RDS3_YEAST
ID RDS3_YEAST Reviewed; 107 AA.
AC Q06835; D6W493;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Pre-mRNA-splicing factor RDS3;
DE AltName: Full=Regulator of drug sensitivity 3;
GN Name=RDS3; OrderedLocusNames=YPR094W; ORFNames=P9513.2;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION.
RX PubMed=11943786; DOI=10.1074/jbc.m202566200;
RA Akache B., Turcotte B.;
RT "New regulators of drug sensitivity in the family of yeast zinc cluster
RT proteins.";
RL J. Biol. Chem. 277:21254-21260(2002).
RN [5]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=14517302; DOI=10.1128/mcb.23.20.7339-7349.2003;
RA Wang Q., Rymond B.C.;
RT "Rds3p is required for stable U2 snRNP recruitment to the splicing
RT apparatus.";
RL Mol. Cell. Biol. 23:7339-7349(2003).
CC -!- FUNCTION: Required for pre-mRNA splicing. Involved in regulation of
CC drug sensitivity and may play a role in multidrug resistance.
CC {ECO:0000269|PubMed:11943786, ECO:0000269|PubMed:14517302}.
CC -!- SUBUNIT: Component of the spliceosome where it interacts with CUS1,
CC HSH49, HSH155, IST3 and RSE1. Also interacts with YRA1.
CC {ECO:0000269|PubMed:14517302}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14517302}.
CC -!- SIMILARITY: Belongs to the PHF5 family. {ECO:0000305}.
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DR EMBL; U51033; AAB68140.1; -; Genomic_DNA.
DR EMBL; AY693180; AAT93199.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11509.1; -; Genomic_DNA.
DR PIR; S69077; S69077.
DR RefSeq; NP_015419.1; NM_001184191.1.
DR PDB; 2K0A; NMR; -; A=2-107.
DR PDB; 5GM6; EM; 3.50 A; J=1-107.
DR PDB; 5LQW; EM; 5.80 A; Y=1-107.
DR PDB; 5NRL; EM; 7.20 A; S=1-107.
DR PDB; 5ZWM; EM; 3.40 A; 5=1-107.
DR PDB; 5ZWO; EM; 3.90 A; 5=1-107.
DR PDB; 6G90; EM; 4.00 A; S=1-107.
DR PDB; 7OQB; EM; 9.00 A; S=1-107.
DR PDB; 7OQE; EM; 5.90 A; S=1-107.
DR PDBsum; 2K0A; -.
DR PDBsum; 5GM6; -.
DR PDBsum; 5LQW; -.
DR PDBsum; 5NRL; -.
DR PDBsum; 5ZWM; -.
DR PDBsum; 5ZWO; -.
DR PDBsum; 6G90; -.
DR PDBsum; 7OQB; -.
DR PDBsum; 7OQE; -.
DR AlphaFoldDB; Q06835; -.
DR BMRB; Q06835; -.
DR SMR; Q06835; -.
DR BioGRID; 36262; 227.
DR ComplexPortal; CPX-1647; SF3B complex.
DR ComplexPortal; CPX-26; U2 small nuclear ribonucleoprotein complex.
DR DIP; DIP-3822N; -.
DR IntAct; Q06835; 10.
DR STRING; 4932.YPR094W; -.
DR MaxQB; Q06835; -.
DR PaxDb; Q06835; -.
DR PRIDE; Q06835; -.
DR EnsemblFungi; YPR094W_mRNA; YPR094W; YPR094W.
DR GeneID; 856209; -.
DR KEGG; sce:YPR094W; -.
DR SGD; S000006298; RDS3.
DR VEuPathDB; FungiDB:YPR094W; -.
DR eggNOG; KOG1705; Eukaryota.
DR GeneTree; ENSGT00390000018518; -.
DR HOGENOM; CLU_110369_1_1_1; -.
DR InParanoid; Q06835; -.
DR OMA; AYYCWEC; -.
DR BioCyc; YEAST:G3O-34235-MON; -.
DR EvolutionaryTrace; Q06835; -.
DR PRO; PR:Q06835; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q06835; protein.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0005686; C:U2 snRNP; IDA:SGD.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IPI:ComplexPortal.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:SGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IMP:SGD.
DR GO; GO:0000245; P:spliceosomal complex assembly; IDA:SGD.
DR GO; GO:1903241; P:U2-type prespliceosome assembly; IC:ComplexPortal.
DR InterPro; IPR005345; PHF5.
DR PANTHER; PTHR13120; PTHR13120; 1.
DR Pfam; PF03660; PHF5; 1.
DR PIRSF; PIRSF016468; PHF5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Spliceosome.
FT CHAIN 1..107
FT /note="Pre-mRNA-splicing factor RDS3"
FT /id="PRO_0000218722"
FT STRAND 15..19
FT /evidence="ECO:0007829|PDB:2K0A"
FT HELIX 24..26
FT /evidence="ECO:0007829|PDB:2K0A"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:2K0A"
FT STRAND 44..46
FT /evidence="ECO:0007829|PDB:2K0A"
FT HELIX 47..50
FT /evidence="ECO:0007829|PDB:2K0A"
FT TURN 59..61
FT /evidence="ECO:0007829|PDB:2K0A"
FT STRAND 62..65
FT /evidence="ECO:0007829|PDB:2K0A"
FT HELIX 74..79
FT /evidence="ECO:0007829|PDB:2K0A"
SQ SEQUENCE 107 AA; 12260 MW; F0E4737CA4FE8D24 CRC64;
MSRHQFDLIM CLKQPGVQTG LLCEKCDGKC PICDSYVRPK RKVRVCENCS FGKQAKNCII
CNLNVGVNDA FYCWECCRLG KDKDGCPRIL NLGSNRLDRH FEKKKKV
