ID   RDRP_TNVD               Reviewed;         725 AA.
AC   P27209;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 3.
DT   29-SEP-2021, entry version 77.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p82;
DE   Contains:
DE     RecName: Full=Protein p23;
GN   ORFNames=ORF1;
OS   Tobacco necrosis virus (strain D) (TNV-D).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Betanecrovirus.
OX   NCBI_TaxID=12056;
OH   NCBI_TaxID=66262; Chenopodium amaranticolor.
OH   NCBI_TaxID=63459; Chenopodium quinoa (Quinoa).
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=81866; Nicotiana clevelandii (Wild tobacco).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=13306; Tulipa gesneriana (Garden tulip).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1856691; DOI=10.1099/0022-1317-72-7-1521;
RA   Coutts R.H.A., Rigden J.E., Slabas A.R., Lomonossoff G.P., Wise P.J.;
RT   "The complete nucleotide sequence of tobacco necrosis virus strain D.";
RL   J. Gen. Virol. 72:1521-1529(1991).
RN   [2]
RP   SEQUENCE REVISION.
RA   Coutts R.H.A.;
RL   Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC       the virus replication. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC       203.
CC   -!- SIMILARITY: Belongs to the tombusviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; D00942; BAA00785.1; -; Genomic_RNA.
DR   EMBL; D00942; BAA00786.1; -; Genomic_RNA.
DR   PIR; JU0368; RRWQTD.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   InterPro; IPR013707; Tombusvirus_p33.
DR   Pfam; PF00998; RdRP_3; 1.
DR   Pfam; PF08500; Tombus_P33; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA suppression of termination;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..725
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000040219"
FT   CHAIN           1..202
FT                   /note="Protein p23"
FT                   /id="PRO_0000040220"
FT   DOMAIN          424..544
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   725 AA;  82357 MW;  5A57467158A30634 CRC64;
     MESLPIVLLS LISKAVVLIC SLLTLIIQNS TAVTWGLACV WLAYVSFRFL FQVKITVHPA
     ARETFESMVR KFQAESMFSE EATPCIVSVG DKDADLTPDP QREDIKIVKS SRRVSYAVRV
     AHVAKAQVGL LANSRANELV YSRLCREEMV KHGVRPSHIA HMVPLAVAAC FIPLDSDFLA
     ASIRQGEGMR ERRALLGPSW EKXGGLLVTS GFTTPTWRGD PRGMLVTKGP PLAKPRKLYR
     FTGMGTHIRY GVHDHSLGNV RRGLVERLYM VEVKGELQPT PKPTPGAFNQ MSRFSDRLSI
     HLPKTTRLTP REFLGFYTGR KLERYQKAVE SLEMHPVREK DAWLSTFVKA EKLNITAKPD
     PAPRVIQPRD PRYNVEVGRF LRHSEEMLFK AINKTFGGRT IFKGLSSDQA GEEFKTLWDS
     FKDPVGIGMD ASRFDQHISK DALEFEHKMW LSMFPKSERA ELARLLSWQI NNRGLARCPD
     GEIRYRVEGC RMSGDMNTSS GNCYIMCATV HNWCDNIKHI KHFRLANNGD DCMLVVERSD
     EKKVRNGLIE YYATLGFTMK VEPTVDVLER LEFCQTRPVL VDGKYRMVRN LHQSMSKDLH
     SLHDLDSSAA RNAWVTAVGT GGRCMNDGVP VLKEFFKQFP DYNLEVKKGS DMAQKLRDDW
     KYKFNRTAAF QDLIPTQESR YSFWLAFGLL PDEQIALENG FSPLKMEIVN EQIQEETSLL
     QFSGA