RDRP_TCV
ID RDRP_TCV Reviewed; 775 AA.
AC P17460; Q7TD17; Q7TD21; Q88486;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 3.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE AltName: Full=Protein p88;
DE Contains:
DE RecName: Full=Protein p28;
GN ORFNames=ORF1;
OS Turnip crinkle virus (TCV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC Tolivirales; Tombusviridae; Procedovirinae; Betacarmovirus.
OX NCBI_TaxID=11988;
OH NCBI_TaxID=3709; Brassica napus subsp. rapifera.
OH NCBI_TaxID=5130; Hypomyces.
OH NCBI_TaxID=180540; Moricandia arvensis (Purple mistress) (Brassica arvensis).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2718381; DOI=10.1016/0042-6822(89)90369-3;
RA Carrington J.C., Heaton L.A., Zuidema D., Hillman B.I., Morris T.J.;
RT "The genome structure of turnip crinkle virus.";
RL Virology 170:219-226(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Infectious clone UK;
RA Ryabov E.V.;
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP SEQUENCE REVISION.
RA Carrington J.C., Heaton L.A., Zuidema D., Hillman B.I., Morris T.J.;
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC the virus replication. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC 251.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M22445; AAA96968.2; -; Genomic_RNA.
DR EMBL; M22445; AAA96969.3; -; Genomic_RNA.
DR EMBL; AY312063; AAP78490.1; -; Genomic_RNA.
DR EMBL; AY312063; AAP78486.1; -; Genomic_RNA.
DR PIR; JA0110; RRVETC.
DR RefSeq; NP_620720.3; NC_003821.3.
DR RefSeq; NP_620721.2; NC_003821.3.
DR PRIDE; P17460; -.
DR GeneID; 944391; -.
DR GeneID; 944392; -.
DR KEGG; vg:944391; -.
DR KEGG; vg:944392; -.
DR Proteomes; UP000007403; Genome.
DR Proteomes; UP000009133; Genome.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 3.30.70.270; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR002166; RNA_pol_HCV.
DR Pfam; PF00998; RdRP_3; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW RNA suppression of termination; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication.
FT CHAIN 1..775
FT /note="RNA-directed RNA polymerase"
FT /id="PRO_0000040204"
FT CHAIN 1..250
FT /note="Protein p28"
FT /id="PRO_0000040205"
FT DOMAIN 473..586
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT VARIANT 42
FT /note="R -> Q (in strain: Infectious clone UK)"
FT VARIANT 56
FT /note="D -> S (in strain: Infectious clone UK)"
FT VARIANT 202
FT /note="D -> N (in strain: Infectious clone UK)"
FT VARIANT 345
FT /note="R -> K (in strain: Infectious clone UK)"
FT VARIANT 581
FT /note="D -> E (in strain: Infectious clone UK)"
FT VARIANT 717
FT /note="D -> N (in strain: Infectious clone UK)"
FT VARIANT 748
FT /note="Q -> E"
SQ SEQUENCE 775 AA; 87648 MW; A19CE0848C75253F CRC64;
MPLLHTLNTA LAVGLLGARY YPEVQTFLGL PDYVGHMKNV VRSVFQGSGL VVVSSDTVGV
RGTYSNRGQI GSSLGCILAV PDSGADIEID LDRLVGTEEE ATSCLVEAVG STADVPRRRV
RQKGRFAMHA VNAAKLHFCG VPKPTEANRL AVSKWLVQYC KERHVVDSHI RTIVNTALPR
VFTPDAEDIQ VVLDLHSVRA HDHRNALAEA GKVRKWWVNL AMHPMTGRSW SRAWRRLCRL
PDDQAISFVR XGCLRELVGR ETQISRGENP AMRVFPLANP PKVRRIFHIC GMGNGLDFGV
HNNSLNNLRR GLMERVFYVE DAQKQLKPAP QPIPGIFGKL SGIRRRLVRL AGNHTPVPRE
KYPSFYKGRR ATIYQKALDS LHDRPVSRKD AELKTFVKAE KINFTAKKDP APRVIQPRDP
RYNIEVGKYL KPYEHHLYRA IDAMWGGPTV LKGYDVGELG NIMSNTWDKF RKTCAIGFDM
KRFDQHVSVD ALRWEHSVYN AGFNCPELAQ LLTWQLTNKG VGRASDGFIK YQVDGCRMSG
DVNTALGNCL LACSITKYLM KGIKCKLINN GDDCVLFFEA DEVDRVRERL HHWIDFGFQC
IAEEPQYELE KVEFCQMSPI FDGEGWVMVR NPRVSLSKDS YSTTQWANEK DAARWLAAIG
ECGLAIAGGV PVLQSYYSCL KRNFGPLAGD YKKKMQDVSF DSGFYRLSKN GMRGSKDVSQ
DARFSFYRGF GYTPDEQEAL EEYYDNLQLL CEWDPTGYKE ELSDRWILNE FPTTL