ID   RDRP_SSDRV              Reviewed;        1700 AA.
AC   Q6YI57;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=RNA replication protein;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.13;
OS   Sclerotinia sclerotiorum debilitation-associated virus (isolate
OS   Sclerotinia/China/Xie/-) (SsDRV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Sclerodarnavirus.
OX   NCBI_TaxID=686987;
OH   NCBI_TaxID=5180; Sclerotinia sclerotiorum (White mold) (Whetzelinia sclerotiorum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=16361437; DOI=10.1099/vir.0.81522-0;
RA   Xie J., Wei D., Jiang D., Fu Y., Li G., Ghabrial S., Peng Y.;
RT   "Characterization of debilitation-associated mycovirus infecting the plant-
RT   pathogenic fungus Sclerotinia sclerotiorum.";
RL   J. Gen. Virol. 87:241-249(2006).
CC   -!- FUNCTION: RNA replication. The protein possibly functions as an ATP-
CC       binding helicase (Probable). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- MISCELLANEOUS: The sclerotinia sclerotiorum debilitation-associated
CC       virus contains only a single ORF.
CC   -!- SIMILARITY: Belongs to the potexvirus/carlavirus RNA replication
CC       protein family. {ECO:0000305}.
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DR   EMBL; AY147260; AAN64332.2; -; Genomic_RNA.
DR   RefSeq; YP_325662.1; NC_007415.1.
DR   GeneID; 5075997; -.
DR   KEGG; vg:5075997; -.
DR   Proteomes; UP000000395; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1700
FT                   /note="RNA replication protein"
FT                   /id="PRO_0000401072"
FT   DOMAIN          299..462
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          842..991
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          992..1128
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          1369..1480
FT                   /note="RdRp catalytic"
FT   BINDING         868..875
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1700 AA;  193148 MW;  959C8A5DB1F64140 CRC64;
     MKLRNPKAPI RNDRKAQRSF HKMLSLKAQA KLALTIQDRL ELGLLSNNFI RKKLQFVDGY
     CYLKFLRCAE RKTAARNLGS DPSLGLMKRK LVPSQYHSWQ SNYTLLVTKS SKLTFAHVNR
     ALGGQKLAEV PANCCIGSTI CPFLSVPGDH SSYLVNQLPY MNGYCYLKLI RRTCRFNAVV
     TLGPWPLATD FFDYIIHRNL NKDLGLFKCN LENTINGSFC HIVEADPGSQ NCIFPLPQDC
     RIGGSISEVV KTLGPDKEMI ERDAVTEIKK EIAIVRKYNP YHHSSKQQSA LESYGIGSDP
     YAVRSHTHAA EKAIENKLLD IVGMNLRRRS VITMLWQKRN KAHLMGRSNC KDVYVNTIME
     AKDLVRYDQF SFGLPSVATS TAFIGDALHH MTPESVFDLF ERSPNLMVLH ATIVIPPETL
     LKCRSSNPEL YSLRYYDDKF VYIPEGHAGG SYVHEVKNSN WLAISHIQRG GKFLTVKRLE
     TLAAHHYFVI VKGKVETDSI RVFQSPSQVE LLDIFADRQS NVRCSLDHAF AIKMERYVHS
     LKRLELADVT AKTRQLLSSE ELLQYSPTDL VKIDNYFYFL AHTSRFNSSE ELIGSGFFES
     LVSPLKQWFS EICEKFLGKS NFHKTLEALE WKVINYDVKT VIYDMSKPWE KLHWKSENNL
     LSFDLDSNDN PTSLVDNTDC EASYSESTKF DYELVQVEDD FIDIRIPGID IPIYDSEYLA
     SEEPVEVEES PIPEVLPEIE DNLSDSMSID GWMSQSLKTF LPEHDDKVLS ILEKFGVSKY
     GQVVGKNLIL PITDFKSVQF EKIGQDEFTE SLQDRGFGFV SYTPDAERVA VAATDLEHGQ
     GVLITSDEAG ELFKKAMPMS VYTCVILGAG GAGKTTFVEK FVKDNPKSFT VVTPLSVLKK
     EWQRKGAKNV FTYETALKRS LKKPANEYVI LDDFTRFPAG WIELYMSLNT KSKYIVIGDS
     RQADSHSMSG AFANALVPAI DLFAPLSPFY LNWTWRMTRP VANALGHVSW KLPESGKPIL
     SVSSEVPKDC VVLAPSTTLK VGVETVNDKA FTYTSAQGAT FDKVAILIDD NITRVCGDKA
     VYTALSRSKG EIVFVSTVTG PDTFEKVKCT PFLRTFVELV REYELNQPKV REPEEDFVDD
     VTPVTSQPKV SEEFLIEELN KNSVEKFDRE IFRADLGHTD AVKEIGRVTE QIPRQQRSDE
     ALNLVTLDKR VHHATVEENL DELARKKALG NILWTNFKEQ YYSGLESVMV DQDLLVSCRA
     EITKTYLSKT EALLKGGQLR QSPDFDKFKI ADFLKTQWVR KTEKYGLPIK AGQTVTSFMQ
     ETVMATGTLS RYMRRMFDKL CTNPNVYLHR EKTEQDFSTW VKNGWNFSGH ATINDCEAFD
     ASQDGAFVEF ERLHAEFLGV PRELIDFYVD TKIKSYIWRG TISVMRLSGE GPTYDFNTWA
     NMAFMATKYS IPSVAMTAYS GDDFACDQVL SVKPAFKELE CRFKLKEKRF LKSQGRGSYA
     DFCGMIITPN GVIKNPRKLY LSLKSHDEIG TIDKAIVNYY NDLRTLISLG DNIFSALDAT
     ETEFFAGCLN VVHDYILRGS NYEGHQNLTL FKKPRTIKWR VERDETRDCL IHMIIQDRKF
     IRNLLEGVEQ TCDNKPNSNL IFRGMTNGYQ KRAKFNQLLN RQDEYLDKVL HKSAGQNALR
     RLTATLDDTQ DSTVAEQSAV