ID   RDRP_TAV                Reviewed;         828 AA.
AC   P29035;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   29-SEP-2021, entry version 75.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Tomato aspermy virus (TAV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Cucumovirus.
OX   NCBI_TaxID=12315;
OH   NCBI_TaxID=4627; Canna.
OH   NCBI_TaxID=41568; Chrysanthemum morifolium (Florist's daisy) (Dendranthema grandiflorum).
OH   NCBI_TaxID=4688; Lilium.
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2016592; DOI=10.1099/0022-1317-72-4-779;
RA   Moriones E., Roossinck M.J., Garcia-Arenal F.;
RT   "Nucleotide sequence of tomato aspermy virus RNA 2.";
RL   J. Gen. Virol. 72:779-783(1991).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D10663; BAA01514.1; -; Genomic_RNA.
DR   RefSeq; NP_620761.1; NC_003838.1.
DR   GeneID; 962653; -.
DR   KEGG; vg:962653; -.
DR   Proteomes; UP000007399; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..828
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083283"
FT   DOMAIN          515..628
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          776..828
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        778..812
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        813..828
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   828 AA;  93511 MW;  5704239CEE168690 CRC64;
     MSTFSLENCL NGSYGVDTPE EVTFIRRRDA NPSAFHGEET VPSNTTEVHS ESVSYSSDFE
     GYSCDSDFTG DNQYSSFDGA DSLLLSYDID EYIDLSESEL RVLFDEMVQP IHFGMVLSPT
     FSRNRFLSCL SLAKSVVVAP LYDPERTLRP FPELVKNLYS GVHDIYLSDS EEAAISSVTD
     TIEGYTFSPR DVVDNYEPPP LCSVCGLIAY QCPHYDINSL RKNCAEMTIA HDYPIEGLCG
     LIDDATLLKN LGSFLLPIQC EYTKLPEPTL ITPPELTRPT DRVTVDLLQA ICDSTLPTHV
     CYDDTYHQTF IENADYSVDI DRVRLKQSDL LAKVIDEGHL KPVLNTGSGQ KRIGTTREVL
     CAIKKRNADV PELCGSVNLK RLSDDVAECF MLSFMNGDKL CSSNFINIVS DFHAYMSKWQ
     SVLSYDDLPD LNAENLQFYE HMVKSDVKPS VTDTLNIDRP LPATITFHRK QLTSQFSPLF
     TALFQRFQRC LTKRVILPVG KISSLEIKDF SVLNKFCLEI DLSKFDKSQG ELHLMIQEGI
     LNRLGCPVHI SKWWCDFHRM SYIKDKRAGV SMPISFQRRT GDAFTYFGNT LVTMSMFAWC
     YDTSQFDKLI FSGDDSLGFS IKAPVGDPSL FTSLFNMEAK VMEPSVPYIC SKFLLTDDFG
     NTFSVPDPLR EIQRLGSKKI PLDEDDRSLH AHFMSFVDRL KFLNHMNQTS MTQLSLFYEM
     KYRKSGDDIL LVLGAFNKYT ANFNAYKELY YSEKQQCALI NSFSISDLIV GRGKSSKASR
     RKAVESNGKH RDPSTRDHSK VGTDESKETS TEETTQTEPQ GAGSQKSK