.
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DR   EMBL; D00344; BAA00249.1; -; Genomic_RNA.
DR   PIR; JA0102; WMWGPV.
DR   RefSeq; YP_002332929.1; NC_011620.1.
DR   PRIDE; P17779; -.
DR   GeneID; 7065759; -.
DR   KEGG; vg:7065759; -.
DR   Proteomes; UP000008687; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039690; P:positive stranded viral RNA replication; IDA:UniProtKB.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1456
FT                   /note="RNA replication protein"
FT                   /id="PRO_0000222556"
FT   DOMAIN          59..224
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          695..862
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          863..997
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          1236..1343
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          536..564
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         735..742
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1456 AA;  165606 MW;  E06E3FF2AB48E97B CRC64;
     MAKVREVYQS FTDSTTKTLI QDEAYRNIRP IMEKHKLANP YAQTVEAAND LEGFGIATNP
     YSIELHTHAA AKTIENKLLE VLGSILPQEP VTFMFLKPRK LNYMRRNPRI KDIFHNVAIE
     PRDVARYPKE TIIDKLTEIT TETAYISDTL HFLDPSYIVE TFQNCPKLQT LYATLVLPVE
     AAFKMESTHP NIYSLKYFGD GFQYIPGNHG GGAYHHEFAH LQWLKVGKIK WRDPKDSFLG
     HLNYTTEQVE MHTVTVQLQE SFAANHLYCI RRGDLLTPEV RTFGQPDRYV IPPQIFLPKV
     HNCKKPILKK TMMQLFLYVR TVKVAKNCDI FAKVRQLIKS SDLDKYSAVE LVYLVSYMEF
     LADLQATTCF SDTLSGGLLT KTLAPVRAWI QEKKMQLFGL EDYAKLVKAV DFHPVDFSFK
     VETWDFRFHP LQAWKAFRPR EVSDVEEMES LFSDGDLLDC FTRMPAYAVN AEEDLATIRK
     TPEMDVGQEV KEPAGDRNQY SNPAETFLNK LHRKHSREVK HQAVKKAKRL AEIQESMRAE
     GEAEPNETSG GMGAIPSNAE LPGTSDARQE LTLPTTKPVP ARWEDASFTD SSVEEEQVRL
     LGEEAVKTAT QQVIEGLPWK HWIPQLNAVG FKALLIQRDR SGTMIMPITE MVSGLEKEDF
     PEGTPKELAR ELLVMNRSPA TIPLDLLRAR DYGSDVKNKR IGAITKTQAT SWGEYLTGKI
     ESLTERKVAT CVIHGAGGSG KSHAIQKALR EIGKGSDITV VLPTNELRLD WSKKVPNTEP
     YMFKTYEKAL IGGTGSIVIF DDYSKLPPGY IEALVCFYSK IKLIILTGDS RQSVYHETAE
     DASIRHLGPA TEYFSKYCRY YLNATHRNKK DLANMLGVYS ERTGVTEISM SAEFLEGIPT
     LVPSDEKRKL YMGTGRNDTF TYAGCQGLTK PKVQIVLDHN TQVCSANVMY TALSRATDRI
     HFVNTSANSS AFWEKLDSTP YLKTFLSVVR EQALREYEPA EAEPIQEPEP QTHMCVENEE
     SVLEEYKEEL LEKFDREIHS ESHGHSNCVQ TEDTTIQLFS HQQAKDETLL WATIDARLKT
     SNQEANFREF LSKKDIGDVL FLNYQKAMGL PKERIPFSQE VWEACAHEVQ SKYLSKSKCN
     LINGTVRQSP DFDENKIMVF LKSQWVTKVE KLGLPKIKPG QTIAAFYQQT VMLFGTMARY
     MRWFRQAFQP KEVFINCETT PEDMSVWALN NWNFSRPSLA NDYTAFDQSQ DGAMLQFEVL
     KAKHHCIPEE IIQAYIDIKT NAQIFLGTLS IMRLTGEGPT FDANTECNIA YTHTKFDIPA
     GTAQVYAGDD SALDCVPEVK HSFHRLEDKL LLKSKPVITQ QKKGSWPEFC GWLITPKGVM
     KDPIKLHVSL KLAEAKGELK KCQDSYEIDL SYAYDHKDSL HDLFDEKQCQ AHTLTCRTLI
     KSGRGTVSLP RLRNFL