REC10_SCHPO
ID REC10_SCHPO Reviewed; 791 AA.
AC Q09823;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Linear element protein rec10 {ECO:0000305};
DE AltName: Full=Meiotic recombination protein rec10;
GN Name=rec10 {ECO:0000312|PomBase:SPAC25G10.04c};
GN ORFNames=SPAC25G10.04c {ECO:0000312|PomBase:SPAC25G10.04c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=7586030; DOI=10.1007/bf00311213;
RA Lin Y., Smith G.R.;
RT "Molecular cloning of the meiosis-induced rec10 gene of Schizosaccharomyces
RT pombe.";
RL Curr. Genet. 27:440-446(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP IDENTIFICATION IN THE LINEAR ELEMENT COMPLEX, SUBCELLULAR LOCATION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=23395004; DOI=10.1016/j.molcel.2013.01.008;
RA Fowler K.R., Gutierrez-Velasco S., Martin-Castellanos C., Smith G.R.;
RT "Protein determinants of meiotic DNA break hot spots.";
RL Mol. Cell 49:983-996(2013).
RN [4]
RP MUTAGENESIS OF 176-VAL--GLY-178; 184-ARG-ASP-185; GLU-271; ARG-434 AND
RP GLY-727.
RX PubMed=28469148; DOI=10.1038/s41598-017-00742-3;
RA Ma L., Fowler K.R., Martin-Castellanos C., Smith G.R.;
RT "Functional organization of protein determinants of meiotic DNA break
RT hotspots.";
RL Sci. Rep. 7:1393-1393(2017).
RN [5]
RP FUNCTION, INTERACTION WITH HOP1; REC15 AND REC25, SUBCELLULAR LOCATION,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLU-309 AND 348-PRO-LYS-349.
RX PubMed=31665745; DOI=10.1093/nar/gkz754;
RA Kariyazono R., Oda A., Yamada T., Ohta K.;
RT "Conserved HORMA domain-containing protein Hop1 stabilizes interaction
RT between proteins of meiotic DNA break hotspots and chromosome axis.";
RL Nucleic Acids Res. 47:10166-10180(2019).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=33825974; DOI=10.1007/s00412-021-00757-w;
RA Ding D.Q., Matsuda A., Okamasa K., Hiraoka Y.;
RT "Linear elements are stable structures along the chromosome axis in fission
RT yeast meiosis.";
RL Chromosoma 130:s00412.021.00757-s00412.021.00757(2021).
CC -!- FUNCTION: Organizes linear element components on chromosomes and is
CC thus required for meiotic DNA recombination.
CC {ECO:0000269|PubMed:31665745, ECO:0000269|PubMed:33825974,
CC ECO:0000269|PubMed:7586030}.
CC -!- SUBUNIT: Component of linear elements (LinEs), which are similar to
CC synaptonemal complexes, at least composed of rec27, rec25, rec10 and
CC mug20 (PubMed:23395004). Interacts with rec25; the interaction is
CC direct (PubMed:31665745). Interacts with hop1 (via N-terminus); the
CC interaction is direct (PubMed:31665745). Interacts with rec15 (via C-
CC terminus); the interaction is direct (PubMed:31665745).
CC {ECO:0000269|PubMed:23395004, ECO:0000269|PubMed:31665745}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:31665745, ECO:0000269|PubMed:33825974}. Chromosome
CC {ECO:0000269|PubMed:31665745}. Note=Localizes to DNA double-strand
CC break (DSB) hotspots. {ECO:0000269|PubMed:31665745}.
CC -!- DEVELOPMENTAL STAGE: Present from pre-meiotic DNA replication and
CC disappears following meiotic prophase I. {ECO:0000269|PubMed:33825974,
CC ECO:0000269|PubMed:7586030}.
CC -!- DISRUPTION PHENOTYPE: Abnormal linear element formation
CC (PubMed:33825974, PubMed:23395004). Abolishes localization of hop1 to
CC meiotic chromosomal axis sites and DNA double-strand break (DSB)
CC hotspots (PubMed:31665745). Severely decreases localization of rec15 to
CC chromosomal axis sites (PubMed:31665745). Abnormal sporulation
CC (PubMed:33825974). {ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:31665745, ECO:0000269|PubMed:33825974}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA99538.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U09871; AAA99538.1; ALT_INIT; Genomic_DNA.
DR EMBL; CU329670; CAA94633.1; -; Genomic_DNA.
DR PIR; S55725; S55725.
DR PIR; T52489; T52489.
DR RefSeq; NP_594524.1; NM_001019953.2.
DR AlphaFoldDB; Q09823; -.
DR BioGRID; 278184; 18.
DR STRING; 4896.SPAC25G10.04c.1; -.
DR PaxDb; Q09823; -.
DR PRIDE; Q09823; -.
DR EnsemblFungi; SPAC25G10.04c.1; SPAC25G10.04c.1:pep; SPAC25G10.04c.
DR GeneID; 2541688; -.
DR KEGG; spo:SPAC25G10.04c; -.
DR PomBase; SPAC25G10.04c; rec10.
DR VEuPathDB; FungiDB:SPAC25G10.04c; -.
DR HOGENOM; CLU_354176_0_0_1; -.
DR OMA; HRDFACR; -.
DR PRO; PR:Q09823; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0030998; C:linear element; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IMP:PomBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:PomBase.
DR InterPro; IPR012491; Red1/Rec10.
DR Pfam; PF07964; Red1; 1.
PE 1: Evidence at protein level;
KW Chromosome; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..791
FT /note="Linear element protein rec10"
FT /id="PRO_0000097220"
FT REGION 462..523
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 644..672
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 485..492
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 462..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 502..516
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 176..178
FT /note="VIG->IID: Decreases meiotic gene conversion at ade6
FT and leads to abnormal localization of rec25 within the
FT nucleus."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 184..185
FT /note="RD->FT: Decreases meiotic gene conversion at ade6,
FT crossing-over between ade6 and arg1, and leads to abnormal
FT localization of rec25 to the nucleus."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 271
FT /note="E->K: Decreases meiotic gene conversion at ade6;
FT when associated with H-434."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 309
FT /note="E->A: Decreases interaction with rec15. Decreases
FT meiotic gene conversion at ade6. Decreases spore
FT viability."
FT /evidence="ECO:0000269|PubMed:31665745"
FT MUTAGEN 348..349
FT /note="PK->GG: Decreases interaction with hop1. Decreases
FT meiotic gene conversion at ade6."
FT /evidence="ECO:0000269|PubMed:31665745"
FT MUTAGEN 434
FT /note="R->H: Decreases meiotic gene conversion at ade6;
FT when associated with K-271."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 727
FT /note="G->E: Abnormal localization of rec25 within the
FT nucleus."
FT /evidence="ECO:0000269|PubMed:28469148"
FT CONFLICT 202
FT /note="S -> F (in Ref. 1; AAA99538)"
FT /evidence="ECO:0000305"
FT CONFLICT 257
FT /note="S -> A (in Ref. 1; AAA99538)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 791 AA; 89876 MW; 682DFA66B5FFA7B9 CRC64;
MDIFSEFLNC LSSDGTLNES SIYKTYQILE SLNPKDVDTK ENYIKLSNTF STLGSGVGFQ
DNLLIEMFKI LTVLFFKTRS TDLGDLLIES FTSLEIEKLM RVKKTIGSIV LTKGIQELQE
IELPKVGFNC MTYDESIFQG ISLERLILQL MSIFIAKCEE NKLWLVNNRK DLDLRVIGQR
LLHRDFACRF LSGLFISRFS VSGDTDESKH QNGIRLQLFI DFSKFETRLT MQILKADRIF
STCVANTVHA YEGLFSSGSN IDSTIATLVL EPRDLIVYRK GFALLQIPWT SVTTIDKIKK
VKSLKIITEV SSLDEFVFQC KDGDKFDELF STSEEIMNKL LPAIIVSPKL TLRNRSIIQI
KEGESKLPNT SKQASQNLPH LDDELAYQRF EDQVIDKSVC DDECTNTENT PSSNIPADVK
DSLSADDYAY DTKRKTQIED LEEDQNKSKI ASKDGTNLKE INSVPEFSDE NVINQTGPAK
KTPVQRRKDG KFAKSTKRKK QKSLKPDTEN QESSVKNKKA KSNVNLQYSP KTPICKINDE
TLKPPTIANI AGHKQMNHLT SENIETPVPV PNGNWYNGVK HETATDIFTT CHDGNNSLKS
SVWKELLKEK HWKQESKPQL TGNSRQIDLS TFVKQANTPN ITSLLDGTCS SPPNNECFND
KEPDSSSSTL ISDRQELEYR NPNAETVKLE EIPYNKFFKT VEKNEAYNPS SKSATIDGLQ
RYTSMIGNQI YEGILQNEKE LRSKLEAYHI NCNKVIKEFS KRQTARYKII EKELAQIEVN
LVSQIDSLMF K
