ID   RDRP_PZSVT              Reviewed;         687 AA.
AC   Q9DUT2;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   29-SEP-2021, entry version 73.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Pelargonium zonate spot virus (isolate Tomato/Italy/1982) (PZSV)
OS   (Pelargonium zonate spot virus (isolate Tomato)).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Anulavirus.
OX   NCBI_TaxID=650488;
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=tomato;
RX   PubMed=14573820; DOI=10.1099/vir.0.19391-0;
RA   Finetti-Sialer M., Gallitelli D.;
RT   "Complete nucleotide sequence of Pelargonium zonate spot virus and its
RT   relationship with the family Bromoviridae.";
RL   J. Gen. Virol. 84:3143-3151(2003).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; AJ272328; CAC08527.1; -; Genomic_RNA.
DR   RefSeq; NP_619771.1; NC_003650.1.
DR   GeneID; 956569; -.
DR   KEGG; vg:956569; -.
DR   Proteomes; UP000000411; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..687
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000402417"
FT   DOMAIN          427..540
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   687 AA;  78996 MW;  15D7D862ECE12A14 CRC64;
     MAAFTFENFL SGAYTGLPID KFRALGLNTE DYDEQRWEML VKCVDSGLMQ FSCSRDEALV
     LLWNEEELPK DDDDEVPYEV PCWTPDTDAT VIDDVSEWLA EKTSVRDTVV VCSDYDAVSE
     TPVEVLSVEL EEDSEEDTIA DVCLDARRKS FRDYFTIVEE EFVEEDPLIS LNDGNVCPVR
     THEVTSLEKP VMLDVGRRCD RVNLESLQGA INMNLPSHAY FDDTWHQYFV EGSKLDVDFD
     NIRLRQSEVF CDRDLDRYYQ PELFAGASSR RIGTQKEALV AIRKRNADVP ELADSVDVER
     LSESVAKKFL SSYVCDLKPV VGVMEKMRAY HQKWGDKIDP MFLLKEHNLQ RYEHMIKTDV
     KPTVAHSMHV ERAIPATITF HGKSICAGFS PSFTALFDEF QKSLDERVVI PSGPISTIEM
     DFDIRNKYYL EVDLSKFDKS QGLLHLEFQR KILCKIGLPA HLANWWCDFH YKSFISDPRA
     KVSFNCSFQR RTGDAFTFFG NTLVTMAMFS FCYDTRQFEK MLFAGDDSLA ISSSPIVGCS
     DYFVSLFNME AKIMDPGVPY ICSKFLVSDE LGRCFSAPDP IREFQRLGKK KISADNDDAL
     FEQYVGFKDR MSHMRNFSEY EIQQLKIFFN LKYKQSGEVI EDYMGACMFY SDNFKNFKTL
     FTKTCAPLVA ALNKRVKDKP FRLPPSL