RECA_MYCFV
ID RECA_MYCFV Reviewed; 423 AA.
AC Q9F415;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 23-FEB-2022, entry version 103.
DE RecName: Full=Protein RecA;
DE AltName: Full=Recombinase A;
DE Contains:
DE RecName: Full=Mfl RecA intein;
DE Flags: Fragment;
GN Name=recA;
OS Mycolicibacterium flavescens (Mycobacterium flavescens).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=1776;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 14474 / DSM 43991 / NCTC 10271 / NRRL B-4038 / TMC 1541;
RX PubMed=11034333; DOI=10.1016/s0014-5793(00)01944-x;
RA Saves I., Laneelle M.-A., Daffe M., Masson J.-M.;
RT "Inteins invading mycobacterial RecA proteins.";
RL FEBS Lett. 480:221-225(2000).
CC -!- FUNCTION: Can catalyze the hydrolysis of ATP in the presence of single-
CC stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex
CC DNA, and the ATP-dependent hybridization of homologous single-stranded
CC DNAs. It interacts with LexA causing its activation and leading to its
CC autocatalytic cleavage (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RecA family. {ECO:0000305}.
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DR EMBL; AJ251150; CAC09590.1; -; Genomic_DNA.
DR STRING; 1776.BHQ18_23685; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008094; F:ATP-dependent activity, acting on DNA; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR Gene3D; 3.10.28.10; -; 2.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR013765; DNA_recomb/repair_RecA.
DR InterPro; IPR020584; DNA_recomb/repair_RecA_CS.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR PANTHER; PTHR45900; PTHR45900; 1.
DR Pfam; PF03161; LAGLIDADG_2; 1.
DR Pfam; PF00154; RecA; 1.
DR PRINTS; PR00379; INTEIN.
DR PRINTS; PR00142; RECA.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS00321; RECA_1; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 3: Inferred from homology;
KW ATP-binding; Autocatalytic cleavage; Cytoplasm; DNA damage;
KW DNA recombination; DNA repair; DNA-binding; Nucleotide-binding;
KW Protein splicing; SOS response.
FT CHAIN <1..9
FT /note="Protein RecA, 1st part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030254"
FT CHAIN 10..373
FT /note="Mfl RecA intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030255"
FT CHAIN 374..>423
FT /note="Protein RecA, 2nd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030256"
FT NON_TER 1
FT NON_TER 423
SQ SEQUENCE 423 AA; 47333 MW; 8CF63171128DC0F4 CRC64;
REKIGVMFGC FNYSTRVQLA DRTTEKIGKI VTQKMDVEVL SYDPDTDRVV PRKVVNWFNN
GPAEQFLQFT VEKSGGNGKS QFAATPNHLI RTPGGWTEAG DLIAGDRVMA TEPHRLSDQQ
FQVVLGSLMG DGNLSPNRRD RNGVRFRMGH GAKQVDYLRW KTELLGNIKH STRVNDKGAT
FVDFTPLPEL AELQRAVYLG DGKKFLSEEY LKALTPLALA IWYMDDGAFT VRSKGLQERT
AGGSGRIEIC VEAMSEGTRV RLRDYLHDTH GLEVRLRQSG KAGKAVLVFS TASSAKFQEL
VAPYMAPSME YKLLPRFRGQ STVSPQFVES TQRLVPARIL DVHVKPHTRS MNRFDIEVEG
NHNYFVDGVM VHNSPETTTG GKALKFYASV RMDVRRIETL KDGXDAVGNR TRVKVVKNKV
SPP
