REC8_SCHPO
ID REC8_SCHPO Reviewed; 561 AA.
AC P36626; O60051;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Meiotic recombination protein rec8;
DE AltName: Full=Cohesin rec8;
GN Name=rec8; ORFNames=SPBC29A10.14;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND
RP PHOSPHORYLATION.
RX PubMed=10207075; DOI=10.1128/mcb.19.5.3515;
RA Parisi S., McKay M.J., Molnar M., Thompson M.A., van der Spek P.J.,
RA van Drunen-Schoenmaker E., Kanaar R., Lehmann E., Hoeijmakers J.H.J.,
RA Kohli J.;
RT "Rec8p, a meiotic recombination and sister chromatid cohesion
RT phosphoprotein of the Rad21p family conserved from fission yeast to
RT humans.";
RL Mol. Cell. Biol. 19:3515-3528(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND PHOSPHORYLATION.
RX PubMed=10440376; DOI=10.1038/22774;
RA Watanabe Y., Nurse P.;
RT "Cohesin Rec8 is required for reductional chromosome segregation at
RT meiosis.";
RL Nature 400:461-464(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 147-561.
RX PubMed=1339382; DOI=10.1093/genetics/132.1.75;
RA Lin Y., Larson K.L., Dorer R., Smith G.R.;
RT "Meiotically induced rec7 and rec8 genes of Schizosaccharomyces pombe.";
RL Genetics 132:75-85(1992).
RN [5]
RP INTERACTION WITH MOA1.
RX PubMed=16325576; DOI=10.1016/j.cell.2005.09.013;
RA Yokobayashi S., Watanabe Y.;
RT "The kinetochore protein moa1 enables cohesion-mediated monopolar
RT attachment at meiosis I.";
RL Cell 123:803-817(2005).
RN [6]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=31665745; DOI=10.1093/nar/gkz754;
RA Kariyazono R., Oda A., Yamada T., Ohta K.;
RT "Conserved HORMA domain-containing protein Hop1 stabilizes interaction
RT between proteins of meiotic DNA break hotspots and chromosome axis.";
RL Nucleic Acids Res. 47:10166-10180(2019).
CC -!- FUNCTION: Involved primarily in the early steps of meiotic
CC recombination. Required to ensure reductional chromosome segregation.
CC {ECO:0000269|PubMed:10440376, ECO:0000269|PubMed:16303567}.
CC -!- SUBUNIT: Interacts with moa1. {ECO:0000269|PubMed:16325576}.
CC -!- INTERACTION:
CC P36626; Q9UTI4: moa1; NbExp=2; IntAct=EBI-1564281, EBI-1564258;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10207075,
CC ECO:0000269|PubMed:16823372, ECO:0000269|PubMed:31665745}. Chromosome
CC {ECO:0000269|PubMed:31665745}. Note=Localizes to meiotic chromosomal
CC axis sites. {ECO:0000269|PubMed:31665745}.
CC -!- DEVELOPMENTAL STAGE: Abundant at 3 hours after induction of meiosis but
CC undetectable before induction or at 4 hours and later.
CC -!- PTM: Phosphorylated during prophase until after meiosis I.
CC {ECO:0000269|PubMed:10207075, ECO:0000269|PubMed:10440376}.
CC -!- SIMILARITY: Belongs to the rad21 family. {ECO:0000305}.
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DR EMBL; AJ223299; CAA11240.1; -; Genomic_DNA.
DR EMBL; AB018077; BAA33496.1; -; mRNA.
DR EMBL; CU329671; CAA22442.1; -; Genomic_DNA.
DR EMBL; M85298; AAB04034.1; -; Genomic_DNA.
DR PIR; T43428; T43428.
DR RefSeq; NP_596059.1; NM_001021970.2.
DR AlphaFoldDB; P36626; -.
DR SMR; P36626; -.
DR BioGRID; 276986; 23.
DR IntAct; P36626; 1.
DR STRING; 4896.SPBC29A10.14.1; -.
DR PaxDb; P36626; -.
DR EnsemblFungi; SPBC29A10.14.1; SPBC29A10.14.1:pep; SPBC29A10.14.
DR GeneID; 2540458; -.
DR KEGG; spo:SPBC29A10.14; -.
DR PomBase; SPBC29A10.14; rec8.
DR VEuPathDB; FungiDB:SPBC29A10.14; -.
DR eggNOG; KOG1213; Eukaryota.
DR HOGENOM; CLU_438030_0_0_1; -.
DR InParanoid; P36626; -.
DR OMA; CANFYEY; -.
DR PhylomeDB; P36626; -.
DR PRO; PR:P36626; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0008278; C:cohesin complex; IBA:GO_Central.
DR GO; GO:0000939; C:inner kinetochore; IDA:PomBase.
DR GO; GO:0072687; C:meiotic spindle; IDA:PomBase.
DR GO; GO:0034991; C:nuclear meiotic cohesin complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0005721; C:pericentric heterochromatin; IDA:PomBase.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:PomBase.
DR GO; GO:1990813; P:meiotic centromeric cohesion protection; IMP:PomBase.
DR GO; GO:0010789; P:meiotic sister chromatid cohesion involved in meiosis I; IMP:PomBase.
DR GO; GO:0051754; P:meiotic sister chromatid cohesion, centromeric; IBA:GO_Central.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IMP:PomBase.
DR GO; GO:0007062; P:sister chromatid cohesion; IBA:GO_Central.
DR Gene3D; 1.10.10.580; -; 1.
DR InterPro; IPR039781; Rad21/Rec8-like.
DR InterPro; IPR006909; Rad21/Rec8_C_eu.
DR InterPro; IPR006910; Rad21_Rec8_N.
DR InterPro; IPR023093; ScpA-like_C.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12585; PTHR12585; 1.
DR Pfam; PF04824; Rad21_Rec8; 1.
DR Pfam; PF04825; Rad21_Rec8_N; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW Chromosome; Meiosis; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..561
FT /note="Meiotic recombination protein rec8"
FT /id="PRO_0000097880"
FT REGION 223..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 401..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..242
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 401..421
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 531..561
FT /note="SLATKSAFLVKQDKPYSEISVSLNLKSTDAI -> CKYHNQTTN (in
FT Ref. 4; AAB04034)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 561 AA; 64011 MW; E6B9B3A02143D50A CRC64;
MFYNQDVLTK EKGGMGVIWL AATLGSKHSL RKLHKKDIMS VDIDEACDFV AFSPEPLALR
LSSNLMIGVT RVWAHQYSFF HSQVSTLHLR VRKELDHFTS KPFKNIDIQN EQTNPKQLLL
AEDPAFIPEV SLYDAFNLPS VDLHVDMSSF TQPKENPNIS VLETLPDSTS YLINTSQNYS
LRNNVSSFVY EDSRAFSTEE PLDFEFDENG DIQELTKGTI NSDPSLQAAS QHSNLGSVQR
EYNSEEQESR IHMFEIDEDV LPLPVPLQSV MDSEHNENEP RALKRRKVQK LLEPDENIEL
STRTLSQWRK NYVERMIALE ATKYVRRRGA SSAKKKELNK FFDWESFHPL LKPWIEKLKP
SNNTPSEIDD VLRNIDTSEV EVGRDVQGEL GLNIPWNTSS RSNSAINSKS HSQTGSEHST
PLLDTKYRKR LPHSPSMPSR VEFLPALESS QFHETLNSEL SLQLSDDFVL YKNTQEENAH
LMLSMEKECA NFYEYAKTAI YENNGRITFS SLLPNDLKRP VVAQAFSHLL SLATKSAFLV
KQDKPYSEIS VSLNLKSTDA I
