ID   RDRP_RRSVT              Reviewed;        1255 AA.
AC   O92604;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   23-FEB-2022, entry version 55.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
OS   Rice ragged stunt virus (isolate Thailand) (RRSV).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Spinareovirinae; Oryzavirus.
OX   NCBI_TaxID=649603;
OH   NCBI_TaxID=4534; Oryza latifolia.
OH   NCBI_TaxID=4536; Oryza nivara (Indian wild rice) (Oryza sativa f. spontanea).
OH   NCBI_TaxID=4529; Oryza rufipogon (Brownbeard rice) (Asian wild rice).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9787664; DOI=10.1007/s007050050419;
RA   Upadhyaya N.M., Ramm K., Gellatly J.A., Li Z., Kositratana W.,
RA   Waterhouse P.M.;
RT   "Rice ragged stunt oryzavirus genome segment S4 could encode an RNA
RT   dependent RNA polymerase and a second protein of unknown function.";
RL   Arch. Virol. 143:1815-1822(1998).
CC   -!- FUNCTION: RNA-directed RNA polymerase that is involved in transcription
CC       and genome replication. Following infection, it catalyzes the synthesis
CC       of fully conservative plus strands. After core assembly, which consists
CC       in recruitment of one capped plus-strand for each genomic segments and
CC       polymerase complexes, the polymerase switches mode and catalyzes the
CC       synthesis of complementary minus-strands (By similarity).
CC       {ECO:0000255|PROSITE-ProRule:PRU00539}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
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DR   EMBL; U66714; AAC36456.1; -; Genomic_RNA.
DR   RefSeq; NP_620541.1; NC_003771.1.
DR   SMR; O92604; -.
DR   GeneID; 991204; -.
DR   KEGG; vg:991204; -.
DR   Proteomes; UP000000348; Genome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007097; RNA-dir_pol_reovirus.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50523; RDRP_DSRNA_REO; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication; Virion.
FT   CHAIN           1..1255
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000403633"
FT   DOMAIN          548..756
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   1255 AA;  141380 MW;  B33F3FA497643169 CRC64;
     MTLLVITEQT IHSLCLDHGE TNQIIAEIKQ LEKPELLFSY ITDAEPLATG EVFVGPDICG
     NCITHTFRVP DYVAKPPPYD SKRVYYPYSY TCLGFDSHPY DYLLTLDTKS IFQAIRKITL
     TRKLSIATQS DLDLIILKKL TTQSNCQSRV SSIWRQCVAA CLAFEPQIQN NNSTQSPNLN
     NLVKRMFATL LKPIGRLPFY ERRRNFVWEE EVSCPTILPL LLYSIQNLVT QFCAGVINRM
     EMILAFQYYL DCGVTAYQDE KLRLQKLIRN WLREGLAKFS KIAMPNWTVS GVISCTTVKP
     IMITTHEARE IKSAREAREA SVPTYLLQYI NHAIHKAISP YQTHQYWQAT RVFANDGTYF
     ASVAALTLNK AVRPRIEETT VKYPNSRYLT MNKEHIAVSL PNETDTNFGL ACQFVKQFIP
     RLVTRLKNTN FQEEFIAFLT SSSSGKDFED AAIATMTRTI QRVAKKRIVA AGLESRSYLN
     QQFIADECLA AAKLVGRTQI GRRQRAIAGV NNTRSLLGFP PMLMLKALLD MTGTTSSGKQ
     MGNYLDLLIP LGLSPYANVI FNSADVDAMD ASVQASVQQI MWHFVVYIAL QLERTDYFAF
     TSGDEVIYEL VEGKDSVATS IHMSGLARSC LRAMHLLQPQ NCVLNDDIVG ELVTREPTFP
     SGQPFTTVHH TFTLSNAIMG GTLRVTNLTN QPSTLLNLTV QGDDTRTINY GPKGCIEKCI
     DDQSSFVSDW GFKVSNETSS HTSEYLQQRV SCGTFVGYPD RVSLFAAERP KEGKTMKEKM
     SEIWSLVTDL GCRSRDPQRL VRLMYAIGVA CCCRLTIRTE RLVAEEFIAS EVGRACCAEI
     LVPKVAARAD RGVMLRYHYP ISALWLEEGG QLPPLATKRR DGTWTCFPSY YFQRGDSNRY
     WYWDVSLTEE KWITTVEMRS DWDSNNAIEL IDDEILEAYA TRYALAALGL NKTRQMERLR
     LSEESTIFDV EQLASGLNAY RNLSKIAISH RASERLKNAG VELPDSVLYS RHTQSRIQDA
     ILKAQMTEKE EIEMSLYFFK DKGYLRSLRE LVKSKSGDLA MIHRCEWGER VTILESSIFN
     VVSTTPLSME VYPGSIMSFI LCYTGLKGAH SGQLAGLVGL LQGKYGGGKI SELQFSIAKK
     IYLTKPYLLN EFIIACGLGG EAELALKNAL HAFEMLRGVE FSTVHTPRQF FFGVDSGVTL
     GNHLDYPNGF PGTRIELAAH LLLAMNFLSS NAQSCTGRSI RVRVPRGMWS RCTNF