ID   RDRP_TOBSV              Reviewed;         800 AA.
AC   P89678;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   29-SEP-2021, entry version 70.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=2a pol;
DE            EC=2.7.7.48;
DE   AltName: Full=2a polymerase;
GN   ORFNames=ORF2a;
OS   Tobacco streak virus (strain WC) (TSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Ilarvirus.
OX   NCBI_TaxID=12318;
OH   NCBI_TaxID=4686; Asparagus officinalis (Garden asparagus).
OH   NCBI_TaxID=41562; Dahlia.
OH   NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH   NCBI_TaxID=34274; Gossypium herbaceum (Levant cotton) (Arabian cotton).
OH   NCBI_TaxID=47082; Melilotus albus (White sweet clover) (Melilotus officinalis subsp. albus).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=137000; Rosa setigera.
OH   NCBI_TaxID=57577; Trifolium pratense (Red clover).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9687875; DOI=10.1007/s007050050366;
RA   Scott S.W., Zimmerman M.T., Ge X.;
RT   "The sequence of RNA 1 and RNA 2 of tobacco streak virus: additional
RT   evidence for the inclusion of alfalfa mosaic virus in the genus
RT   Ilarvirus.";
RL   Arch. Virol. 143:1187-1198(1998).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; U75538; AAB48409.1; -; Genomic_RNA.
DR   RefSeq; NP_620768.1; NC_003842.1.
DR   GeneID; 962656; -.
DR   KEGG; vg:962656; -.
DR   Proteomes; UP000007795; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..800
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000402393"
FT   DOMAIN          459..574
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          766..800
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        772..789
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   800 AA;  91545 MW;  5D19CDDDCD1DC5A5 CRC64;
     MDLVIKNLIV YHLRRRIDIG TSFGIEPADY IDWVKVFLLK FIIEHTARFA DFATIHTTML
     LVLGEDDPNY VEKDTPIMEI DPFYLPYDDL DVDYTSLRVC GDEDQSCSDR DELSDFISNI
     SHIPEGTSWG SESDTSFVEH LETIQDIPTK CEIADKPVEE IPFDDDGKVV NDVWVDAELS
     NAPEISCDAD IRACGFVSLR LLESSRGYPK WTPERVSSGL NPDLPVNSKP AVDEIFPHHH
     SVDDRFFQEW VETHDIDLEV TSCDLDMSTF NDWTKGVDTR LVPNMSVGGL SHRVPTQREA
     LLAIKKRNMN VPELQSNFDH DDVLNRCVTR FITHVVDKTR LSKLNPISGE ELHYFNQYLE
     NKNPPLSEYK GPVPLVALDK YMHMIKTTLK PVEEDNLHIE RPIPATITYH KKGVVMMTSP
     YFLCAMVRLL YVLKSKFVVP TGKYHQIFQM NPELLKHSKE FKEIDFSKFD KSQGRLHHDV
     QFRLFLALGI PEHFVTTWFN SHEKSYIRDR DCGIGFSVDY QRRTGDACTY LGNTLVTLSV
     LSYVYDLSNP NILFVAASGD DSLIGSIEPL PREKEDLCVS LFNFETKFPH NQPFICSKFL
     LVVECDDGSE EVLAVPNPLK LLQKLGPKNL QVTVLDDYYQ SLCDILWVFN DADVCRRTAE
     LAEYRRFKGT KKCLFLESAL LSLPSLVANR MKFVRRTINL ESSRACIRND VYSDLVPHFD
     SRVSRCDDSD GVRTSTFDDR KSSKHASDKL RKTECYGEAR CRIKPRRNRK SESGAVQYSQ
     SSGIETGKAN SSRKGRIKLH