ID   REBF_LENAE              Reviewed;         170 AA.
AC   Q8KI76;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Flavin reductase (NADPH);
DE            EC=1.5.1.30;
DE   AltName: Full=Flavin dependent oxidoreductase;
DE   AltName: Full=Flavin:NAD(P)H reductase;
GN   Name=rbmH; Synonyms=rebF;
OS   Lentzea aerocolonigenes (Lechevalieria aerocolonigenes) (Saccharothrix
OS   aerocolonigenes).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Lentzea.
OX   NCBI_TaxID=68170;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 39243 / DSM 44217 / BCRC 13729 / KCTC 9384;
RX   PubMed=11983340; DOI=10.1016/s1074-5521(02)00126-6;
RA   Sanchez C., Butovich I.A., Brana A.F., Rohr J., Mendez C., Salas J.A.;
RT   "The biosynthetic gene cluster for the antitumor rebeccamycin:
RT   characterization and generation of indolocarbazole derivatives.";
RL   Chem. Biol. 9:519-531(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12617516; DOI=10.7164/antibiotics.55.1063;
RA   Onaka H., Taniguchi S., Igarashi Y., Furumai T.;
RT   "Cloning of the staurosporine biosynthetic gene cluster from Streptomyces
RT   sp. TP-A0274 and its heterologous expression in Streptomyces lividans.";
RL   J. Antibiot. 55:1063-1071(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Hyun C.-G., Bililign T., Liao J., Thorson J.S.;
RT   "The Biosynthesis of Indolocarbazoles in a Heterologous E. coli Host.";
RL   Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12619684; DOI=10.1271/bbb.67.127;
RA   Onaka H., Taniguchi S., Igarashi Y., Furumai T.;
RT   "Characterization of the biosynthetic gene cluster of rebeccamycin from
RT   Lechevalieria aerocolonigenes ATCC 39243.";
RL   Biosci. Biotechnol. Biochem. 67:127-138(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=14763561; DOI=10.7164/antibiotics.56.950;
RA   Onaka H., Taniguchi S., Ikeda H., Igarashi Y., Furumai T.;
RT   "pTOYAMAcos, pTYM18, and pTYM19, actinomycete-Escherichia coli integrating
RT   vectors for heterologous gene expression.";
RL   J. Antibiot. 56:950-956(2003).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15743957; DOI=10.1128/jb.187.6.2084-2092.2005;
RA   Nishizawa T., Aldrich C.C., Sherman D.H.;
RT   "Molecular analysis of the rebeccamycin L-amino acid oxidase from
RT   Lechevalieria aerocolonigenes ATCC 39243.";
RL   J. Bacteriol. 187:2084-2092(2005).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Onaka H.;
RT   "Biosynthesis of heterocyclic antibiotics in actinomycetes and an approach
RT   to synthesize the natural compounds.";
RL   Nihon Hosenkin Gakkaishi 20:62-71(2006).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Asamizu S., Kato Y., Igarashi Y., Furumai T., Onaka H.;
RT   "Direct formation of chromopyrrolic acid from indole-3-pyruvic acid by
RT   StaD, a novel hemoprotein in indolocarbazole biosynthesis.";
RL   Tetrahedron Lett. 47:473-475(2006).
RN   [9]
RP   FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=15743914; DOI=10.1073/pnas.0500755102;
RA   Yeh E., Garneau S., Walsh C.T.;
RT   "Robust in vitro activity of RebF and RebH, a two-component
RT   reductase/halogenase, generating 7-chlorotryptophan during rebeccamycin
RT   biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3960-3965(2005).
CC   -!- FUNCTION: Catalyzes the NADH-dependent reduction of FAD to provide
CC       FADH2 for the halogenase RebH. {ECO:0000269|PubMed:11983340,
CC       ECO:0000269|PubMed:15743914}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + reduced riboflavin = 2 H(+) + NADPH + riboflavin;
CC         Xref=Rhea:RHEA:19377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17607,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:57986, ChEBI:CHEBI:58349; EC=1.5.1.30;
CC         Evidence={ECO:0000269|PubMed:15743914};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.7 uM for NADH (with FAD) {ECO:0000269|PubMed:15743914};
CC         KM=1.3 uM for NADH (with FMN) {ECO:0000269|PubMed:15743914};
CC         Note=kcat is 108 min(-1).;
CC   -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC       {ECO:0000305}.
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DR   EMBL; AF534707; AAN01214.1; -; Genomic_DNA.
DR   EMBL; AB090952; BAC10680.1; -; Genomic_DNA.
DR   EMBL; AB071405; BAC15756.1; -; Genomic_DNA.
DR   EMBL; AJ414559; CAC93720.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8KI76; -.
DR   SMR; Q8KI76; -.
DR   BioCyc; MetaCyc:MON-15091; -.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0042602; F:riboflavin reductase (NADPH) activity; IDA:CACAO.
DR   Gene3D; 2.30.110.10; -; 1.
DR   InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR   InterPro; IPR012349; Split_barrel_FMN-bd.
DR   Pfam; PF01613; Flavin_Reduct; 1.
DR   SMART; SM00903; Flavin_Reduct; 1.
PE   1: Evidence at protein level;
KW   NADP; Oxidoreductase.
FT   CHAIN           1..170
FT                   /note="Flavin reductase (NADPH)"
FT                   /id="PRO_0000422335"
SQ   SEQUENCE   170 AA;  18435 MW;  1F34D19650D1D677 CRC64;
     MTIEFDRPGA HVTAADHRAL MSLFPTGVAV ITAIDEAGTP HGMTCTSLTS VTLDPPTLLV
     CLNRASGTLH AVRGGRFGVN LLHARGRRAA EVFSTAVQDR FGEVRWEHSD VTGMPWLAED
     AHAFAGCVVR KSTVVGDHEI VLGEVHEVVR EHDLPLLYGM REFAVWTPEG