ID   RECA_MYCGS              Reviewed;         419 AA.
AC   Q9F414;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Protein RecA;
DE   AltName: Full=Recombinase A;
DE   Contains:
DE     RecName: Full=Mga RecA intein;
DE   Flags: Fragment;
GN   Name=recA;
OS   Mycobacterium gastri.
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=1777;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HB4389;
RX   PubMed=11034333; DOI=10.1016/s0014-5793(00)01944-x;
RA   Saves I., Laneelle M.-A., Daffe M., Masson J.-M.;
RT   "Inteins invading mycobacterial RecA proteins.";
RL   FEBS Lett. 480:221-225(2000).
CC   -!- FUNCTION: Can catalyze the hydrolysis of ATP in the presence of single-
CC       stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex
CC       DNA, and the ATP-dependent hybridization of homologous single-stranded
CC       DNAs. It interacts with LexA causing its activation and leading to its
CC       autocatalytic cleavage (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RecA family. {ECO:0000305}.
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DR   EMBL; AJ251715; CAC09591.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9F414; -.
DR   PRIDE; Q9F414; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008094; F:ATP-dependent activity, acting on DNA; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 2.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR013765; DNA_recomb/repair_RecA.
DR   InterPro; IPR020584; DNA_recomb/repair_RecA_CS.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR020587; RecA_monomer-monomer_interface.
DR   PANTHER; PTHR45900; PTHR45900; 1.
DR   Pfam; PF03161; LAGLIDADG_2; 1.
DR   Pfam; PF00154; RecA; 1.
DR   PRINTS; PR00379; INTEIN.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
DR   TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR   TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
DR   PROSITE; PS00321; RECA_1; 1.
DR   PROSITE; PS50163; RECA_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Autocatalytic cleavage; Cytoplasm; DNA damage;
KW   DNA recombination; DNA repair; DNA-binding; Nucleotide-binding;
KW   Protein splicing; SOS response.
FT   CHAIN           <1..9
FT                   /note="Protein RecA, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030257"
FT   CHAIN           10..377
FT                   /note="Mga RecA intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030258"
FT   CHAIN           378..>419
FT                   /note="Protein RecA, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030259"
FT   NON_TER         1
FT   NON_TER         419
SQ   SEQUENCE   419 AA;  46602 MW;  4BB42A3BC12941E6 CRC64;
     RDKIGVMFGC GSWYTNVTLA DGSTEKLGKI VNQKMDVEVL SYDFESGQIV PRRVTNWFNN
     GKAEEFLHFK VDRAGSGTGR GHASLAMTRN HLIRTPVGWR EAEDINVGDR VMLAQPRLLS
     DQQWEIVLGS LMGDGCLSPP VRQDSESARL RIGHGAQQSA YFDWKVSLLA NIPHSRTVNG
     KGAAFVDFSP LAELHELRSA VYLGDGKKFL SEEYLKGLTP LSLAIWYMDD GSFSLRSKGL
     QQRTQGGSGR IEICVEAMSE GSQVRLRDYL HDTHGLDVRL RKAGAAAKAV LVFSTAATAK
     FQQLVAPYMA PCMAYKLLPR FHGRSMVTPQ FVEPIMELMP ARVTEIESKT DYPIMSRFDI
     EVEGSHNYFA DGVMVHNSPE TTTGGKALKF YASVRMDVQR IETLKDGTNT VDNRTRVKI