REC25_SCHPO
ID REC25_SCHPO Reviewed; 150 AA.
AC Q9C115;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Linear element protein rec25 {ECO:0000305};
DE AltName: Full=Meiotic recombination protein rec25;
DE AltName: Full=Meiotically up-regulated gene 19 protein;
GN Name=rec25 {ECO:0000312|PomBase:SPAC17A5.18c};
GN Synonyms=mug19 {ECO:0000312|PomBase:SPAC17A5.18c};
GN ORFNames=SPAC17A5.18c {ECO:0000312|PomBase:SPAC17A5.18c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
RN [5]
RP FUNCTION, IDENTIFICATION IN THE LINEAR ELEMENT COMPLEX, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=23395004; DOI=10.1016/j.molcel.2013.01.008;
RA Fowler K.R., Gutierrez-Velasco S., Martin-Castellanos C., Smith G.R.;
RT "Protein determinants of meiotic DNA break hot spots.";
RL Mol. Cell 49:983-996(2013).
RN [6]
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF ASN-36; ASN-63; LEU-90; LYS-124
RP AND LEU-137.
RX PubMed=28469148; DOI=10.1038/s41598-017-00742-3;
RA Ma L., Fowler K.R., Martin-Castellanos C., Smith G.R.;
RT "Functional organization of protein determinants of meiotic DNA break
RT hotspots.";
RL Sci. Rep. 7:1393-1393(2017).
RN [7]
RP INTERACTION WITH REC10.
RX PubMed=31665745; DOI=10.1093/nar/gkz754;
RA Kariyazono R., Oda A., Yamada T., Ohta K.;
RT "Conserved HORMA domain-containing protein Hop1 stabilizes interaction
RT between proteins of meiotic DNA break hotspots and chromosome axis.";
RL Nucleic Acids Res. 47:10166-10180(2019).
RN [8]
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=33825974; DOI=10.1007/s00412-021-00757-w;
RA Ding D.Q., Matsuda A., Okamasa K., Hiraoka Y.;
RT "Linear elements are stable structures along the chromosome axis in fission
RT yeast meiosis.";
RL Chromosoma 130:s00412.021.00757-s00412.021.00757(2021).
CC -!- FUNCTION: During meiotic DNA recombination, binds to and may help
CC activate DNA double-strand break (DSB) hotspot sites.
CC {ECO:0000269|PubMed:16303567, ECO:0000269|PubMed:23395004}.
CC -!- SUBUNIT: Component of linear elements (LinEs), which are similar to
CC synaptonemal complexes, at least composed of rec27, rec25, rec10 and
CC mug20 (PubMed:23395004). Interacts with rec10; the interaction is
CC direct (PubMed:31665745). {ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:31665745}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372, ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:28469148, ECO:0000269|PubMed:33825974}. Chromosome
CC {ECO:0000269|PubMed:23395004}. Note=Localizes to chromosomal double-
CC strand break (DSB) hotspot sites during the meiotic cell cycle.
CC {ECO:0000269|PubMed:23395004}.
CC -!- DEVELOPMENTAL STAGE: Present from pre-meiotic DNA replication and
CC disappears following meiotic prophase I. {ECO:0000269|PubMed:33825974}.
CC -!- DISRUPTION PHENOTYPE: Abnormal linear element formation
CC (PubMed:33825974, PubMed:23395004). Abnormal sporulation
CC (PubMed:33825974). {ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:33825974}.
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DR EMBL; CU329670; CAC34941.1; -; Genomic_DNA.
DR RefSeq; NP_593469.1; NM_001018902.2.
DR AlphaFoldDB; Q9C115; -.
DR SMR; Q9C115; -.
DR BioGRID; 278853; 5.
DR STRING; 4896.SPAC17A5.18c.1; -.
DR iPTMnet; Q9C115; -.
DR MaxQB; Q9C115; -.
DR PaxDb; Q9C115; -.
DR EnsemblFungi; SPAC17A5.18c.1; SPAC17A5.18c.1:pep; SPAC17A5.18c.
DR GeneID; 2542389; -.
DR KEGG; spo:SPAC17A5.18c; -.
DR PomBase; SPAC17A5.18c; rec25.
DR VEuPathDB; FungiDB:SPAC17A5.18c; -.
DR HOGENOM; CLU_1741641_0_0_1; -.
DR OMA; LLMCMES; -.
DR PRO; PR:Q9C115; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0030998; C:linear element; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:1903343; P:positive regulation of meiotic DNA double-strand break formation; EXP:PomBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:PomBase.
DR GO; GO:0062209; P:spatial regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination; EXP:PomBase.
PE 1: Evidence at protein level;
KW Chromosome; Chromosome partition; Cytoplasm; Meiosis; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..150
FT /note="Linear element protein rec25"
FT /id="PRO_0000297689"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MUTAGEN 36
FT /note="N->D: Decreases meiotic gene conversion at ade6, and
FT crossing-over between ade6 and arg1; when associated with
FT P-137."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 63
FT /note="N->I: Decreases meiotic gene conversion at ade6,
FT crossing-over between ade6 and arg1, and abolishes double-
FT strand break (DSB) formation at a DSB hotspot."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 90
FT /note="L->P: Decreases meiotic gene conversion at ade6, and
FT crossing-over between ade6 and arg1."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 124
FT /note="K->I: Decreases meiotic gene conversion at ade6, and
FT crossing-over between ade6 and arg1."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 137
FT /note="L->P: Decreases meiotic gene conversion at ade6, and
FT crossing-over between ade6 and arg1; when associated with
FT D-36."
FT /evidence="ECO:0000269|PubMed:28469148"
SQ SEQUENCE 150 AA; 17096 MW; 19FF292069BC947F CRC64;
MVKGSVTNTS SIVKQFEKSS IKHETETIAF AKQFINECIR EFQEQKDNVV EEKNTDQNHQ
NQNQEGVIIE IYQELLQKVD LISSELRQNL SSLQLRFQEV ERDTGHDLLN VLNSLSQEAR
LAQKVLEEDG SQQGSQLIQG LLTCFQSTGN
