REC3_ARATH
ID REC3_ARATH Reviewed; 1608 AA.
AC F4HZK4; Q9XI40;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Protein REDUCED CHLOROPLAST COVERAGE 3 {ECO:0000303|PubMed:26862170};
GN Name=REC3 {ECO:0000303|PubMed:26862170};
GN OrderedLocusNames=At1g15290 {ECO:0000312|Araport:AT1G15290};
GN ORFNames=F9L1.23 {ECO:0000312|EMBL:AAD39657.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26862170; DOI=10.1073/pnas.1515741113;
RA Larkin R.M., Stefano G., Ruckle M.E., Stavoe A.K., Sinkler C.A.,
RA Brandizzi F., Malmstrom C.M., Osteryoung K.W.;
RT "REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana help to
RT establish the size of the chloroplast compartment.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:E1116-E1125(2016).
CC -!- FUNCTION: May act as the scaffold of a protein complex, which
CC sequesters key factors that are required for the G2 to M transition in
CC meristematic tissues (By similarity). Together with REC2, REC3 and
CC FMT/CLU, contributes to the establishment of the cellular volume
CC devoted to the chloroplast compartment (PubMed:26862170).
CC {ECO:0000250|UniProtKB:F4JKH6, ECO:0000269|PubMed:26862170}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:F4HS99,
CC ECO:0000255|PROSITE-ProRule:PRU00768}. Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:F4HS99}.
CC -!- DISRUPTION PHENOTYPE: Reduced proportion of the cellular volume devoted
CC to chloroplasts leading to an abnormal chloroplasts distributions
CC (PubMed:26862170). Lower levels of chlorophyll, especially in plants
CC lacking REC1, REC2, REC3 and FMT/CLU (PubMed:26862170).
CC {ECO:0000269|PubMed:26862170}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD39657.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007591; AAD39657.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29300.1; -; Genomic_DNA.
DR PIR; B86287; B86287.
DR RefSeq; NP_172981.3; NM_101398.5.
DR AlphaFoldDB; F4HZK4; -.
DR SMR; F4HZK4; -.
DR STRING; 3702.AT1G15290.1; -.
DR iPTMnet; F4HZK4; -.
DR PaxDb; F4HZK4; -.
DR PRIDE; F4HZK4; -.
DR ProMEX; F4HZK4; -.
DR ProteomicsDB; 199369; -.
DR EnsemblPlants; AT1G15290.1; AT1G15290.1; AT1G15290.
DR GeneID; 838097; -.
DR Gramene; AT1G15290.1; AT1G15290.1; AT1G15290.
DR Araport; AT1G15290; -.
DR TAIR; locus:2037783; AT1G15290.
DR eggNOG; KOG1839; Eukaryota.
DR HOGENOM; CLU_001902_1_1_1; -.
DR InParanoid; F4HZK4; -.
DR OrthoDB; 60958at2759; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HZK4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0019750; P:chloroplast localization; IMP:UniProtKB.
DR GO; GO:0006996; P:organelle organization; IEA:InterPro.
DR CDD; cd15466; CLU-central; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR033646; CLU-central.
DR InterPro; IPR025697; CLU_dom.
DR InterPro; IPR027523; CLU_prot.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR12601; PTHR12601; 1.
DR Pfam; PF12807; eIF3_p135; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS51823; CLU; 1.
DR PROSITE; PS50005; TPR; 3.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..1608
FT /note="Protein REDUCED CHLOROPLAST COVERAGE 3"
FT /id="PRO_0000453546"
FT DOMAIN 288..564
FT /note="Clu"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01167"
FT REPEAT 848..881
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 890..923
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 932..965
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT REPEAT 974..1007
FT /note="TPR 4"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 278..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1194..1226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1238..1292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1369..1400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1466..1499
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1531..1552
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1217..1224
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 1239..1268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1275..1292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1480..1499
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1608 AA; 177634 MW; 5E99910945BD769C CRC64;
MAPRSSKGKS NNKGKGGDKK KRDDKLLAPS LVEITVTTPY ETQVILKGVS TDKIIDVRRL
LASHVETCHF TNYSLSHKVK GHKLNDNIQV LSLKPCFLRM IPEEYLEESQ ALTQVRRVID
IVACTTRFFS KSPNKSIVAG NANPTPAPDG LDMVAIHTTP KLSQFYEFFS IHHLSPPILH
LKKVDGEEAG EKRDGDYFGL KVKICNGKVI HVIASVKGFF AVGKQLSHCH SIVDLLQNVS
NAFAKAYESL MKAFTDRNKF GNLPFGLRSN TWLVPSPVSE SASPLPTEDE HWGGNGGGQG
RNGEYDHRPW AAEFSVLATL PCKTEEERVI RDKKAFLLHS QFIDTSVQRA VRAICNVMDT
NQQTSGTTDL PAGSILLEDH VGDLSIVVKR DIASLDSKPE ATFQNDAFVL SSEELAERNL
LKGITADESV IVHDTPALGK VIVRQCGYTA VVNVKGQTQK AMSDFRDILI DDLPDGGANA
LNLNSLRVEF HRPHSVGTSV ENQPTQLDWD DLESYRCIIQ ELVKINLTKL EETRVSSVRP
IRWELGSTWV QHLQKKETDV CGKPATNDET ELSVKGLGKQ FKDLKSKSKK SENISAVNEK
DTRLHELNEE DDLGQKSIDG LFTELKELLS EEAFSRLKET GTGLHLKSKE ELTNMAYGYY
DEIALPRLVA DFGSLELSPV DGRTLTDFMH IRGLQMRSLG HVAKLAEKLP HIQSLCIHEM
ITRAFKHLLR AVIASVNNMA ELPVAVAASL NFMLGRRELE GCDRIPGEEY CLRLQWLQKF
LSRKFGWIQK DEFHHLKKFS ILRGLCQKVG LELVSRDFDF DSPNPFMSSD IIGLVPVCKH
VLCISSDGRT LLESSKLALD KGKLDDAVSY GTKALVKMIA VCGPYHRNTA CAYSLLAVVL
YHTGDFNQAT IYQQKALDIN ERELGLDHPD TMKSYGDLSV FYYRLQHFEL ALKYVNRALF
LLHFTCGLSH PNTAATYINV AMMEKEVGND HLALRYLHEA LKSNKRLLGA DHIQTAASYH
AIAVALSFME AHSLSVQHEQ TTLQILTAKL GADDLRTQDA AAWLEYFESR AIEQQEAGRN
GIPKPDASIA SKGHLSVSDL LDYISSDPDT KGNVAHRKHR RARILQVNDK VASADDDAHR
VASQIDIVTW NNVAEADVTK SRSEVNDPDT VVDKTNIETG DIVVHRLNVD RQTVEESTLD
EGWQEAYSKG RSGNGAGRKS RQRQPDLMKK RMLLNKHHNR NQDVQQQNIY SPLQKTSKGP
SLSKSSPRRA LKNAEIDVST NTTKPQLKAS GAAAVTSTTL ASKSLSYKEV ALAPPGTVLK
PMLEKLELNL ERTETQIYRT SSASSGEESK SDTVMLDLPI EGTELHCEKQ ESQESAESVE
NLTSESEGDL GSYRGKKTSD ISRTKLSASA EPYNPGGFLV IDLQSSAATI GSYPIMVADP
ISWAVVSCGI HSPPYYSAIH SNGVGTPRSM NPDAPEFVPR RSLQNSSQHA GEDASVSVDS
SSCLKAEKDA VDLKKRELAS FIVKSSQKEV PAALSKTSPE AESGGTSEKD SAVTEIVYSR
EEENGANANE TNGGEGFVIV AKKRRRKNKV RLTNVAAGLY HQPSSVCA
