RECAC_ARATH
ID RECAC_ARATH Reviewed; 439 AA.
AC Q39199; Q39200;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=DNA repair protein recA homolog 1, chloroplastic;
DE AltName: Full=Recombinase A homolog 1;
DE Flags: Precursor;
GN Name=RECA; OrderedLocusNames=At1g79050; ORFNames=YUP8H12R.33, YUP8H12R_18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=8029344; DOI=10.1104/pp.103.2.673;
RA Binet M.N., Osman M., Jagendorf A.T.;
RT "Genomic nucleotide sequence of a gene from Arabidopsis thaliana encoding a
RT protein homolog of Escherichia coli RecA.";
RL Plant Physiol. 103:673-674(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 2-439.
RC STRAIN=cv. Columbia;
RX PubMed=1518831; DOI=10.1073/pnas.89.17.8068;
RA Cerutti H.D., Osman M., Grandoni P., Jagendorf A.T.;
RT "A homolog of Escherichia coli RecA protein in plastids of higher plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:8068-8072(1992).
CC -!- FUNCTION: Involved in recombination ability and DNA strand transfer
CC activity.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q39199-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the RecA family. {ECO:0000305}.
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DR EMBL; L15229; AAA61781.1; -; Genomic_DNA.
DR EMBL; AC002986; AAC17051.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36197.1; -; Genomic_DNA.
DR EMBL; AY050885; AAK92822.1; -; mRNA.
DR EMBL; AY113960; AAM45008.1; -; mRNA.
DR EMBL; M98039; AAA32855.1; -; mRNA.
DR PIR; A46259; A46259.
DR PIR; T01050; T01050.
DR RefSeq; NP_565198.1; NM_106556.2. [Q39199-1]
DR AlphaFoldDB; Q39199; -.
DR SMR; Q39199; -.
DR STRING; 3702.AT1G79050.1; -.
DR PaxDb; Q39199; -.
DR PRIDE; Q39199; -.
DR ProteomicsDB; 236869; -. [Q39199-1]
DR EnsemblPlants; AT1G79050.1; AT1G79050.1; AT1G79050. [Q39199-1]
DR GeneID; 844246; -.
DR Gramene; AT1G79050.1; AT1G79050.1; AT1G79050. [Q39199-1]
DR KEGG; ath:AT1G79050; -.
DR Araport; AT1G79050; -.
DR TAIR; locus:2207445; AT1G79050.
DR eggNOG; KOG1433; Eukaryota.
DR InParanoid; Q39199; -.
DR OMA; EGARHMS; -.
DR PhylomeDB; Q39199; -.
DR PRO; PR:Q39199; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q39199; baseline and differential.
DR Genevisible; Q39199; AT.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:TAIR.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR CDD; cd00983; recA; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00268; RecA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013765; DNA_recomb/repair_RecA.
DR InterPro; IPR020584; DNA_recomb/repair_RecA_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR023400; RecA_C.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR PANTHER; PTHR45900; PTHR45900; 1.
DR Pfam; PF00154; RecA; 1.
DR PRINTS; PR00142; RECA.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54752; SSF54752; 1.
DR TIGRFAMs; TIGR02012; tigrfam_recA; 1.
DR PROSITE; PS00321; RECA_1; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Chloroplast; DNA damage;
KW DNA recombination; DNA-binding; Nucleotide-binding; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 53..439
FT /note="DNA repair protein recA homolog 1, chloroplastic"
FT /id="PRO_0000030275"
FT REGION 413..439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 142..149
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 439 AA; 47733 MW; 35465E98F7B37E15 CRC64;
MDSQLVLSLK LNPSFTPLSP LFPFTPCSSF SPSLRFSSCY SRRLYSPVTV YAAKKLSHKI
SSEFDDRING ALSPDADSRF LDRQKALEAA MNDINSSFGK GSVTRLGSAG GALVETFSSG
ILTLDLALGG GLPKGRVVEI YGPESSGKTT LALHAIAEVQ KLGGNAMLVD AEHAFDPAYS
KALGVDVENL IVCQPDNGEM ALETADRMCR SGAVDLICVD SVSALTPRAE IEGEIGMQQM
GLQARLMSQA LRKMSGNASK AGCTLIFLNQ IRYKIGVYYG NPEVTSGGIA LKFFASVRLE
IRSAGKIKSS KGDEDIGLRA RVRVQKSKVS RPYKQAEFEI MFGEGVSKLG CVLDCAEIME
VVVKKGSWYS YEDQRLGQGR EKALQHLREN PALQDEIEKK VRLLMLDGEV HRSTPLMSSS
SSSASHREEE EEDSLDDFQ
