REC27_SCHPO
ID REC27_SCHPO Reviewed; 125 AA.
AC Q9USR4;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 2.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Linear element protein rec27 {ECO:0000305};
DE AltName: Full=Meiotic recombination protein rec27;
DE AltName: Full=Meiotically up-regulated gene 41 protein;
GN Name=rec27 {ECO:0000312|PomBase:SPBC577.05c}; Synonyms=mug41;
GN ORFNames=SPBC577.05c {ECO:0000312|PomBase:SPBC577.05c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP FUNCTION, IDENTIFICATION IN THE LINEAR ELEMENT COMPLEX, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=23395004; DOI=10.1016/j.molcel.2013.01.008;
RA Fowler K.R., Gutierrez-Velasco S., Martin-Castellanos C., Smith G.R.;
RT "Protein determinants of meiotic DNA break hot spots.";
RL Mol. Cell 49:983-996(2013).
RN [5]
RP IDENTIFICATION OF INITIATOR METHIONINE, FUNCTION, AND MUTAGENESIS OF
RP LYS-28; ARG-32; LYS-36 AND GLN-119.
RX PubMed=28469148; DOI=10.1038/s41598-017-00742-3;
RA Ma L., Fowler K.R., Martin-Castellanos C., Smith G.R.;
RT "Functional organization of protein determinants of meiotic DNA break
RT hotspots.";
RL Sci. Rep. 7:1393-1393(2017).
RN [6]
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=33825974; DOI=10.1007/s00412-021-00757-w;
RA Ding D.Q., Matsuda A., Okamasa K., Hiraoka Y.;
RT "Linear elements are stable structures along the chromosome axis in fission
RT yeast meiosis.";
RL Chromosoma 130:s00412.021.00757-s00412.021.00757(2021).
CC -!- FUNCTION: During meiotic DNA recombination, binds to and activates DNA
CC double-strand break (DSB) hotspot sites. {ECO:0000269|PubMed:16303567,
CC ECO:0000269|PubMed:23395004, ECO:0000269|PubMed:28469148}.
CC -!- SUBUNIT: Component of linear elements (LinEs), which are similar to
CC synaptonemal complexes, at least composed of rec27, rec25, rec10 and
CC mug20. {ECO:0000269|PubMed:23395004}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372, ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:33825974}. Chromosome {ECO:0000269|PubMed:23395004}.
CC Note=Localizes to chromosomal double-strand break (DSB) hotspot sites
CC during the meiotic cell cycle. {ECO:0000269|PubMed:23395004}.
CC -!- DEVELOPMENTAL STAGE: Present from pre-meiotic DNA replication and
CC disappears following meiotic prophase I. {ECO:0000269|PubMed:33825974}.
CC -!- DISRUPTION PHENOTYPE: Abnormal linear element formation
CC (PubMed:33825974, PubMed:23395004). Strongly decreases DNA double-
CC strand break formation during the meiotic cell cycle (PubMed:23395004).
CC Abnormal sporulation (PubMed:33825974). {ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:33825974}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB54813.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:28469148};
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DR EMBL; CU329671; CAB54813.1; ALT_INIT; Genomic_DNA.
DR PIR; T40549; T40549.
DR RefSeq; NP_595303.1; NM_001021210.2.
DR AlphaFoldDB; Q9USR4; -.
DR BioGRID; 277598; 3.
DR STRING; 4896.SPBC577.05c.1; -.
DR PaxDb; Q9USR4; -.
DR EnsemblFungi; SPBC577.05c.1; SPBC577.05c.1:pep; SPBC577.05c.
DR GeneID; 2541083; -.
DR KEGG; spo:SPBC577.05c; -.
DR PomBase; SPBC577.05c; rec27.
DR VEuPathDB; FungiDB:SPBC577.05c; -.
DR HOGENOM; CLU_1897430_0_0_1; -.
DR OMA; HMERCIR; -.
DR PRO; PR:Q9USR4; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0030998; C:linear element; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0031490; F:chromatin DNA binding; IDA:PomBase.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:1903343; P:positive regulation of meiotic DNA double-strand break formation; EXP:PomBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:PomBase.
DR GO; GO:0062209; P:spatial regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination; EXP:PomBase.
PE 1: Evidence at protein level;
KW Chromosome; Chromosome partition; Coiled coil; Cytoplasm; Meiosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..125
FT /note="Linear element protein rec27"
FT /id="PRO_0000297692"
FT COILED 45..104
FT /evidence="ECO:0000255"
FT MUTAGEN 28
FT /note="K->E: Decreases meiotic gene conversion at ade6 and
FT double-strand break (DSB) formation at a DSB hotspot."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 32
FT /note="R->E: Decreases meiotic gene conversion at ade6, and
FT crossing-over between ade6 and arg1."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 36
FT /note="K->E: Decreases meiotic gene conversion at ade6,
FT crossing-over between ade6 and arg1, and double-strand
FT break (DSB) formation at a DSB hotspot."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 119
FT /note="Q->K: Strongly decreases meiotic gene conversion at
FT ade6, crossing-over between ade6 and arg1, and abolishes
FT double-strand break (DSB) formation at a DSB hotspot."
FT /evidence="ECO:0000269|PubMed:28469148"
SQ SEQUENCE 125 AA; 14843 MW; A3AD404EB056DBD7 CRC64;
MKKRSETLGS DQSSSEIIEH VLEELNLKNI ERRVKKYDQI ESEYKTNIEN EKKAFIKDVS
QVQQKIKEFE IQKANQIKQL NEEKLSIEAR KQQLEIEIRN QLLQYAEKLR IVVKTPMNQP
TNTEV
