REBG_LENAE
ID REBG_LENAE Reviewed; 421 AA.
AC Q8KHE4; Q9S595;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=4'-demethylrebeccamycin synthase;
DE EC=4.3.3.5;
DE AltName: Full=Arcyriaflavin A N-glycosyltransferase;
GN Name=rebG; Synonyms=rbmA;
OS Lentzea aerocolonigenes (Lechevalieria aerocolonigenes) (Saccharothrix
OS aerocolonigenes).
OC Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Lentzea.
OX NCBI_TaxID=68170;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 39243 / DSM 44217 / BCRC 13729 / KCTC 9384;
RX PubMed=10866221; DOI=10.7164/antibiotics.53.393;
RA Ohuchi T., Ikeda-Araki A., Watanabe-Sakamoto A., Kojiri K., Nagashima M.,
RA Okanishi M., Suda H.;
RT "Cloning and expression of a gene encoding N-glycosyltransferase (ngt) from
RT Saccarothrix aerocolonigenes ATCC39243.";
RL J. Antibiot. 53:393-403(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 39243 / DSM 44217 / BCRC 13729 / KCTC 9384;
RX PubMed=11983340; DOI=10.1016/s1074-5521(02)00126-6;
RA Sanchez C., Butovich I.A., Brana A.F., Rohr J., Mendez C., Salas J.A.;
RT "The biosynthetic gene cluster for the antitumor rebeccamycin:
RT characterization and generation of indolocarbazole derivatives.";
RL Chem. Biol. 9:519-531(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 39243 / DSM 44217 / BCRC 13729 / KCTC 9384;
RX PubMed=12617516; DOI=10.7164/antibiotics.55.1063;
RA Onaka H., Taniguchi S., Igarashi Y., Furumai T.;
RT "Cloning of the staurosporine biosynthetic gene cluster from Streptomyces
RT sp. TP-A0274 and its heterologous expression in Streptomyces lividans.";
RL J. Antibiot. 55:1063-1071(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 39243 / DSM 44217 / BCRC 13729 / KCTC 9384;
RX PubMed=12512086; DOI=10.1002/cbic.200390004;
RA Hyun C.G., Bililign T., Liao J., Thorson J.S.;
RT "The biosynthesis of indolocarbazoles in a heterologous E. coli host.";
RL ChemBioChem 4:114-117(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 39243 / DSM 44217 / BCRC 13729 / KCTC 9384;
RX PubMed=15743957; DOI=10.1128/jb.187.6.2084-2092.2005;
RA Nishizawa T., Aldrich C.C., Sherman D.H.;
RT "Molecular analysis of the rebeccamycin L-amino acid oxidase from
RT Lechevalieria aerocolonigenes ATCC 39243.";
RL J. Bacteriol. 187:2084-2092(2005).
RN [6]
RP FUNCTION.
RC STRAIN=ATCC 39243 / DSM 44217 / BCRC 13729 / KCTC 9384;
RX PubMed=12619684; DOI=10.1271/bbb.67.127;
RA Onaka H., Taniguchi S., Igarashi Y., Furumai T.;
RT "Characterization of the biosynthetic gene cluster of rebeccamycin from
RT Lechevalieria aerocolonigenes ATCC 39243.";
RL Biosci. Biotechnol. Biochem. 67:127-138(2003).
RN [7]
RP CATALYTIC ACTIVITY.
RC STRAIN=ATCC 39243 / DSM 44217 / BCRC 13729 / KCTC 9384;
RX PubMed=16575939; DOI=10.1002/cbic.200500504;
RA Zhang C., Albermann C., Fu X., Peters N.R., Chisholm J.D., Zhang G.,
RA Gilbert E.J., Wang P.G., Van Vranken D.L., Thorson J.S.;
RT "RebG- and RebM-catalyzed indolocarbazole diversification.";
RL ChemBioChem 7:795-804(2006).
CC -!- FUNCTION: Catalyzes the penultimate step in the biosynthesis of
CC rebeccamycin, an indolocarbazole alkaloid that inhibits topoisomerase
CC 1. Has a wide substrate range, including staurosporine aglycone, EJG-
CC III-108A, J-104303, 6-N-methyl-arcyriaflavin and indolo-[2,3-a]-
CC carbazole. {ECO:0000269|PubMed:10866221, ECO:0000269|PubMed:12619684}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4'-demethylrebeccamycin + H2O = beta-D-glucose +
CC dichloroarcyriaflavin A; Xref=Rhea:RHEA:27397, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15903, ChEBI:CHEBI:330772, ChEBI:CHEBI:595389;
CC EC=4.3.3.5; Evidence={ECO:0000269|PubMed:16575939};
CC -!- PATHWAY: Alkaloid biosynthesis.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC {ECO:0000305}.
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DR EMBL; AB023953; BAA83130.1; -; Genomic_DNA.
DR EMBL; AJ414559; CAC93713.1; -; Genomic_DNA.
DR EMBL; AB071405; BAC15749.1; -; Genomic_DNA.
DR EMBL; AF534707; AAN01207.1; -; Genomic_DNA.
DR EMBL; AB090952; BAC10673.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8KHE4; -.
DR SMR; Q8KHE4; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR KEGG; ag:BAC10673; -.
DR BioCyc; MetaCyc:MON-15083; -.
DR BRENDA; 4.3.3.5; 4340.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:InterPro.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProt.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR006326; UDPGT_MGT-like.
DR Pfam; PF00201; UDPGT; 1.
DR TIGRFAMs; TIGR01426; MGT; 1.
PE 1: Evidence at protein level;
KW Lyase.
FT CHAIN 1..421
FT /note="4'-demethylrebeccamycin synthase"
FT /id="PRO_0000424220"
FT CONFLICT 270..275
FT /note="ANDAER -> GQRRGT (in Ref. 1; BAA83130)"
FT /evidence="ECO:0000305"
FT CONFLICT 348
FT /note="Missing (in Ref. 1; BAA83130)"
FT /evidence="ECO:0000305"
FT CONFLICT 350
FT /note="A -> R (in Ref. 1; BAA83130)"
FT /evidence="ECO:0000305"
FT CONFLICT 414..421
FT /note="VDLIEGLV -> ST (in Ref. 1; BAA83130)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 421 AA; 46021 MW; B4D74C14DAD0BBD9 CRC64;
MGARVLVATT PGDGHVNPMV PVAQEMVSRG HEVRWYTGKA FRSTVERTGA RHEPMRDAHD
FGGMPREEAF PQHAGLTGIT GMIAGFRDIF IEPAADQMTD LLALLEDFPA DVLVTDETFF
GAGFVSERTG IPVAWIATSI YVFSSRDTAP LGLGLPPSSS RLGRLRNTVL KQLTDRVVMR
DLRRHADVVR DRVGLPRIRK GAFENIMRTP DLYLLGTVPS FEYPRGDMPP EVRFVGPFVS
PAPPDFTPPA WWGELDSGRP VVHVTQGTVA NDAERLLLPA IRALAAEDVL VVATTGAPLE
LEPMPANVRV ERFIPHHALL PHVDAMVTNG GYGGVNTALA HGVPLVVAAA TEEKHEVAAR
VSWSGAGVHL KKRRLSERDI RRAVRAVLDE PRFRVHAARL RDEYAARDAV VDAVDLIEGL
V
