ID   RDRP_SJNNV              Reviewed;         983 AA.
AC   Q9QAZ8;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   29-SEP-2021, entry version 78.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            Short=RdRp;
DE            EC=2.7.7.48;
DE   AltName: Full=RNA replicase;
DE            Short=Protein A;
OS   Striped jack nervous necrosis virus (SjNNV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes;
OC   Nodamuvirales; Nodaviridae; Betanodavirus.
OX   NCBI_TaxID=35297;
OH   NCBI_TaxID=13489; Dicentrarchus labrax (European seabass) (Morone labrax).
OH   NCBI_TaxID=215347; Epinephelus akaara (Hong Kong grouper) (Serranus akaara).
OH   NCBI_TaxID=8267; Hippoglossus hippoglossus (Atlantic halibut) (Pleuronectes hippoglossus).
OH   NCBI_TaxID=8187; Lates calcarifer (Barramundi) (Holocentrus calcarifer).
OH   NCBI_TaxID=163134; Oplegnathus fasciatus (Barred knifejaw) (Scaradon fasciatus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10580064; DOI=10.1099/0022-1317-80-11-3019;
RA   Nagai T., Nishizawa T.;
RT   "Sequence of the non-structural protein gene encoded by RNA1 of striped
RT   jack nervous necrosis virus.";
RL   J. Gen. Virol. 80:3019-3022(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=11602777; DOI=10.1099/0022-1317-82-11-2653;
RA   Iwamoto T., Mise K., Mori K., Arimoto M., Nakai T., Okuno T.;
RT   "Establishment of an infectious RNA transcription system for striped jack
RT   nervous necrosis virus, the type species of the betanodaviruses.";
RL   J. Gen. Virol. 82:2653-2662(2001).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 2 RNA segments, RNA1 AND RNA2. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SIMILARITY: Belongs to the nodaviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; AB025018; BAA84210.1; -; Genomic_RNA.
DR   EMBL; AB056571; BAB64329.1; -; Genomic_RNA.
DR   RefSeq; NP_599247.1; NC_003448.1.
DR   GeneID; 991144; -.
DR   KEGG; vg:991144; -.
DR   Proteomes; UP000000414; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043647; Noda_Vmethyltr_dom.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF19222; Noda_Vmethyltr; 1.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..983
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000222450"
FT   DOMAIN          579..701
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          879..983
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        882..897
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        906..944
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   983 AA;  110630 MW;  F76890AA597F8BE1 CRC64;
     MRRFEFELAR MSGAAFCVVT GYRLLTSKWL ADRVEDYRQR VIADRKQILR DAAVIRTSIQ
     KQMELVRISV RKGHSHQEAA TERNSATDTM IGVVEKCGYE PYIISPSPRE KEYHGSRQFY
     SLADFRQDYR RDEITDRHVI VMTDVDYYVD MHELVGLGVP ILLYTFQPST VSGEVKDGYF
     TITDDHVHYR VAGGKDVRHR IWNYNQDTMF VRSKPRGFWA SLKQILRDIT GITALCGYLY
     LKLGIAPFGD QVTLFTVDQF KMGEHRNIVS IVPFATCRSN LLKISEYGAE LDYMRYQQRN
     NNANFNAVTY ISQEGPLISL GLEGNFASVQ LPLQDFENIR TAYELSKNNN LSDTVRRSAR
     SCKEAAIIHK CLQAGCDLAS EVVHKPGELA RHYQALGDTY DIDPSEQGKC YAREYAPGPL
     TQTAVFPSES RSNELATIDG RIAGPQAKAK SREHITPKMH KVARDFVRHL VPTAGLGRPY
     PLTYVEEHQT KPLQRARNDA NRYHDEFTMI VKAFQKKEAY NAPNYPRNIS TVPHTQNVKL
     SSYTYAFKEA VLQHVPWYMP THTPAEIAEA VQSLAASSTE LVETDYSKFD GTFLRFMREN
     VEFAIYKRWV HLDHLTELST LLGNELQAPA VTRLGIKYDP DCSRLSGSAL TTDGNSIANA
     FVSYLAGRQA GMDDDEAWTW IGIVYGDDGL RSGNVSDALL SKTASSLGFD LKIVNRAPRG
     SPVTFLSRVY LDPWSSPASV QSPLRTLLKL HTTCDTQSDI EDVGWAKTQA YLVTDCLTPF
     IGHWCRAYQR NCTARVVQYA DYNDIPFWVK NEDHVGNSWP QSDSVDWNDV VANELGLTTA
     ELLKHLAALD AYTGPVSGLP RLTTSLDLEP KMPVALDGEV QAGPSQQPQT DKDGTSPTGD
     RSAPRRARTA LQDADGRACR SRRSDRSPGK RDANVRDKRQ RRSTTPPRSR PSVPGPSSSG
     RRTDGDRVRG GAARQRQRRR SPV