ID   RDRP_QRFVE              Reviewed;         777 AA.
AC   D0QX26;
DT   31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   23-FEB-2022, entry version 40.
DE   RecName: Full=RNA-directed RNA polymerase catalytic subunit;
DE            EC=2.7.7.48;
GN   Name=PB1; OrderedLocusNames=Segment 1;
OS   Quaranfil virus (isolate QrfV/Tick/Afghanistan/EG_T_377/1968) (QRFV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Quaranjavirus.
OX   NCBI_TaxID=1559362;
OH   NCBI_TaxID=6939; Ixodidae (hardbacked ticks).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=19726499; DOI=10.1128/jvi.00677-09;
RA   Presti R.M., Zhao G., Beatty W.L., Mihindukulasuriya K.A., da Rosa A.P.,
RA   Popov V.L., Tesh R.B., Virgin H.W., Wang D.;
RT   "Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the
RT   family Orthomyxoviridae.";
RL   J. Virol. 83:11599-11606(2009).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC       replication and transcription of virus RNA segments. The transcription
CC       of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC       methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC       by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC       transcription of viral mRNAs. During virus replication, PB1 initiates
CC       RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC       serves as a template for the production of more vRNAs.
CC       {ECO:0000250|UniProtKB:P03431}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: RNA polymerase is composed of three subunits: PA, PB1 and PB2.
CC       {ECO:0000250|UniProtKB:P03431}.
CC   -!- SIMILARITY: Belongs to the influenza viruses polymerase PB1 family.
CC       {ECO:0000305}.
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DR   EMBL; FJ861695; ACY56282.1; -; Genomic_RNA.
DR   SMR; D0QX26; -.
DR   Proteomes; UP000029941; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR   InterPro; IPR001407; RNA_pol_PB1_influenza.
DR   Pfam; PF00602; Flu_PB1; 1.
DR   PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..777
FT                   /note="RNA-directed RNA polymerase catalytic subunit"
FT                   /id="PRO_0000443068"
FT   DOMAIN          313..512
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   777 AA;  88189 MW;  F0A1A8291E0F4A6F CRC64;
     MERLNSFLVS TLIRDGMSRE EKEKLVGPYP VGIENTGLGA VSFLYKYVNV PPLAVGAPAP
     KTAESVLRSF EYNRLPDNGK GLRPRQYWME TDGPYPYDVT CANFHLSAAQ EMHKSFLREH
     HAVIDKVTEA MYQRLKTTNA DILTKGKQTW DPINKRSVPS AAAFKEITTV LRTYLKLVGF
     SVLDFVEAFH RMLMLPEMVY NRRVNAEKTV RKRKGGVITL EKKAVVVLET VHLTRNEDVR
     ETVMGWATAF CSYLKSKERG KLKRRAIASA NPILRMFLWI VEELHLELGK QEEMVSSTIS
     IGGEEKRAKI IATPDGLSLN EFNIQATEDA TKWNECLAPE NFCLMHEIWW SHSIREEMGL
     PKPPESAEIM RQIFQQAFYL LSHKRIYLGK GHLIHNQTRA ALLQWKEDHE KYMNEKTLEW
     FRKIKEHLDS EGYVKAPFGM LMGMLNAGST TLALPATKWR LQPGMDCKTV RSSDDSMTVF
     SGKTRQLLME NINRFYDNLK LLGINISQKK TRFFQLKFGE YTSAYQDGDF TAQYGVGTAA
     LRPEGSNPPD DFHSVASQTA TSLRSGTVNF VGAQFRLGIG VDNVRRLYKI DRTPGKRQGV
     PDSALVLSDG GPSPWNFSNC HLPELALKWI THEQNPQATR YLERVMNPNN PFTADAAEIT
     SFSRELNTLV ETSLELPRNL FHTLKRFNAT QKSLLRKGDN DFMKSCNLAM QLFEEVIPAS
     LLQVPSGPQP MSQVMADVLR AQASALRSVG VHFTEEEMEE IRGALNTLEH DSNIDFE