RE107_YEAST
ID RE107_YEAST Reviewed; 314 AA.
AC P21651; D6VWJ5;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Recombination protein 107;
DE AltName: Full=Meiotic recombination protein 2;
GN Name=REC107; Synonyms=MER2; OrderedLocusNames=YJR021C; ORFNames=J1462;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2203538; DOI=10.1016/0092-8674(90)90267-i;
RA Engebrecht J., Hirsch J., Roeder G.S.;
RT "Meiotic gene conversion and crossing over: their relationship to each
RT other and to chromosome synapsis and segregation.";
RL Cell 62:927-937(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1840507; DOI=10.1016/0092-8674(91)90047-3;
RA Engebrecht J., Voelkel-Meiman K., Roeder G.S.;
RT "Meiosis-specific RNA splicing in yeast.";
RL Cell 66:1257-1268(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 213-314.
RC STRAIN=DH484;
RX PubMed=7781779; DOI=10.1016/0014-5793(95)00471-k;
RA Hani J., Stumpf G., Domdey H.;
RT "PTF1 encodes an essential protein in Saccharomyces cerevisiae, which shows
RT strong homology with a new putative family of PPIases.";
RL FEBS Lett. 365:198-202(1995).
RN [6]
RP IDENTIFICATION OF REC107 AS MER2.
RX PubMed=1545806; DOI=10.1128/mcb.12.3.1248-1256.1992;
RA Cool M., Malone R.E.;
RT "Molecular and genetic analysis of the yeast early meiotic recombination
RT genes REC102 and REC107/MER2.";
RL Mol. Cell. Biol. 12:1248-1256(1992).
RN [7]
RP INTERACTION WITH MEI4; REC114 AND XRS2.
RX PubMed=14992724; DOI=10.1016/s1097-2765(04)00063-2;
RA Arora C., Kee K., Maleki S., Keeney S.;
RT "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break
RT formation, independent of its cytoplasmic role in RNA metabolism.";
RL Mol. Cell 13:549-559(2004).
RN [8]
RP IDENTIFICATION IN THE MER2-MEI4-REC114 COMPLEX, FUNCTION OF THE
RP MER2-MEI4-REC114 COMPLEX, INTERACTION WITH MEI4 AND REC114, SUBCELLULAR
RP LOCATION, AND PHOSPHORYLATION.
RX PubMed=16783010; DOI=10.1534/genetics.106.058768;
RA Li J., Hooker G.W., Roeder G.S.;
RT "Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for
RT meiotic double-strand break formation.";
RL Genetics 173:1969-1981(2006).
CC -!- FUNCTION: Essential for meiotic chromosome segregation. MER1 and MER2
CC proteins must interact directly or indirectly. MER1 might be
CC responsible for regulating the MER2 gene and/or gene product. MER2 is
CC not required for mitosis and mitotic DNA repair mechanisms. Component
CC of the MER2-MEI4-REC114 complex which seems to be required for meiotic
CC double-strand break (DSB) formation. {ECO:0000269|PubMed:16783010}.
CC -!- SUBUNIT: Interacts with MEI4, REC114 and XRS2. Component of the MER2-
CC MEI4-REC114 complex. {ECO:0000269|PubMed:14992724,
CC ECO:0000269|PubMed:16783010}.
CC -!- INTERACTION:
CC P21651; P29467: MEI4; NbExp=7; IntAct=EBI-10742, EBI-2566907;
CC P21651; P21651: REC107; NbExp=8; IntAct=EBI-10742, EBI-10742;
CC P21651; P32841: REC114; NbExp=5; IntAct=EBI-10742, EBI-14447;
CC P21651; Q03012: SPP1; NbExp=3; IntAct=EBI-10742, EBI-32540;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16783010}. Chromosome
CC {ECO:0000269|PubMed:16783010}. Note=Localizes to chromosomes.
CC -!- PTM: Phosphorylated during meiotic prophase. Dephosphorylation seems to
CC be dependent on DSB formation. {ECO:0000269|PubMed:16783010}.
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DR EMBL; M38340; AAA34772.1; -; Genomic_DNA.
DR EMBL; M60755; AAA34773.1; -; mRNA.
DR EMBL; X87611; CAA60944.1; -; Genomic_DNA.
DR EMBL; Z49521; CAA89546.1; -; Genomic_DNA.
DR EMBL; Z49519; CAA89545.1; -; Genomic_DNA.
DR EMBL; X85972; CAA59959.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08811.1; -; Genomic_DNA.
DR PIR; S55210; S55210.
DR RefSeq; NP_012555.1; NM_001181679.1.
DR AlphaFoldDB; P21651; -.
DR SMR; P21651; -.
DR BioGRID; 33775; 117.
DR ComplexPortal; CPX-1809; MER2-MEI4-REC114 meiotic recombination initiation complex.
DR DIP; DIP-5265N; -.
DR IntAct; P21651; 9.
DR STRING; 4932.YJR021C; -.
DR CarbonylDB; P21651; -.
DR iPTMnet; P21651; -.
DR PaxDb; P21651; -.
DR PRIDE; P21651; -.
DR EnsemblFungi; YJR021C_mRNA; YJR021C; YJR021C.
DR GeneID; 853478; -.
DR KEGG; sce:YJR021C; -.
DR SGD; S000003782; REC107.
DR VEuPathDB; FungiDB:YJR021C; -.
DR eggNOG; ENOG502S5X2; Eukaryota.
DR HOGENOM; CLU_1046568_0_0_1; -.
DR InParanoid; P21651; -.
DR OMA; KWAGKLE; -.
DR BioCyc; YEAST:G3O-31661-MON; -.
DR PRO; PR:P21651; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P21651; protein.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IC:ComplexPortal.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR InterPro; IPR015159; Rec107.
DR Pfam; PF09074; Mer2; 1.
PE 1: Evidence at protein level;
KW Chromosome; DNA recombination; Meiosis; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..314
FT /note="Recombination protein 107"
FT /id="PRO_0000096421"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..282
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..314
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 279
FT /note="N -> I (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 284..314
FT /note="DNDDASHRLKRAARTIIPWEELRPDTLESEL -> TMMTLAIG (in Ref.
FT 1 and 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 314 AA; 35683 MW; 46F426AC0DFDFAD0 CRC64;
MVARGRTDEI STDVSEANSE HSLMITETSS PFRSIFSHSG KVANAGALEE SDKQILEWAG
KLELESMELR ENSDKLIKVL NENSKTLCKS LNKFNQLLEQ DAATNGNVKT LIKDLASQIE
NQLDKVSTAM LSKGDEKKTK SDSSYRQVLV EEISRYNSKI TRHVTNKQHE TEKSMRCTQE
MLFNVGSQLE DVHKVLLSLS KDMHSLQTRQ TALEMAFREK ADHAYDRPDV SLNGTTLLHD
MDEAHDKQRK KSVPPPRMMV TRSMKRRRSS SPTLSTSQNH NSEDNDDASH RLKRAARTII
PWEELRPDTL ESEL
