RDRP_SCV20
ID RDRP_SCV20 Reviewed; 829 AA.
AC P25328; Q90141;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE AltName: Full=p91;
OS Saccharomyces 20S RNA narnavirus (ScNV-20S).
OC Viruses; Riboviria; Orthornavirae; Lenarviricota; Amabiliviricetes;
OC Wolframvirales; Narnaviridae; Narnavirus.
OX NCBI_TaxID=186772;
OH NCBI_TaxID=4932; Saccharomyces cerevisiae (Baker's yeast).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2061340; DOI=10.1016/s0021-9258(18)98966-0;
RA Rodriguez-Cousino N., Esteban L.M., Esteban R.;
RT "Molecular cloning and characterization of W double-stranded RNA, a linear
RT molecule present in Saccharomyces cerevisiae.";
RL J. Biol. Chem. 266:12772-12778(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=1648104; DOI=10.1016/s0021-9258(18)98967-2;
RA Matsumoto Y., Wickner R.B.;
RT "Yeast 20 S RNA replicon. Replication intermediates and encoded putative
RT RNA polymerase.";
RL J. Biol. Chem. 266:12779-12783(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=9685388; DOI=10.1074/jbc.273.32.20363;
RA Rodriguez-Cousino N., Solorzano A., Fujimura T., Esteban R.;
RT "Yeast positive-stranded virus-like RNA replicons. 20 S and 23 S RNA
RT terminal nucleotide sequences and 3' end secondary structures resemble
RT those of RNA coliphages.";
RL J. Biol. Chem. 273:20363-20371(1998).
RN [4]
RP SUBUNIT.
RX PubMed=7650026; DOI=10.1074/jbc.270.34.20084;
RA Garcia-Cuellar M.P., Esteban L.M., Fujimura T., Rodriguez-Cousino N.,
RA Esteban R.;
RT "Yeast viral 20 S RNA is associated with its cognate RNA-dependent RNA
RT polymerase.";
RL J. Biol. Chem. 270:20084-20089(1995).
RN [5]
RP FUNCTION.
RX PubMed=8990396;
RA Garcia-Cuellar M.P., Esteban R., Fujimura T.;
RT "RNA-dependent RNA polymerase activity associated with the yeast viral
RT p91/20S RNA ribonucleoprotein complex.";
RL RNA 3:27-36(1997).
RN [6]
RP SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=10833519; DOI=10.1074/jbc.m002281200;
RA Solorzano A., Rodriguez-Cousino N., Esteban R., Fujimura T.;
RT "Persistent yeast single-stranded RNA viruses exist in vivo as genomic
RT RNA.RNA polymerase complexes in 1:1 stoichiometry.";
RL J. Biol. Chem. 275:26428-26435(2000).
CC -!- FUNCTION: RNA-directed RNA polymerase that replicates the viral (+) and
CC (-) genome. {ECO:0000269|PubMed:8990396}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48;
CC -!- SUBUNIT: Forms a ribonucleoprotein complex with the 20S RNA, where a
CC single polymerase molecule binds to a single viral RNA genome. Since
CC the viral RNA is not encapsidated, ribonucleoprotein complex formation
CC appears to be the strategy to survive in the host as persistent virus.
CC {ECO:0000269|PubMed:10833519, ECO:0000269|PubMed:7650026}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:10833519}.
CC Note=The virus has no extracellular transmission pathway. It exists as
CC a ribonucleoprotein viral particle in the host cytoplasm and can be
CC transmitted through mating or cytoplasmic mixing (cytoduction).
CC -!- MISCELLANEOUS: Yeast strains also contain a double-stranded RNA (dsRNA)
CC called W. The (+)-strand of W is identical to the single-stranded 20S
CC RNA. The W dsRNA seems to be a by-product of the viral RNA replication
CC cycle.
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DR EMBL; AF039063; AAC98925.1; -; mRNA.
DR EMBL; M64034; AAA47824.1; -; Genomic_RNA.
DR PIR; A40894; A40894.
DR RefSeq; NP_660178.1; NC_004051.1.
DR PRIDE; P25328; -.
DR GeneID; 949218; -.
DR KEGG; vg:949218; -.
DR Proteomes; UP000001104; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR SUPFAM; SSF56672; SSF56672; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Nucleotidyltransferase; Reference proteome;
KW Ribonucleoprotein; RNA-binding; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication.
FT CHAIN 1..829
FT /note="RNA-directed RNA polymerase"
FT /id="PRO_0000097459"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 826..829
FT /note="SGFP -> V (in Ref. 2; AAA47824)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 829 AA; 91809 MW; B0B323FC64732170 CRC64;
MKEPVDCRLS TPAGFSGTVP PPGRTKAARP GTIPVRRSRG SASALPGKIY GWSRRQRDRF
AMLLSSFDAA LAAYSGVVVS RGTRSLPPSL RLFRAMTRKW LSVTARGNGV EFAIASAKEF
SAACRAGWIS GTVPDHFFMK WLPEPVRRKS GLWAQLSFIG RSLPEGGDRH EIEALANHKA
ALSSSFEVPA DVLTSLRNYS EDWARRHLAA DPDPSLLCEP CTGNSATFER TRREGGFAQS
ITDLVSSSPT DNLPPLESMP FGPTQGQALP VHVLEVSLSR YHNGSDPKGR VSVVRERGHK
VRVVSAMETH ELVLGHAARR RLFKGLRRER RLRDTLKGDF EATTKAFVGC AGTVISSDMK
SASDLIPLSV ASAIVDGLEA SGRLLPVEIA GLRACTGPQH LVYPDGSEIT TRRGILMGLP
TTWAILNLMH LWCWDSADRQ YRLEGHPFRA TVRSDCRVCG DDLIGVGPDS LLRSYDRNLG
LVGMILSPGK HFRSNRRGVF LERLLEFQTR KTVYEHAVIY RKVGHRRVPV DRSHIPVVTR
VTVLNTIPLK GLVRASVLGR DDPPVWWAAA VAESSLLSDY PRKKIFAAAR TLRPGLSRQF
RRLGIPPFLP RELGGAGLVG PSDRVDAPAF HRKAISSLVW GSDATAAYSF IRMWQGFEGH
PWKTAASQET DTWFADYKVT RPGKMYPDRY GFLDGESLRT KSTMLNSAVY ETFLGPDPDA
THYPSLRIVA SRLAKVRKDL VNRWPSVKPV GKDLGTILEA FEESKLCTLW VTPYDASGYF
DDSLLLMDES VYQRRFRQLV IAGLMREGRM GDLLFPNWLP PSTVVSGFP
