REC8_RAT
ID REC8_RAT Reviewed; 593 AA.
AC Q6AYJ4; Q6QQR2;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Meiotic recombination protein REC8 homolog;
DE AltName: Full=Cohesin Rec8p;
GN Name=Rec8 {ECO:0000250|UniProtKB:Q8C5S7};
GN Synonyms=Rec8L1 {ECO:0000312|EMBL:AAH79023.1};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000312|EMBL:AAS20426.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar {ECO:0000312|EMBL:AAS20426.1};
RA Ben-Ari V., Dekel N.;
RT "Rat Rec8 mRNA full sequence.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|EMBL:AAH79023.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Brown Norway {ECO:0000312|EMBL:AAH79023.1};
RC TISSUE=Testis {ECO:0000312|EMBL:AAH79023.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3] {ECO:0000305}
RP INTERACTION WITH RAD51, SUBCELLULAR LOCATION, AND PHOSPHORYLATION.
RX PubMed=12615909; DOI=10.1083/jcb.200212080;
RA Eijpe M., Offenberg H., Jessberger R., Revenkova E., Heyting C.;
RT "Meiotic cohesin REC8 marks the axial elements of rat synaptonemal
RT complexes before cohesins SMC1beta and SMC3.";
RL J. Cell Biol. 160:657-670(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-164 AND SER-192, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Required during meiosis for separation of sister chromatids
CC and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome
CC arms by separin during anaphase I allows for homologous chromosome
CC separation in meiosis I and cleavage of REC8 on centromeres during
CC anaphase II allows for sister chromatid separation in meiosis II (By
CC similarity). {ECO:0000250|UniProtKB:Q8C5S7}.
CC -!- SUBUNIT: Interacts (phosphorylated and unphosphorylated form) with
CC SMC3. Interacts with SYCP3. Interacts (phosphorylated and
CC unphosphorylated form) with SMC1B. Does not interact with SMC1A (By
CC similarity). Interacts with RAD51. Forms a complex with EWSR1, PRDM9,
CC SYCP3 and SYCP1; complex formation is dependent of phosphorylated form
CC of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with
CC the chromosomal axis through REC8 (By similarity). {ECO:0000250,
CC ECO:0000250|UniProtKB:Q8C5S7, ECO:0000269|PubMed:12615909}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12615909}. Chromosome
CC {ECO:0000269|PubMed:12615909}. Chromosome, centromere
CC {ECO:0000269|PubMed:12615909}. Note=In meiotic chromosomes, localized
CC along axial elements in prophase from the leptotene to diplotene
CC stages. At later prophase stages, diakinesis and metaphase I, localized
CC along interstitial axes of chromosomes including both centromere and
CC arm regions. No longer detected in arm regions in anaphase I but
CC persists on centromere regions until metaphase II.
CC {ECO:0000269|PubMed:12615909}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:12615909}.
CC -!- SIMILARITY: Belongs to the rad21 family. {ECO:0000255}.
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DR EMBL; AY529701; AAS20426.1; -; mRNA.
DR EMBL; BC079023; AAH79023.1; -; mRNA.
DR RefSeq; NP_001011916.1; NM_001011916.1.
DR AlphaFoldDB; Q6AYJ4; -.
DR SMR; Q6AYJ4; -.
DR STRING; 10116.ENSRNOP00000026430; -.
DR iPTMnet; Q6AYJ4; -.
DR PhosphoSitePlus; Q6AYJ4; -.
DR PaxDb; Q6AYJ4; -.
DR PRIDE; Q6AYJ4; -.
DR Ensembl; ENSRNOT00000026430; ENSRNOP00000026430; ENSRNOG00000019503.
DR GeneID; 290227; -.
DR KEGG; rno:290227; -.
DR UCSC; RGD:1309376; rat.
DR CTD; 9985; -.
DR RGD; 1309376; Rec8.
DR eggNOG; KOG1213; Eukaryota.
DR GeneTree; ENSGT00390000011379; -.
DR HOGENOM; CLU_036680_0_0_1; -.
DR InParanoid; Q6AYJ4; -.
DR OMA; RVYFQQC; -.
DR OrthoDB; 1582475at2759; -.
DR PhylomeDB; Q6AYJ4; -.
DR TreeFam; TF338144; -.
DR PRO; PR:Q6AYJ4; -.
DR Proteomes; UP000002494; Chromosome 15.
DR Bgee; ENSRNOG00000019503; Expressed in testis and 9 other tissues.
DR GO; GO:0008278; C:cohesin complex; IBA:GO_Central.
DR GO; GO:0000793; C:condensed chromosome; ISO:RGD.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; ISO:RGD.
DR GO; GO:0000794; C:condensed nuclear chromosome; ISO:RGD.
DR GO; GO:0000776; C:kinetochore; ISO:RGD.
DR GO; GO:0000800; C:lateral element; ISO:RGD.
DR GO; GO:0001673; C:male germ cell nucleus; ISO:RGD.
DR GO; GO:0030893; C:meiotic cohesin complex; ISO:RGD.
DR GO; GO:0034991; C:nuclear meiotic cohesin complex; ISO:RGD.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0000795; C:synaptonemal complex; ISO:RGD.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISO:RGD.
DR GO; GO:0009566; P:fertilization; ISO:RGD.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; ISO:RGD.
DR GO; GO:0007141; P:male meiosis I; ISO:RGD.
DR GO; GO:0051321; P:meiotic cell cycle; ISO:RGD.
DR GO; GO:0051177; P:meiotic sister chromatid cohesion; IBA:GO_Central.
DR GO; GO:0001556; P:oocyte maturation; ISO:RGD.
DR GO; GO:0072520; P:seminiferous tubule development; ISO:RGD.
DR GO; GO:0007062; P:sister chromatid cohesion; IBA:GO_Central.
DR GO; GO:0007286; P:spermatid development; ISO:RGD.
DR GO; GO:0007130; P:synaptonemal complex assembly; ISO:RGD.
DR InterPro; IPR039781; Rad21/Rec8-like.
DR InterPro; IPR006909; Rad21/Rec8_C_eu.
DR InterPro; IPR006910; Rad21_Rec8_N.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12585; PTHR12585; 1.
DR Pfam; PF04824; Rad21_Rec8; 1.
DR Pfam; PF04825; Rad21_Rec8_N; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW Centromere; Chromosome; Chromosome partition; Meiosis; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..593
FT /note="Meiotic recombination protein REC8 homolog"
FT /id="PRO_0000308168"
FT REGION 247..282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 317..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 422..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 247..265
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..331
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 149
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8C5S7"
FT MOD_RES 164
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 192
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CONFLICT 435..441
FT /note="Missing (in Ref. 1; AAS20426)"
FT /evidence="ECO:0000305"
FT CONFLICT 490
FT /note="W -> WW (in Ref. 1; AAS20426)"
FT /evidence="ECO:0000305"
FT CONFLICT 523
FT /note="Missing (in Ref. 1; AAS20426)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 593 AA; 67874 MW; F16504E35995E92D CRC64;
MFYYPNVLQR HTGCFATIWL AATRGSRLVK REYLKVNVVK TCEEILNYVL VRVQPPMPGL
PRPRFSLYLS AQLQIGVIRV YFQQCQYLVE DIQHILEHLH RAQLRIRIDM EEADLPSLLL
PNCLAMMETL EDAPEPFFGT MSVDPTLPSP FDIPQIRHLL EAATPEKIRE ETLPEATPEP
RKPDRTLAMV QSPEVITLQE AEPIRMLRIE GEQDLPEISR GDLDLLIAEE DDAILLEERQ
RGRLLRQRRA SPPLDESKEE PRALEGDGLV SSLSPPAPAQ VEGIREPLPD QAFPPEVQKM
PGWEPGVLLT EVTPPQELRL PAPPSIEKRP PSPQRPPRRR RRGRQLLFWD KETQISREKF
EEQLQTGAHC CEYPVVQPPK RKLMSPAELF RTPTLSGWLS PELLALWTHC AQVPPRMLRQ
RPQLETEETV EEERVADKEE RRKTEALSEI EVLREAQEPS GPLMLSSELS LEVAEEEKSR
TSFIPPEERW AWIEERQPEP PALPMLPELP EVPMEMPPGP ELLSSEAVLR AVALELQANR
EPDFSSLVPP LSSRKLASRV FYLLLVLSAQ KILLVDQQKP YGRLLIRLGP KFP
