RE114_YEAST
ID RE114_YEAST Reviewed; 428 AA.
AC P32841; D6VZV6;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 3.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Meiotic recombination protein REC114;
GN Name=REC114; OrderedLocusNames=YMR133W; ORFNames=YM9375.02;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8385581; DOI=10.1007/bf00310890;
RA Pittman D.L., Lu W., Malone R.E.;
RT "Genetic and molecular analysis of REC114, an early meiotic recombination
RT gene in yeast.";
RL Curr. Genet. 23:295-304(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP REVISION OF GENE MODEL.
RX PubMed=9267437; DOI=10.1007/s004380050513;
RA Malone R.E., Pittman D.L., Nau J.J.;
RT "Examination of the intron in the meiosis-specific recombination gene
RT REC114 in Saccharomyces.";
RL Mol. Gen. Genet. 255:410-419(1997).
RN [5]
RP FUNCTION.
RX PubMed=9177999; DOI=10.1093/genetics/146.2.481;
RA Galbraith A.M., Bullard S.A., Jiao K., Nau J.J., Malone R.E.;
RT "Recombination and the progression of meiosis in Saccharomyces
RT cerevisiae.";
RL Genetics 146:481-489(1997).
RN [6]
RP FUNCTION.
RX PubMed=10526232; DOI=10.1046/j.1365-2443.1999.00273.x;
RA Bishop D.K., Nikolski Y., Oshiro J., Chon J., Shinohara M., Chen X.;
RT "High copy number suppression of the meiotic arrest caused by a dmc1
RT mutation: REC114 imposes an early recombination block and RAD54 promotes a
RT DMC1-independent DSB repair pathway.";
RL Genes Cells 4:425-444(1999).
RN [7]
RP INTERACTION WITH MEI4; MER2 AND REC104.
RX PubMed=14992724; DOI=10.1016/s1097-2765(04)00063-2;
RA Arora C., Kee K., Maleki S., Keeney S.;
RT "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break
RT formation, independent of its cytoplasmic role in RNA metabolism.";
RL Mol. Cell 13:549-559(2004).
RN [8]
RP FUNCTION.
RX PubMed=15655113; DOI=10.1101/gad.321105;
RA Prieler S., Penkner A., Borde V., Klein F.;
RT "The control of Spo11's interaction with meiotic recombination hotspots.";
RL Genes Dev. 19:255-269(2005).
RN [9]
RP IDENTIFICATION IN THE MER2-MEI4-REC114 COMPLEX, FUNCTION OF THE
RP MER2-MEI4-REC114 COMPLEX, INTERACTION WITH MEI4 AND MER2, AND SUBCELLULAR
RP LOCATION.
RX PubMed=16783010; DOI=10.1534/genetics.106.058768;
RA Li J., Hooker G.W., Roeder G.S.;
RT "Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for
RT meiotic double-strand break formation.";
RL Genetics 173:1969-1981(2006).
CC -!- FUNCTION: Required for the production of double-strand breaks (DSBs) in
CC meiotic recombination initiation; not required for mitosis. Plays a
CC role in activation of the DSB nuclease SPO11 at recombination hotspots.
CC Prevents accumulation of meiotic double strand break intermediates and
CC is needed for the proper timing of the first meiotic division.
CC Component of the MER2-MEI4-REC114 complex which seems to be required
CC for meiotic double-strand break (DSB) formation.
CC {ECO:0000269|PubMed:10526232, ECO:0000269|PubMed:15655113,
CC ECO:0000269|PubMed:16783010, ECO:0000269|PubMed:9177999}.
CC -!- SUBUNIT: Interacts with MEI4, MER2 and REC104. Component of the MER2-
CC MEI4-REC114 complex. {ECO:0000269|PubMed:14992724,
CC ECO:0000269|PubMed:16783010}.
CC -!- INTERACTION:
CC P32841; P29467: MEI4; NbExp=8; IntAct=EBI-14447, EBI-2566907;
CC P32841; P21651: REC107; NbExp=5; IntAct=EBI-14447, EBI-10742;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16783010}. Chromosome
CC {ECO:0000269|PubMed:16783010}. Note=Localizes to chromosomes.
CC -!- DEVELOPMENTAL STAGE: Meiosis-specific.
CC -!- SIMILARITY: Belongs to the REC114 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA87347.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z14315; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z47071; CAA87347.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006946; DAA10030.1; -; Genomic_DNA.
DR PIR; S27453; S27453.
DR RefSeq; NP_013852.1; NM_001182634.1.
DR AlphaFoldDB; P32841; -.
DR SMR; P32841; -.
DR BioGRID; 35310; 66.
DR ComplexPortal; CPX-1809; MER2-MEI4-REC114 meiotic recombination initiation complex.
DR DIP; DIP-4006N; -.
DR IntAct; P32841; 5.
DR STRING; 4932.YMR133W; -.
DR PaxDb; P32841; -.
DR PRIDE; P32841; -.
DR EnsemblFungi; YMR133W_mRNA; YMR133W; YMR133W.
DR GeneID; 855163; -.
DR KEGG; sce:YMR133W; -.
DR SGD; S000004740; REC114.
DR VEuPathDB; FungiDB:YMR133W; -.
DR eggNOG; ENOG502S7Q1; Eukaryota.
DR HOGENOM; CLU_704167_0_0_1; -.
DR InParanoid; P32841; -.
DR OMA; KSGNTHT; -.
DR BioCyc; YEAST:G3O-32826-MON; -.
DR PRO; PR:P32841; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P32841; protein.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; TAS:SGD.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR InterPro; IPR004354; Meiotic_Rec114.
DR Pfam; PF03525; Meiotic_rec114; 1.
DR PRINTS; PR01548; MEIOTICR114.
PE 1: Evidence at protein level;
KW Chromosome; DNA recombination; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..428
FT /note="Meiotic recombination protein REC114"
FT /id="PRO_0000097219"
FT REGION 255..278
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 307..380
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..331
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 346..380
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 96..97
FT /note="KY -> N (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 100..103
FT /note="EEFG -> RRIR (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 377
FT /note="S -> C (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 428 AA; 48706 MW; 57CB835DA71FBA9B CRC64;
MYEYCSVVIK KYSKYTIPSF APNGFQSMLE PPQIDKWQHL SANCTLQFRV LLMDSRQILI
NVVLNNSTLL ENIRLPLGDN QDLIQFSCKS PIISCKYISE EFGPRMLRRF QMNLPNDVEF
NRTVVSLKNL NFVLRTARTS IAQSTITSQV QGNNNGTKVC FTEGPKVSSY TNPNTQFQTQ
NMIMDFSQRY QEESERESNN RSNITLPHDS IQIAQQIWPN TDLNVVQSSQ DLNTPMATQT
VLGRPESLIV QPLEVSQSPP NTTNCLPNAE NKKKKVDTTS DFTSRKEIAL CKTGLLETIH
IPKERESQMQ SVTGLDATPT IIWSPGKDNT AKKNTSNKKN IDDKLTNPQK SGNTHTPDRN
KEVLPNGTLN ETRKEASPSE GLTIRVKNVN RNASRKISKR LIKEKLKDEE FMKWVNKVET
VLNKMFEK
