ID   RDRP_SCV23              Reviewed;         941 AA.
AC   Q07048;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 2.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=p104;
OS   Saccharomyces 23S RNA narnavirus (ScNV-23S).
OC   Viruses; Riboviria; Orthornavirae; Lenarviricota; Amabiliviricetes;
OC   Wolframvirales; Narnaviridae; Narnavirus.
OX   NCBI_TaxID=198599;
OH   NCBI_TaxID=4932; Saccharomyces cerevisiae (Baker's yeast).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1587863; DOI=10.1016/s0021-9258(19)50099-0;
RA   Esteban L.M., Rodriguez-Cousino N., Esteban R.;
RT   "T double-stranded RNA (dsRNA) sequence reveals that T and W dsRNAs form a
RT   new RNA family in Saccharomyces cerevisiae.";
RL   J. Biol. Chem. 267:10874-10881(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9685388; DOI=10.1074/jbc.273.32.20363;
RA   Rodriguez-Cousino N., Solorzano A., Fujimura T., Esteban R.;
RT   "Yeast positive-stranded virus-like RNA replicons. 20 S and 23 S RNA
RT   terminal nucleotide sequences and 3' end secondary structures resemble
RT   those of RNA coliphages.";
RL   J. Biol. Chem. 273:20363-20371(1998).
RN   [3]
RP   SUBUNIT.
RX   PubMed=7961969; DOI=10.1016/s0021-9258(18)43948-8;
RA   Esteban L.M., Fujimura T., Garcia-Cuellar M., Esteban R.;
RT   "Association of yeast viral 23 S RNA with its putative RNA-dependent RNA
RT   polymerase.";
RL   J. Biol. Chem. 269:29771-29777(1994).
RN   [4]
RP   SUBCELLULAR LOCATION, AND SUBUNIT.
RX   PubMed=10833519; DOI=10.1074/jbc.m002281200;
RA   Solorzano A., Rodriguez-Cousino N., Esteban R., Fujimura T.;
RT   "Persistent yeast single-stranded RNA viruses exist in vivo as genomic
RT   RNA.RNA polymerase complexes in 1:1 stoichiometry.";
RL   J. Biol. Chem. 275:26428-26435(2000).
CC   -!- FUNCTION: RNA-directed RNA polymerase that replicates the viral (+) and
CC       (-) genome.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- SUBUNIT: Forms a ribonucleoprotein complex with the 23S RNA, where a
CC       single polymerase molecule binds to a single viral RNA genome. Since
CC       the viral RNA is not encapsidated, ribonucleoprotein complex formation
CC       appears to be the strategy to survive in the host as persistent virus.
CC       {ECO:0000269|PubMed:10833519, ECO:0000269|PubMed:7961969}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:10833519}.
CC       Note=The virus has no extracellular transmission pathway. It exists as
CC       a ribonucleoprotein viral particle in the host cytoplasm and can be
CC       transmitted through mating or cytoplasmic mixing (cytoduction).
CC   -!- MISCELLANEOUS: Yeast strains also contain a double-stranded RNA (dsRNA)
CC       called T. The (+)-strand of T is identical to the single-stranded 23S
CC       RNA. The T dsRNA seems to be a by-product of the viral RNA replication
CC       cycle.
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DR   EMBL; U90136; AAC98708.1; -; Genomic_RNA.
DR   PIR; A38149; A38149.
DR   RefSeq; NP_660177.1; NC_004050.1.
DR   GeneID; 949220; -.
DR   KEGG; vg:949220; -.
DR   Proteomes; UP000007190; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   SUPFAM; SSF56672; SSF56672; 1.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Nucleotidyltransferase; Ribonucleoprotein; RNA-binding;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..941
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000283652"
FT   REGION          875..918
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        881..908
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   941 AA;  104297 MW;  7FB9CED5D4442D11 CRC64;
     MHHKVNVKTQ REVHFPMDLL QACGASAPRP VARVSRATDL DRRYRCVLSL PEERARSVGC
     KWSSTRAALR RGLEELGSRE FRRRLRLADD CWRAICAAVC TGRKFPSFSV TDRPARARLA
     KVYRMGRRLL VGVVCRGESV VSDLKQECAD LRRVIFEGST RIPSSSLWGL VGVLGWTSPE
     RAMQLTFIGR ALPYGSPDVE RRALASHAAT LSIPAECHPN YLVAAEQFAK SWADDNLPRK
     FRIYPIAVQE SSCMEYSRAQ GGLLQSFRKG FVGYDPAAPS ADPDDLELAK ERGFSRIRAS
     WYSTFRYRGE LKSTNQSLEA RVAVVPERGF KARIVTTHSA SRVTFGHQFR RYLLQGIRRH
     PALVDVIGGD HRRAVETMDG DFGLLRPDGR LLSADLTSAS DRIPHDLVKA ILRGIFSDPD
     RRPPGTSLAD VFDLVLGPYH LHYPDGSEVT VRQGILMGLP TTWPLLCLIH LFWVELSDWA
     PARPNHSRGF VLGESFRICG DDLIAWWRPE RIALYNQIAV DCGAQFSAGK HLESKTWGIF
     TEKVFTVKPV KMKVRVRSEP SLKGYVFSRS SAFSCRMGGK GITGIRAARL YTIGAMPRWS
     RRIRDVYPGS LEHRTASQRY GEPVTVYRFG RWSSAIPLRW AVRAPTRTVG NPVQSLPDWF
     TVGPAASSVA ADSNAFGAVS RVLRRMFPGL PRKLASAGIP PYLPRVFGGG GLVKSTGLTT
     KIGAVASRRW MSRIGHDLYR SRERKSTLGR VWTLSTSPAY AASLHEVEKF MDRPDIILTR
     KCRNPMLKHA RELGLFEEVF ESRVGGGILW ASLNGKALVE SHSPSILQVS RNLRRSLACP
     SGGFLRPSAP IGKLVQRHTL PRGTVWFLES SATDSARQGG MGLPPPPPPP LGGGGMAGPP
     PPPFMGLRPE SSVPTSVPFT PSMFSERLAA LESLFGRPPP S