RDRP_RBDVR
ID RDRP_RBDVR Reviewed; 1694 AA.
AC Q05983;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 23-FEB-2022, entry version 89.
DE RecName: Full=Methyltransferase/helicase/RNA-directed RNA polymerase;
DE EC=2.1.1.-;
DE EC=2.7.7.48;
DE EC=3.6.4.13;
OS Raspberry bushy dwarf virus (isolate Malling Jewel raspberry/R15) (RBDV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Mayoviridae; Idaeovirus.
OX NCBI_TaxID=652675;
OH NCBI_TaxID=32247; Rubus idaeus (Raspberry).
OH NCBI_TaxID=75079; Rubus occidentalis (Black raspberry).
OH NCBI_TaxID=75100; Rubus ursinus (California blackberry).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=1469359; DOI=10.1099/0022-1317-73-12-3213;
RA Ziegler A., Natsuaki T., Mayo M.A., Jolly C.A., Murant A.F.;
RT "The nucleotide sequence of RNA-1 of raspberry bushy dwarf virus.";
RL J. Gen. Virol. 73:3213-3218(1992).
CC -!- FUNCTION: RNA-dependent RNA polymerase replicates the viral genome.
CC {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC RNA polymerase family. {ECO:0000305}.
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DR EMBL; S51557; AAB24599.1; -; Genomic_RNA.
DR PIR; JQ1896; JQ1896.
DR RefSeq; NP_620465.1; NC_003739.1.
DR SMR; Q05983; -.
DR GeneID; 963857; -.
DR KEGG; vg:963857; -.
DR Proteomes; UP000001102; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 3: Inferred from homology;
KW ATP-binding; Hydrolase; Nucleotide-binding; Nucleotidyltransferase;
KW Reference proteome; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication.
FT CHAIN 1..1694
FT /note="Methyltransferase/helicase/RNA-directed RNA
FT polymerase"
FT /id="PRO_0000402473"
FT DOMAIN 194..444
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT DOMAIN 829..983
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 984..1145
FT /note="(+)RNA virus helicase C-terminal"
FT DOMAIN 1442..1558
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ SEQUENCE 1694 AA; 191100 MW; 831A8BE8BDB4B382 CRC64;
MECLDFSKLW FSTAAGLQQR CYYDCVAWEC LGDDDLQIFI SGLNRLIESV AVSCTGDEDL
DFVVDSCNEF VTGRDLKSFF AADLPVREVS SVGCISHFIP GSVSGLNVSD LLDNQLYGCS
VFSSDFESKL RDIRDAALSD AASGVSQLVS CHFEKDVRHL LAENANSVKI PVPQKLSDDD
MRILRDHFPR YELKFTQNVD GPHNMAAAHR LLETHDLLSN FPADAPILDI GGNWFSHFRY
GRSNVHSCCP MLDLRDNERH THRLTMTESL MSSLRHRYAG TIDLDPDAHL SRKVSDSMKE
FYKRWAVHPK DLIRLYAGIR DGNSSLYCHH KFGVSWNDVL WESERNNCLT VPEPECPFKA
KYAIMVHSGY DLPLKELIGG MVQHGVVELH GTMIADPAML VATSGYIPAL RCNWEKSKGQ
IWFSFRDDST MGYRHDWEVY SKYLTSTVVS CGKHFYVMER DKYRHGVLFY SIIKCSGSLR
KGDHTFFHNA WFHEMYDKYI MKVPLVKVKD LTGDEGSVEC SWREVVMSRK LVDRVIEVCL
RGVKPINFGN CDDAVHMDNL RIIQNHLLSH SQTLVLNGST IIREEAIPFK DFSPVSVTIY
FEILLTRYKE SLSLAWFHAG LGPDFKLGSW VSSLKRVFYR ILGFPANLLK YVLNALFRCR
DKVSDMEFVK PAVEKLTVLE NTYIGKSLMG DCPTLKEYDD SAFFNILENV GNELFNNSST
DSGKPETPEV TMTGNPNAVI AEAISYCRAE VDRIGKKCER ILHAYQATGN CGGYLNDTDN
VGVFDKMTSW VQKPKEFDHE FGWDGSSFIK LSWFGKIPDF VGRYLVVTDG TRVTTNLKFS
RQYATIPATV TPTIKLVDGV TGCGKTTEIV RRYRPGILIL SVCKANVDEI RRKLAAVDSK
FIRTVDSYLL SPSVTGSCDE LFIDEYGLSH PGILLLAIHI SGIRKVTLFG DSEQIPFCNR
LADFPLKYNS VEDVGLNFDR EIRSTTYRCP QDITLSLQKM YKTKPIKTVS TVESSITIKP
IKSEFEIPLP NAFDGPVLYI CMTKHDESLL KLRWAKENIS SEVRTVHAAQ GLSYKNVVYF
RLTRTDNDLY TKRKLPYHLV AISRHTDKIV YCTTKPEDSS DFSLSALKNT IKTSRDLTQE
ASGSESSYAV VFESNSEVTA TKPEVCENVR KAAEMNFPVS SDALYQKEVP IYGAIPDPKG
KASYNPGSVI RAIEELTPGN TSIDTDALDE LVEVGPMSLQ VGSIRWDVSK ISPRLFTNNK
FAVPHLPTGA LLRRNTSSRQ VGLAIEKRNA NVMNSQKYFD LENLANKAVE RFFDFFIDME
KFSKLPTGVL GSSAEQIQTY QNKTGNKVTD PVCVALSPIQ KYKHMIKRDV KFNLTDGAQS
EYTKAATITY HQPEITQVAT AIFGQFKTRL LACRNKFLNI PLEHDNDLSG YLTKYHLGSE
NNTFTEIDFS KFDKSQGEIH QLIQDLILIK FGCDPEFVAL WSTAHRSSSI FDQNVGIGFK
TDFQRRTGDA FTFLGNSLVT AAMLAFVISD PDREKIRYML VGGDDSLICS YGPIQVPLEP
LGDIFNMSCK LVQPACPYFA SRYLIRRGDE ILCVPDPYKL LVKLGRKDVP DNQASLCEIR
TGLADSAKYI FDDIVKQKLA ILVQVRYNKA APSLYDALCT VHWALSSNTN FSKFYTVTNT
SNEVRRNRRG VKIT
