RECAM_ARATH
ID RECAM_ARATH Reviewed; 389 AA.
AC Q8RY99; Q9SR78;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=DNA repair protein recA homolog 2, mitochondrial;
DE AltName: Full=Recombinase A homolog 2;
DE Flags: Precursor;
GN OrderedLocusNames=At3g10140; ORFNames=T22K18.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX PubMed=12768414; DOI=10.1007/s00438-003-0859-6;
RA Khazi F.R., Edmondson A.C., Nielsen B.L.;
RT "An Arabidopsis homologue of bacterial RecA that complements an E. coli
RT recA deletion is targeted to plant mitochondria.";
RL Mol. Genet. Genomics 269:454-463(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Involved in recombination ability and DNA strand transfer
CC activity. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:12768414}.
CC -!- SIMILARITY: Belongs to the RecA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF04410.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY072877; AAL79768.1; -; mRNA.
DR EMBL; AC010927; AAF04410.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74864.1; -; Genomic_DNA.
DR EMBL; AY136400; AAM97066.1; -; mRNA.
DR EMBL; BT000214; AAN15533.1; -; mRNA.
DR RefSeq; NP_187625.2; NM_111849.4.
DR AlphaFoldDB; Q8RY99; -.
DR SMR; Q8RY99; -.
DR BioGRID; 5511; 3.
DR IntAct; Q8RY99; 3.
DR STRING; 3702.AT3G10140.1; -.
DR PaxDb; Q8RY99; -.
DR PRIDE; Q8RY99; -.
DR ProteomicsDB; 236224; -.
DR EnsemblPlants; AT3G10140.1; AT3G10140.1; AT3G10140.
DR GeneID; 820177; -.
DR Gramene; AT3G10140.1; AT3G10140.1; AT3G10140.
DR KEGG; ath:AT3G10140; -.
DR Araport; AT3G10140; -.
DR TAIR; locus:2100038; AT3G10140.
DR eggNOG; KOG1433; Eukaryota.
DR HOGENOM; CLU_040469_3_2_1; -.
DR InParanoid; Q8RY99; -.
DR OMA; EASDCEL; -.
DR OrthoDB; 1158328at2759; -.
DR PhylomeDB; Q8RY99; -.
DR PRO; PR:Q8RY99; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RY99; baseline and differential.
DR Genevisible; Q8RY99; AT.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IGI:TAIR.
DR GO; GO:0009408; P:response to heat; IGI:TAIR.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR013765; DNA_recomb/repair_RecA.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR023400; RecA_C.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR PANTHER; PTHR45900; PTHR45900; 1.
DR Pfam; PF00154; RecA; 1.
DR PRINTS; PR00142; RECA.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54752; SSF54752; 1.
DR TIGRFAMs; TIGR02012; tigrfam_recA; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; DNA damage; DNA recombination; DNA-binding; Mitochondrion;
KW Nucleotide-binding; Reference proteome; Transit peptide.
FT TRANSIT 1..15
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 16..389
FT /note="DNA repair protein recA homolog 2, mitochondrial"
FT /id="PRO_0000045859"
FT BINDING 123..130
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 389 AA; 42954 MW; 24D9762A881F27CC CRC64;
MGRLSWASPI QRFRFFSYLS QLNGRRSVLA CSGYENRYLS SLVEASDCEL DEVPDDRKVA
EKDTALHLAL SQLSGDFDKD SKLSLQRFYR KRRVSVISTG SLNLDLALGV GGLPKGRMVE
VYGKEASGKT TLALHIIKEA QKLGGYCAYL DAENAMDPSL AESIGVNTEE LLISRPSSAE
KMLNIVDVLT KSGSVDVIVV DSVAALAPQC ELDAPVGERY RDTQSRIMTQ ALRKIHYSVG
YSQTLIVFLN QVRSHVKSNM HFPHAEEVTC GGNALPFHAA IRLKMIRTGL IKTANKISGL
NVCVQVVKNK LAPGKKKSEL GIHFGHGFYV EREVLELACE HGVILREGTS YFIEGEVIEG
KDAAEKYLVE NKEALDTVVA ILRNQLFKM
