ID   RDRP_TNVA               Reviewed;         724 AA.
AC   P22958;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   29-SEP-2021, entry version 87.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p82;
DE   Contains:
DE     RecName: Full=Protein p23;
GN   ORFNames=ORF1;
OS   Tobacco necrosis virus (strain A) (TNV-A).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Alphanecrovirus.
OX   NCBI_TaxID=12055;
OH   NCBI_TaxID=66262; Chenopodium amaranticolor.
OH   NCBI_TaxID=63459; Chenopodium quinoa (Quinoa).
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=81866; Nicotiana clevelandii (Wild tobacco).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=13306; Tulipa gesneriana (Garden tulip).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2371773; DOI=10.1016/0042-6822(90)90536-z;
RA   Meulewaeter F., Seurinck J., van Emmelo J.;
RT   "Genome structure of tobacco necrosis virus strain A.";
RL   Virology 177:699-709(1990).
CC   -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC       the virus replication. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC       203.
CC   -!- SIMILARITY: Belongs to the tombusviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; M33002; AAA86434.2; -; Genomic_RNA.
DR   PIR; A35523; RRWQTN.
DR   RefSeq; NP_056825.2; NC_001777.1.
DR   GeneID; 1493899; -.
DR   KEGG; vg:1493899; -.
DR   Proteomes; UP000000575; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   InterPro; IPR013707; Tombusvirus_p33.
DR   Pfam; PF00998; RdRP_3; 1.
DR   Pfam; PF08500; Tombus_P33; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA suppression of termination; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..724
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000040217"
FT   CHAIN           1..202
FT                   /note="Protein p23"
FT                   /id="PRO_0000040218"
FT   DOMAIN          424..540
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   VARIANT         72
FT                   /note="V -> A"
FT   VARIANT         699
FT                   /note="K -> R"
SQ   SEQUENCE   724 AA;  82279 MW;  87E6761AA1BA2164 CRC64;
     MELPNQHKQT AAEGFVSFLN WLCNPWRRQR TVNAAVAFQK DLLAIEDSEH LDDINECFEE
     SAGAQSQRTK VVADGAYAPA KSNRTRRVRK QKKHKFVKYL VNEARAEFGL PKPTEANRLM
     VQHFLLRVCK DWGVVTAHVH GNVALALPLV FIPTEDDLLS RALMNTHATR AAVRGMDNVQ
     GEGWWNNRLG IGGQVGLAFR SKXGCLERRP GFSTSVSRGE HPDLVVIPSG RPEKQRQLLR
     YSGIGGHLLI GIHNNSLSNL RRGLMERVFY VEGPNGLQDA PKPVKGAFRT LDKFRDLYTK
     NSWRHTPVTS EQFLMNYTGR KLTIYREAVD SLSHQPLSSR DAKLKTFVKA EKLNLSKKPD
     PAPRVIQPRS PRYNVCLGRY LRHYEHHAFK TIAKCFGEIT VFKGFTLEQQ GEIMRSKWNK
     YVNPVAVGLD ASRFDQHVSV EALEYEHEFY LRDYPNDKQL KWLLKQQLCN VGTAFASDGI
     IKYKKKGCRM SGDMNTSLGN CILMCAMVYG LKEHLNINLS LANNGDDCVI VCEKADLKKL
     TSSIEPYFKQ FGFKMEVEKP VDIFERIEFC QTQPVFDGSQ YIMVRKPSVV TSKDVTSLIP
     CQTKAQYAEW LQAVGECGMS INGGIPVMQN FYQKLQTGIR RTKFTKTGEF QTNGLGYHSR
     YMHRVARVPS PETRLSFYLA FGITPDLQEA LEIFYDTHKL ELDDVIPTDT YQVSGEHLIN
     GLPN