ID   RDRP_RGDV               Reviewed;        1458 AA.
AC   A4PBP6;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   29-SEP-2021, entry version 36.
DE   RecName: Full=RNA-directed RNA polymerase P1;
DE            EC=2.7.7.48;
DE   AltName: Full=Replicase;
OS   Rice gall dwarf virus (RGDV).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Phytoreovirus.
OX   NCBI_TaxID=10986;
OH   NCBI_TaxID=94400; Nephotettix cincticeps (Green rice leafhopper) (Selenocephalus cincticeps).
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=17426918; DOI=10.1007/s00705-007-0948-7;
RA   Moriyasu Y., Maruyama-Funatsuki W., Kikuchi A., Ichimi K., Zhong B.,
RA   Yan J., Zhu Y., Suga H., Watanabe Y., Ichiki-Uehara T., Shimizu T.,
RA   Hagiwara K., Kamiunten H., Akutsu K., Omura T.;
RT   "Molecular analysis of the genome segments S1, S4, S6, S7 and S12 of a Rice
RT   gall dwarf virus isolate from Thailand; completion of the genomic
RT   sequence.";
RL   Arch. Virol. 152:1315-1322(2007).
CC   -!- FUNCTION: RNA-directed RNA polymerase that is involved in both
CC       transcription and genome replication. Together with the capping enzyme
CC       P5 and protein P7, forms an enzyme complex positioned near the channels
CC       situated at each of the five-fold vertices of the core (By similarity).
CC       {ECO:0000255|PROSITE-ProRule:PRU00539}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Note=Located inside the inner capsid. Found in the
CC       interior of spherical cytoplasmic structures, called virus factories,
CC       that appear early after infection and are the site of viral replication
CC       and packaging. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the reoviridae RNA-directed RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; AB254451; BAF49639.1; -; Genomic_RNA.
DR   RefSeq; YP_001111373.1; NC_009248.1.
DR   GeneID; 4955111; -.
DR   KEGG; vg:4955111; -.
DR   Proteomes; UP000006720; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007097; RNA-dir_pol_reovirus.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50523; RDRP_DSRNA_REO; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Nucleotide-binding; Nucleotidyltransferase;
KW   Reference proteome; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication; Virion.
FT   CHAIN           1..1458
FT                   /note="RNA-directed RNA polymerase P1"
FT                   /id="PRO_0000402402"
FT   DOMAIN          686..894
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          148..168
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          971..993
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1458 AA;  166032 MW;  82961CC32EA46B44 CRC64;
     MSGADVESYI FPRIREILTG GISGIREAYI NELRVCEKLI QWRNGSDNQV DSTNDASVKG
     MVFHRVSERN ELQNRYAGLY DDLFKLNNNN VDIDVITKHD ETIDKIISKE LKSKMWYELN
     DEDIHSSLLP EFQIQTLDDY YDNMKKYLSQ DDRDEKGNDN REEEDVKNRN DNVTRIQWQT
     LMEKDRKVKL HDDLSFSNEV ETSLSTYINL RTKEEMDPRY VYHPVPALFI TLTLLKVLTG
     GKAFSYGVRY LEKLCKTISK GERSLATYPA IFGSNGELVA TRLYSHYAIK MRLILNNMTY
     LLTYKSCHEF KDFYIDVNDE VLLYMLENPN GGRDLKKAVT RLNLYYGLRY NPKTTDSLKI
     IDGVDYHHEH PKYSDRSYDA PIIEPENHFS ASEQCYEHNA KLLNQAVYSK TVKEYIDSDI
     KKVKDLNLPL LTKFTEKLVD MRCNKSIIYD IVFMRTLLNM GGYSRSNQIT DFKGTIDDIT
     KMNDEYLSDV SEGGKRAKMS EWMYPKMEAC GYGLTKSILN GQMVGVSYPS ASESKAHIES
     YITPNSAGIG NLRFDVDVGG KMYKVRTTSK SAFVNALGTN IFNIDTISME PMFLSEYLTH
     LNQDEKMLLY DRRKNRQIQD IELMRLCGQN VIGSRSTTAW RPVRPIYINV IQAHLAQAFI
     IGPHINATVG RQRTPPKGLW FTGEDVGIGF ATIYQNGTSD VISHAIEASA SGRCLSVLAD
     CSSWDQTFLT ESIIPYYQGI KRAISEFGQA DSENYYMYDN QRKDVVGMRL TETIDWFNEY
     QKKRIFNASY LGERYSFKVQ YMWSGRLDTF FMNSVQNALI TERIANQVSS SVAGSPSLVW
     FQVAGDDAIM VYNANSITSS DQVDAIRKTV VDEYTADNHI INPQKTVISH ISGEYAKIYY
     YAGMHFRDPS IQLHESEKIS KATNITEVMR GYAQVAFEYN KRAIGSLRIN SLYARLLASL
     AYSINVKRSD DESIENKPNR RGSKAKARST KTNNLRSFSS VKYYPPLTSV ITPTGVKGGL
     GMSLSGISLN EILMIKELLL DLVEAGLKIV DQVSFEQNEI VSKSLMRHFL RDRKDLIKEM
     GLDKGTKPVM AVRYRSSDSA FSSGNFENGI SMRLESLDSH KLSIARQSEA KLKSVGVNLP
     KMYTYENLPY STINQSLKGI TIDKDLSRMT NSDLVDQLRS IPESECKGNL ISKYPVYGLF
     NARRIISMDE KVDNPIRYIS TPDEGKALER LIGSRTGIQF KNQGYGGSPA IVRFIRRNGL
     TITEENLIDL VISSGAISLA NPKQNMIELF QAISGDQQSS LELANFFMNE KPHWEDKAIS
     ITINGSILEN CDSRISNISR FVSIDAIRIP GDIKKMFTYI AYVYMCQMFI LDENTPSKIH
     ISINEEQLRD FLISAKPISK NRMKQTIDMK FSNQNSGVTI EIDEESLSRT ADLDYEVLKL
     VHPLSVPFLR NLSEGSPM