ID   RDRP_RDVF               Reviewed;        1444 AA.
AC   Q98631;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   23-FEB-2022, entry version 74.
DE   RecName: Full=RNA-directed RNA polymerase P1;
DE            EC=2.7.7.48;
DE   AltName: Full=Replicase;
GN   Name=S1;
OS   Rice dwarf virus (isolate Fujian) (RDV).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Phytoreovirus.
OX   NCBI_TaxID=142804;
OH   NCBI_TaxID=114194; Alopecurus aequalis.
OH   NCBI_TaxID=90397; Echinochloa crus-galli (Barnyard grass) (Panicum crus-galli).
OH   NCBI_TaxID=94400; Nephotettix cincticeps (Green rice leafhopper) (Selenocephalus cincticeps).
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
OH   NCBI_TaxID=147271; Paspalum.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=12549398;
RA   Xiao J., Li Y., Zhang J., Liu J., Chen Z.;
RT   "Nucleotide and protein sequence analysis of rice dwarf virus replicase.";
RL   Wei Sheng Wu Xue Bao 38:348-358(1998).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=16432031; DOI=10.1099/vir.0.81425-0;
RA   Wei T., Shimizu T., Hagiwara K., Kikuchi A., Moriyasu Y., Suzuki N.,
RA   Chen H., Omura T.;
RT   "Pns12 protein of Rice dwarf virus is essential for formation of viroplasms
RT   and nucleation of viral-assembly complexes.";
RL   J. Gen. Virol. 87:429-438(2006).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=20190042; DOI=10.1093/jb/mvq017;
RA   Miyazaki N., Wu B., Hagiwara K., Wang C.Y., Xing L., Hammar L.,
RA   Higashiura A., Tsukihara T., Nakagawa A., Omura T., Cheng R.H.;
RT   "The functional organization of the internal components of Rice dwarf
RT   virus.";
RL   J. Biochem. 147:843-850(2010).
CC   -!- FUNCTION: RNA-directed RNA polymerase that is involved in both
CC       transcription and genome replication. Together with the capping enzyme
CC       P5 and protein P7, forms an enzyme complex positioned near the channels
CC       situated at each of the five-fold vertices of the core.
CC       {ECO:0000255|PROSITE-ProRule:PRU00539, ECO:0000269|PubMed:20190042}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:20190042}. Host
CC       cytoplasm {ECO:0000269|PubMed:16432031}. Note=Located inside the inner
CC       capsid (PubMed:20190042). Found in the interior of spherical
CC       cytoplasmic structures, called virus factories, that appear early after
CC       infection and are the site of viral replication and packaging.
CC       {ECO:0000269|PubMed:20190042, ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the reoviridae RNA-directed RNA polymerase
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U73201; AAB18743.1; -; Genomic_RNA.
DR   RefSeq; NP_620544.1; NC_003773.1.
DR   PRIDE; Q98631; -.
DR   GeneID; 956505; -.
DR   KEGG; vg:956505; -.
DR   Proteomes; UP000002239; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007097; RNA-dir_pol_reovirus.
DR   InterPro; IPR014383; RNA-dir_poll_phytoreovirus.
DR   PIRSF; PIRSF000822; RdRPol_RDV; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50523; RDRP_DSRNA_REO; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Nucleotide-binding; Nucleotidyltransferase;
KW   Reference proteome; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication; Virion.
FT   CHAIN           1..1444
FT                   /note="RNA-directed RNA polymerase P1"
FT                   /id="PRO_0000222778"
FT   DOMAIN          690..897
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          156..182
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        156..170
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1444 AA;  164422 MW;  A0CF28E1CB945D4C CRC64;
     MDLARSDIIP HLLCLFQEII QANIQKVSEA YDLELKIMNI LTLWRNGSDN LISDNDDYMK
     KGLFFSRSND PLALQARYAR MYDDLFKLNN YEVPDDVVRR HDNKILDIIL KESSVPFWYD
     ISDDEAHESM LPEFRLQDIH EFRLNLKRVR VVPDESEEIQ MDESQSDKRR RKKRMEKSRP
     VWLSGSESDR RIELNDSLKP SQKFETKLSS YLLNRLMNEM NPHYCGHPLP ALFVTLIMLK
     AYSIKNKFFS YGIRYMELVC NEIAGPDLNT RTFPVLFGSD GSFVGTRVYS HYPIKLRMIL
     NDLTYLLTYS DLHKFQEFEL DVNDEVLLHM LRTPNDGRQL KKAVTRLNHY YGLKFNPKTT
     DCGVVNGMDF THKHPITKTA DFTSPVLPMT NSFNKAEICY GHNSKILNRA VFTDTVRGHI
     REDLKNVADL DLPKLYEHVS KLVDMRVNYT IIYDLMFLRV MLNLGGYSRS NQITDFRKTI
     DEITKMNEGF LSGADPEKNI DTLNAWMAPT MEDCGYRLTK SILFGKFRKA KYPSDLEAKS
     NIDYYVTARS AGIGNLRISI ETDKRKYKVR TTSKSAFVNA MGSGILDVNP VSNEPMMLTD
     YLLTQTPETR ANLEAAIDSG SKSDSELMRI LGQNSIGSRS TTAWRPVRPI YINVLQAHLA
     QAFIIGPHIN ATVNQHEYQP TSLWFTGDDL GVGFATLYQS GTADIIVPAI EASSTGKALS
     VLADCSSWDQ TYLTATMIPY YNGIKRALLE YQQADMRNFY MIDSGRTGVP GMKLSEIVDW
     FNSFQTKRIF NASYLKERHS FVVKYMWSGR LDTFFMNSVQ NALITRRIAE EVSLRVSNTG
     LSWFQVAGDD AIMVYDGSSI STTEQVTRIN EITVRNYEES NHIINPQKTV ISHISGEYAK
     IYYYAGMHFR DPSIQLHESE KDSGASDVTE SLREFGQVIY EYNKRAIGTL RVNALYGRLI
     AGLAYSVNCP QYDASKRTYA NMKYYPPPTS VIAPAAFKGG LGLSFTGLSL NEVLFIKLHL
     HEAVSQGLHV ISMISFEANE VVSNSLSAYY LKDQKDLLRD MKLGKHLEKV KGISFKSSDL
     AFSGSDFSQG LNLKRESIDK VKLEVSRKSI RDLRSSGISV PSTHAYENLP YASLHQSFKS
     LKVDRDTSKF TNERLLVSLL EYKSDIPRVS VTSQYPVYDL INISKVDELN VRSGGPVRFI
     SAPIEGKLLE ENIGTRQGVQ FKNRGYGGSQ EVLHFIRSNG LVITEQALID LIIKSGVLLM
     INPQRGLIDL FQSLSGDTAS SMHLANFFMA EKPHWEDNAI SLTIAGSLLE NCDSRIENVK
     NFVSVLATGM QKDLQRMFYY VGFVYYAQRL IWSGGHSSKI FVSIDEDKLA DFLRGSKPIT
     RRRKAMAGTK REPINLSANF SYEISEPDRE ISEYDPLILC HPLSMPFFGN WQEKYSVMQS
     DEQM