REC1_USTMA
ID REC1_USTMA Reviewed; 463 AA.
AC P14746; A0A0D1E2G6; Q4P933; Q99110;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=DNA repair exonuclease REC1;
DE EC=3.1.11.2;
GN Name=REC1; ORFNames=UMAG_11016;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2602156; DOI=10.1093/nar/17.24.10489;
RA Holden D.W., Spanos A., Banks G.R.;
RT "Nucleotide sequence of the REC1 gene of Ustilago maydis.";
RL Nucleic Acids Res. 17:10489-10489(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=8276878; DOI=10.1016/s0021-9258(17)42413-6;
RA Thelen M.P., Onel K., Holloman W.K.;
RT "The REC1 gene of Ustilago maydis involved in the cellular response to DNA
RT damage encodes an exonuclease.";
RL J. Biol. Chem. 269:747-754(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=521 / FGSC 9021;
RA Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION OF INTRON.
RX PubMed=7565682; DOI=10.1128/mcb.15.10.5329;
RA Onel K., Thelen M.P., Ferguson D.O., Bennett R.L., Holloman W.K.;
RT "Mutation avoidance and DNA repair proficiency in Ustilago maydis are
RT differentially lost with progressive truncation of the REC1 gene product.";
RL Mol. Cell. Biol. 15:5329-5338(1995).
CC -!- FUNCTION: Plays a central role in regulating the genetic system of this
CC fungus. Has a 3'-->5' exonuclease activity.
CC {ECO:0000269|PubMed:8276878}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC nucleoside 5'-phosphates.; EC=3.1.11.2;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Cells are very sensitive to UV light.
CC {ECO:0000269|PubMed:8276878}.
CC -!- SIMILARITY: Belongs to the rad1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA35660.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X17663; CAA35660.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U01836; AAB60310.1; -; Genomic_DNA.
DR EMBL; CM003147; KIS68815.1; -; Genomic_DNA.
DR PIR; S06912; RGUS1M.
DR RefSeq; XP_011389835.1; XM_011391533.1.
DR AlphaFoldDB; P14746; -.
DR SMR; P14746; -.
DR STRING; 5270.UM03380P0; -.
DR EnsemblFungi; KIS68815; KIS68815; UMAG_11016.
DR GeneID; 23566952; -.
DR KEGG; uma:UMAG_11016; -.
DR VEuPathDB; FungiDB:UMAG_11016; -.
DR eggNOG; KOG3194; Eukaryota.
DR InParanoid; P14746; -.
DR OrthoDB; 1440961at2759; -.
DR Proteomes; UP000000561; Chromosome 8.
DR GO; GO:0030896; C:checkpoint clamp complex; IBA:GO_Central.
DR GO; GO:0008853; F:exodeoxyribonuclease III activity; IEA:UniProtKB-EC.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR InterPro; IPR003021; Rad1_Rec1_Rad17.
DR InterPro; IPR003027; Rec1_exonuc_Ustilaginaceae.
DR PANTHER; PTHR10870; PTHR10870; 1.
DR Pfam; PF02144; Rad1; 1.
DR PRINTS; PR01245; RAD1REC1.
DR PRINTS; PR01247; RECEXONCLASE.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; Exonuclease; Hydrolase; Nuclease; Nucleus;
KW Reference proteome.
FT CHAIN 1..463
FT /note="DNA repair exonuclease REC1"
FT /id="PRO_0000097223"
FT REGION 81..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 172..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 334
FT /note="N -> K (in Ref. 1; CAA35660 and 2; AAB60310)"
FT /evidence="ECO:0000305"
FT CONFLICT 354
FT /note="E -> Q (in Ref. 1; CAA35660)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 463 AA; 50127 MW; B509C9090159F61A CRC64;
MPAEGACDAA SLMTLTATLS DVTGLANLLK SVAIQTHAVV IASSSGLEII TELNRTLQAH
AYLYSHMFDS YRFENAQDDV RGSTSLQARS RPKKRSKLTS KHAETADSQS SAASSDHESG
QSQAHTKKRF RANSHSYAQE ADRVHDEPDS VSFEVNLQTW ISCLNIFGGV GPSRPHSSSS
GLPGFRPEQG SAEAPPGGRG YQRTRYGVAD AYGAERGTSV ERGFDRNPFS SSAKATRMKL
SYQGHGNPLV LELEQDANVL TRVSMSTYEP SFLTDMVFEP QNMVAQVIVA SELMQSAFTE
IDASCKKLSI LITSPHSLST YDGDQRTEAP APTNRNTSAS MLKFRAISDT GSSEMEFPAS
LTSSDPTGVI EKFVALPGSS EQWYDFTLLS RTMSVLRSSI KTSLRMDEAG LISFQFMMPK
YRRAAAAGAP LTNAAAGQAA HEDEQDAFCE FLCCPLDTST LIV
