ID   RDRP_TRVPS              Reviewed;         179 AA.
AC   P05079;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   23-FEB-2022, entry version 63.
DE   RecName: Full=Replicase large subunit;
DE            EC=2.1.1.-;
DE            EC=2.7.7.48;
DE            EC=3.6.4.13;
DE   Flags: Fragment;
OS   Tobacco rattle virus (strain PSG).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Virgaviridae; Tobravirus.
OX   NCBI_TaxID=12297;
OH   NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH   NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH   NCBI_TaxID=82024; Hyacinthus.
OH   NCBI_TaxID=39639; Narcissus pseudonarcissus (Daffodil).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH   NCBI_TaxID=13274; Stellaria media (Common chickweed) (Alsine media).
OH   NCBI_TaxID=13305; Tulipa.
OH   NCBI_TaxID=97415; Viola arvensis (European field pansy) (Field violet).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3960718; DOI=10.1093/nar/14.5.2157;
RA   Cornelissen B.J.C., Linthorst H.J.M., Brederode F.T., Bol J.F.;
RT   "Analysis of the genome structure of tobacco rattle virus strain PSG.";
RL   Nucleic Acids Res. 14:2157-2169(1986).
CC   -!- FUNCTION: Replicase large subunit: is an RNA-dependent RNA polymerase
CC       active in viral RNA replication. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; X03685; CAA27319.1; -; Genomic_RNA.
DR   PIR; A05248; A05248.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA suppression of termination;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           <1..179
FT                   /note="Replicase large subunit"
FT                   /id="PRO_0000222513"
FT   NON_TER         1
SQ   SEQUENCE   179 AA;  19950 MW;  A321512687CA92D4 CRC64;
     GAHLVPTKSG DADTYNANSD RTLCALLSEL PLEKAVMVTY GGDDSLIAFP RGTQFVDPCP
     KLATKWNFEC KIFKYDVPMF CGKFLLKTSS CYEFVPDPVK VLTKLGKKSI KDVQHLAEIY
     ISLNDSNRAL GNYMVVSKLS ESVSDRYLYK GDSVHALCAL WKHIKSFTAL CTLLPRRKG