ID   RDRP_TBSVC              Reviewed;         818 AA.
AC   P15962; Q88485;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p92;
DE   Contains:
DE     RecName: Full=Protein p33;
GN   ORFNames=ORF1;
OS   Tomato bushy stunt virus (strain Cherry) (TBSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus.
OX   NCBI_TaxID=12147;
OH   NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH   NCBI_TaxID=3749; Malus.
OH   NCBI_TaxID=3766; Pyrus (pears).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=4111; Solanum melongena (Eggplant) (Aubergine).
OH   NCBI_TaxID=29777; Tolmiea menziesii.
OH   NCBI_TaxID=13305; Tulipa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2353450; DOI=10.1016/0042-6822(90)90468-7;
RA   Hearne P.Q., Knorr D.A., Hillman B.I., Morris T.J.;
RT   "The complete genome structure and synthesis of infectious RNA from clones
RT   of tomato bushy stunt virus.";
RL   Virology 177:141-151(1990).
CC   -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC       the virus replication. {ECO:0000250|UniProtKB:P11640}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC       297. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the tombusviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; M21958; AAB02534.1; -; Genomic_RNA.
DR   EMBL; M21958; AAB02535.1; -; Genomic_RNA.
DR   PIR; A35315; RRVGCT.
DR   RefSeq; NP_062897.1; NC_001554.1.
DR   RefSeq; NP_062898.1; NC_001554.1.
DR   GeneID; 1493955; -.
DR   GeneID; 1493956; -.
DR   KEGG; vg:1493955; -.
DR   KEGG; vg:1493956; -.
DR   Proteomes; UP000001666; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   InterPro; IPR013707; Tombusvirus_p33.
DR   Pfam; PF00998; RdRP_3; 1.
DR   Pfam; PF08500; Tombus_P33; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA suppression of termination; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..818
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000041336"
FT   CHAIN           1..296
FT                   /note="Protein p33"
FT                   /id="PRO_0000041337"
FT   DOMAIN          524..641
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   818 AA;  92253 MW;  D61AD04D5F94BC42 CRC64;
     METIKRMIWP KKEIFVGDFA TGVNRTVPVN IFQLVCRVVL RYMRTGKIEC DSDSMTKFIV
     ELLKTDCAAK WEWFMKRRQR GDYIVPLSIA SIPIIPLLSY ATRVRAVSVK AFGNELSFNV
     RVPRPSVPKK GLLLRLAAGL ALAPICALAV YATLPREKLS VFKLRTEARA HMEDEREATD
     CLVVEPAREL KGKDGEDLLT GSRLTKVIAS TGRPRRRPYA AKIAQVARAK VGYLKNSPEN
     RLIYQRVMIE IMDKDCVRYV DRDVILPLAI GCCFVYPDGV EESAALWGSQ ESLGVKXGGL
     VRLPGVVTQI NRDIPSDVLL PQEVLEVRTG PPNAKDRNIF MVAGCPSQAR FLVHNHCLKN
     LKRGLVERVF CVERNGKLAR TPQPTKGAFG RLSPFRKAVC EKVGVAHRLG YDGFLSYYSG
     AKLRTYTRAV ESLHITPVSE RDSHLTTFVK AEKISTSKGD PAPRVIQPRN PRYNVELGRY
     LRHMESKLMK AVDGVFGETT CIKGYTADEV GAIFRAKWDR FDKPVAIGLD ASRFDQHCSV
     EALQYEHSFY RAMYPGNKLL GKLLEWQLHN KGKGYVPDGT ITYRKEGCRM SGDINTSLGN
     YLLMCAMVHG YMRHLGINEF SLANCGDDCV LIVERRNLKQ IQRTLPEYFL NLGYTMKVEQ
     PVFQLEEVEF CQAHPVQFQG GWKMVRNVRT AMSKDVHCVN NIRDLATRRA WSNAQHHGGL
     ALSAGIPVVE TFYSRFKLYD VPRKHQRIDT VTNVHKWRGS GGSYVVTPES RASFWAAFGL
     TGDEQLALED RLERWEMDLF GEEGVDAHEP SILDSAVA