RECA_HELPY
ID RECA_HELPY Reviewed; 347 AA.
AC P42445;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein RecA {ECO:0000255|HAMAP-Rule:MF_00268};
DE AltName: Full=Recombinase A {ECO:0000255|HAMAP-Rule:MF_00268};
GN Name=recA {ECO:0000255|HAMAP-Rule:MF_00268}; OrderedLocusNames=HP_0153;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 53726 / 84-183;
RX PubMed=7768597; DOI=10.1128/iai.63.6.2185-2193.1995;
RA Thompson S.A., Blaser M.J.;
RT "Isolation of the Helicobacter pylori recA gene and involvement of the recA
RT region in resistance to low pH.";
RL Infect. Immun. 63:2185-2193(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, AND SUGGESTION OF A
RP POST-TRANSLATIONAL MODIFICATION.
RC STRAIN=69A, and ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487;
RX PubMed=7476856; DOI=10.1007/bf02423452;
RA Schmitt W., Odenbreit S., Heuermann D., Haas R.;
RT "Cloning of the Helicobacter pylori recA gene and functional
RT characterization of its product.";
RL Mol. Gen. Genet. 248:563-572(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
CC -!- FUNCTION: Can catalyze the hydrolysis of ATP in the presence of single-
CC stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex
CC DNA, and the ATP-dependent hybridization of homologous single-stranded
CC DNAs. It interacts with LexA causing its activation and leading to its
CC autocatalytic cleavage.
CC -!- FUNCTION: Deletion of this gene leads to the inability of the bacteria
CC to perform homologous recombination, and markedly increases UV
CC sensitivity.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00268}.
CC -!- PTM: The protein migrates as a 40 kDa protein in strains 69A and NCTC
CC 11637. When overexpressed in E.coli a 38 kDa protein is made which is
CC unable to complement the E.coli deletion mutant. It has been suggested
CC this size difference is due to a post-translational modification.
CC -!- SIMILARITY: Belongs to the RecA family. {ECO:0000255|HAMAP-
CC Rule:MF_00268}.
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DR EMBL; Z35478; CAA84615.1; -; Genomic_DNA.
DR EMBL; U13756; AAC43379.1; -; Genomic_DNA.
DR EMBL; AE000511; AAD07218.1; -; Genomic_DNA.
DR PIR; S58683; S58683.
DR RefSeq; NP_206952.1; NC_000915.1.
DR RefSeq; WP_000963128.1; NC_018939.1.
DR AlphaFoldDB; P42445; -.
DR SMR; P42445; -.
DR STRING; 85962.C694_00765; -.
DR PaxDb; P42445; -.
DR PRIDE; P42445; -.
DR EnsemblBacteria; AAD07218; AAD07218; HP_0153.
DR KEGG; hpy:HP_0153; -.
DR PATRIC; fig|85962.47.peg.166; -.
DR eggNOG; COG0468; Bacteria.
DR OMA; DYGEQAL; -.
DR PhylomeDB; P42445; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IMP:CACAO.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR CDD; cd00983; recA; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00268; RecA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013765; DNA_recomb/repair_RecA.
DR InterPro; IPR020584; DNA_recomb/repair_RecA_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR023400; RecA_C.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR PANTHER; PTHR45900; PTHR45900; 1.
DR Pfam; PF00154; RecA; 1.
DR PRINTS; PR00142; RECA.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54752; SSF54752; 1.
DR TIGRFAMs; TIGR02012; tigrfam_recA; 1.
DR PROSITE; PS00321; RECA_1; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; DNA damage; DNA recombination; DNA repair;
KW DNA-binding; Nucleotide-binding; Reference proteome; SOS response.
FT CHAIN 1..347
FT /note="Protein RecA"
FT /id="PRO_0000122725"
FT BINDING 67..74
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00268"
FT VARIANT 40
FT /note="S -> A (in strain: 69A and ATCC 53726)"
FT VARIANT 103
FT /note="H -> Y (in strain: ATCC 53726)"
FT VARIANT 113
FT /note="E -> Q (in strain: 69A)"
FT VARIANT 121
FT /note="D -> S (in strain: ATCC 53726)"
FT VARIANT 125
FT /note="Q -> E (in strain: ATCC 53726)"
FT VARIANT 203
FT /note="M -> T (in strain: ATCC 53726)"
FT VARIANT 232
FT /note="S -> T (in strain: 69A)"
FT VARIANT 319
FT /note="D -> N (in strain: ATCC 53726)"
SQ SEQUENCE 347 AA; 37686 MW; C8417BA54ACD27D8 CRC64;
MAIDEDKQKA ISLAIKQIDK VFGKGALVRL GDKQVEKIDS ISTGSLGLDL ALGIGGVPKG
RIIEIYGPES SGKTTLSLHI IAECQKNGGV CAFIDAEHAL DVHYAKRLGV DTENLLVSQP
DTGEQALEIL ETITRSGGID LVVVDSVAAL TPKAEIDGDM GDQHVGLQAR LMSHALRKIT
GVLHKMNTTL IFINQIRMKI GMMGYGSPET TTGGNALKFY ASVRIDIRRI ASLKQNEQHI
GNRAKAKVVK NKVAPPFREA EFDIMFGEGI SKEGEIIDYG VKLDIVDKSG AWLSYQDKKL
GQGRENAKAL LKEDKALADE ITLKIKESIG SNEEIMPLPD EPLEEME
