ID   RECA_MYCT3              Reviewed;         424 AA.
AC   Q9F407;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Protein RecA;
DE   AltName: Full=Recombinase A;
DE   Contains:
DE     RecName: Full=Mth RecA intein;
DE   Flags: Fragment;
GN   Name=recA;
OS   Mycolicibacterium thermoresistibile (strain ATCC 19527 / DSM 44167 / CIP
OS   105390 / JCM 6362 / NCTC 10409 / 316) (Mycobacterium thermoresistibile).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1078020;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 19527 / DSM 44167 / CIP 105390 / JCM 6362 / NCTC 10409 / 316;
RX   PubMed=11034333; DOI=10.1016/s0014-5793(00)01944-x;
RA   Saves I., Laneelle M.-A., Daffe M., Masson J.-M.;
RT   "Inteins invading mycobacterial RecA proteins.";
RL   FEBS Lett. 480:221-225(2000).
CC   -!- FUNCTION: Can catalyze the hydrolysis of ATP in the presence of single-
CC       stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex
CC       DNA, and the ATP-dependent hybridization of homologous single-stranded
CC       DNAs. It interacts with LexA causing its activation and leading to its
CC       autocatalytic cleavage (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RecA family. {ECO:0000305}.
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DR   EMBL; AJ251714; CAC09593.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9F407; -.
DR   STRING; 1078020.KEK_16623; -.
DR   eggNOG; COG0468; Bacteria.
DR   eggNOG; COG1372; Bacteria.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008094; F:ATP-dependent activity, acting on DNA; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 2.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR013765; DNA_recomb/repair_RecA.
DR   InterPro; IPR020584; DNA_recomb/repair_RecA_CS.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR020587; RecA_monomer-monomer_interface.
DR   PANTHER; PTHR45900; PTHR45900; 1.
DR   Pfam; PF03161; LAGLIDADG_2; 1.
DR   Pfam; PF00154; RecA; 1.
DR   PRINTS; PR00142; RECA.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
DR   TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR   TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
DR   PROSITE; PS00321; RECA_1; 1.
DR   PROSITE; PS50163; RECA_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Autocatalytic cleavage; Cytoplasm; DNA damage;
KW   DNA recombination; DNA repair; DNA-binding; Nucleotide-binding;
KW   Protein splicing; SOS response.
FT   CHAIN           <1..9
FT                   /note="Protein RecA, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030266"
FT   CHAIN           10..374
FT                   /note="Mth RecA intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030267"
FT   CHAIN           375..>424
FT                   /note="Protein RecA, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000030268"
FT   NON_TER         1
FT   NON_TER         424
SQ   SEQUENCE   424 AA;  46961 MW;  ACFFABBCA6CBBA88 CRC64;
     REKIGVMFGC FDYSTRAQLA DGTTEKIGKI VDNKMDVEVL SYDPDTDRIV PRKVVNWFNN
     GPAEQLLQFT VEKSGGNGRA RFAATPNHLI RTPGGWTEAG DLIAGDRVLA AEPHRLSDQQ
     FQIVLGSLMG DGTLSPDPRG RNGVRFRMGH GADRVDYLEW KTALLGNIKH STGENAEGAR
     FVDFTPLPEL AELRRAVYLG DDGRKFISEE YLKALTPLAL AIWYMDDGSL TVRSEGLQQG
     TAGGSGRIEI CVEAMTEGSR IRLRDHLRDT HGLDVRLRQA GAGGKAVLVF STAATAEFQE
     LVAPYMAPSM EYKLLPRFRG QSRVVPQFVE PTQRLVPARI LDVHVEPHTR SMNRYDIEVE
     GNHNYFVDGV MVHNSPETTT GGKALKFYAS VRIDVRRIET LKDGTEAVGN RTRAKIVKNK
     VSPP